Abstract
A relatively simple and rapid procedure for the measurement of free ammonium and the amides in plant extracts is described. The method was developed by combining a cation-exchange method for blood ammonia with a differential acid-hydrolysis procedure for asparagine and glutamine amide-nitrogen.
The recovery of standard samples (100-400 μg of ammonium- or amide-nitrogen) of free ammonium, asparagine, and glutamine after being run through the extraction, column, and analytical procedures ranged between 99 and 102%.
The harvest, extraction, and analytical procedures were tested on shoots from 4 to 6-day-old germinating barley seeds. The high levels of the amides and the low level of free ammonium present in the tissue extracts indicated that the extraction and analytical procedures resulted in little if any hydrolysis of the amides.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- RAMADAN M. E., GREENBERG D. M. AN ENZYMIC MICROMETHOD FOR DETERMINATION OF GLUTAMINE AND ASPARAGINE IN BLOOD. Anal Biochem. 1963 Aug;6:144–152. doi: 10.1016/0003-2697(63)90105-2. [DOI] [PubMed] [Google Scholar]
- SCHMIDT R. R. Nitrogen and phosphorus metabolism during synchronous growth of Chlorella pyrenoidosa. Exp Cell Res. 1961 Mar;23:209–217. doi: 10.1016/0014-4827(61)90031-3. [DOI] [PubMed] [Google Scholar]
- TOWER D. B., PETERS E. L., CURTIS W. C. Guinea pig serum L-asparaginase. Properties, purification, and application to determination of asparagine in biological samples. J Biol Chem. 1963 Mar;238:983–993. [PubMed] [Google Scholar]
- Vickery H. B., Pucher G. W., Clark H. E. The determination of glutamine in the presence of asparagine. Biochem J. 1935 Dec;29(12):2710–2720. doi: 10.1042/bj0292710. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZYDOWO M., PURZYCKA J. Enzymic determination of glutamine with rat kidney preparation. Acta Biochim Pol. 1963;10:293–299. [PubMed] [Google Scholar]