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. 1990 Nov;87(22):8820–8824. doi: 10.1073/pnas.87.22.8820

Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin.

D Zhang 1, P Wadsworth 1, P K Hepler 1
PMCID: PMC55051  PMID: 11607116

Abstract

Carboxyfluorescein-labeled brain tubulin has been microinjected into stamen hair cells of Tradescantia, and its distribution during mitosis and cytokinesis was examined using confocal laser scanning fluorescence microscopy. The results show that brain tubulin incorporates into plant microtubules and is utilized throughout mitosis and cytokinesis. Microtubule structures that incorporate brain tubulin include the preprophase band, the perinuclear sheath at late prophase, the kinetochore fibers during prometaphase, metaphase, and anaphase, the interzone spindle during anaphase, and finally the phragmoplast during late anaphase and telophase. All of these microtubule-containing structures and, notably, their transitions from one to another have been observed in single live cells progressing through mitosis and cytokinesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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