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. 1966 Oct;41(8):1247–1253. doi: 10.1104/pp.41.8.1247

Protein and Nucleic Acid Metabolism in Fruits: I. Studies of Amino Acid Incorporation During the Climacteric Rise in Respiration of the Avocado 1

Amos Richmond 1,2, Jacob B Biale 1
PMCID: PMC550511  PMID: 5978544

Abstract

Incorporation of 14C L-valine and 14C L-leucine into protein of tissue slices of the avocado fruit was relatively high during the early stages of the climacteric rise, declined sharply thereafter, and was virtually absent at the peak. The incorporation of amino acids in the preclimacteric stage was markedly lower than during the early stage of the respiratory rise. By following incorporation in relation to uptake at several concentrations it was established that the results were not a reflection of endogenous dilution.

Puromycin was effective as an inhibitor of incorporation but not of oxygen uptake. When respiration was at its maximum there was no protein synthesis. It was concluded, therefore, that the respiratory upsurge characteristic of the climacteric was not related directly to protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biale J. B. Growth, Maturation, and Senescence in Fruits: Recent knowledge on growth regulation and on biological oxidations has been applied to studies with fruits. Science. 1964 Nov 13;146(3646):880–888. doi: 10.1126/science.146.3646.880. [DOI] [PubMed] [Google Scholar]
  2. Bieleski R. L., Laties G. G. Turnover Rates of Phosphate Esters in Fresh and Aged Slices of Potato Tuber Tissue. Plant Physiol. 1963 Sep;38(5):586–594. doi: 10.1104/pp.38.5.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLICK R. E., HACKETT D. P. THE ROLE OF PROTEIN AND NUCLEIC ACID SYNTHESIS IN THE DEVELOPMENT OF RESPIRATION IN POTATO TUBER SLICES. Proc Natl Acad Sci U S A. 1963 Aug;50:243–250. doi: 10.1073/pnas.50.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hobson G. E. Polygalacturonase in normal and abnormal tomato fruit. Biochem J. 1964 Aug;92(2):324–332. doi: 10.1042/bj0920324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Laties G. G. Controlling Influence of Thickness on Development & Type of Respiratory Activity in Potato Slices. Plant Physiol. 1962 Sep;37(5):679–690. doi: 10.1104/pp.37.5.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PEARSON J. A., ROBERTSON R. N. The physiology of growth in apple fruits. VI. The control of respiration rate and synthesis. Aust J Biol Sci. 1954 Feb;7(1):1–17. [PubMed] [Google Scholar]
  7. Richmond A., Biale J. B. Protein synthesis in avocado fruit tissue. Arch Biochem Biophys. 1966 Jul;115(1):211–214. doi: 10.1016/s0003-9861(66)81059-7. [DOI] [PubMed] [Google Scholar]
  8. Sacher J. A. Permeability Characteristics and Amino Acid Incorporation during Senescence (Ripening) of Banana Tissue. Plant Physiol. 1966 Apr;41(4):701–708. doi: 10.1104/pp.41.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Young R. E., Biale J. B. Carbon Dioxide Effects on Fruit Respiration. I. Measurement of Oxygen Uptake in Continuous Gas Flow. Plant Physiol. 1962 May;37(3):409–415. doi: 10.1104/pp.37.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Young R. E. Extraction of enzymes from tannin-bearing tissue. Arch Biochem Biophys. 1965 Jul;111(1):174–180. doi: 10.1016/0003-9861(65)90336-x. [DOI] [PubMed] [Google Scholar]

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