Abstract
The kinetics of development of protein-synthesizing capacity in the imbibing wheat embryo, were studied both in vivo and in vitro. During the first 30 minutes of imbibition protein-synthesizing capacity rises rapidly, lagging about 10 minutes behind water uptake. This rise in synthesizing capacity is accompanied by an increase in polysome content. As imbibition continues, both protein-synthesizing capacity and polysome content increase. With embryos from aged seed, the rate of protein synthesis is initially limited by another, presumably nonribosomal, reaction.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HENSHAW E. C., BOJARSKI T. B., HIATT H. H. PROTEIN SYNTHESIS BY FREE AND BOUND RAT LIVER RIBOSOMES IN VIVO AND IN VITRO. J Mol Biol. 1963 Aug;7:122–129. doi: 10.1016/s0022-2836(63)80041-8. [DOI] [PubMed] [Google Scholar]
- Hendler R. W. Lipoamino acids as precursors in the synthesis of authentic proteins by homogenates of hen oviduct. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1233–1240. doi: 10.1073/pnas.54.4.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARCUS A., FEELEY J. PROTEIN SYNTHESIS IN IMBIBED SEEDS. II. POLYSOME FORMATION DURING IMBIBITION. J Biol Chem. 1965 Apr;240:1675–1680. [PubMed] [Google Scholar]
- Marcus A., Feeley J. ACTIVATION OF PROTEIN SYNTHESIS IN THE IMBIBITION PHASE OF SEED GERMINATION. Proc Natl Acad Sci U S A. 1964 Jun;51(6):1075–1079. doi: 10.1073/pnas.51.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marrè E., Cocucci S., Sturani E. On the Development of the Ribosomal System in the Endosperm of Germinating Castor Bean Seeds. Plant Physiol. 1965 Nov;40(6):1162–1170. doi: 10.1104/pp.40.6.1162. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAFFORD D. W., SOFER W. H., IVERSON R. M. DEMONSTRATION OF POLYRIBOSOMES AFTER FERTILIZATION OF THE SEA URCHIN EGG. Proc Natl Acad Sci U S A. 1964 Aug;52:313–316. doi: 10.1073/pnas.52.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warner J. R., Rich A., Hall C. E. Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin. Science. 1962 Dec 28;138(3548):1399–1403. doi: 10.1126/science.138.3548.1399. [DOI] [PubMed] [Google Scholar]