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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Virology. 2017 Mar 21;506:34–44. doi: 10.1016/j.virol.2017.03.002

Fig. 3.

Fig. 3

The HIV-1 ASP RNA inhibits viral replication. Jurkat cells were stably transduced with a pLVX-based lentivirus driving CMV promoter-dependent expression of the ASP RNA sequence with stops at codons 3, 7 and 10 of the ORF (J-ASTmut). As a control, we generated a Jurkat cell line stably transduced with the empty lentivirus (J-LVX). Panel A: Quantification of ASP RNA expression in parental Jurkat cells, J-LVX cells, and J-ASTmut cells. Equal amounts of total RNA from the three cell lines was utilized to measure expression of ASP RNA with the strand-specific RT-qPCR assay shown in Fig. 1. ND: not detected. Panel B: Parental Jurkat cells and the two stably transduced cell lines (J-LVX and J-ASTmut) were infected with equal amounts of HIV-1IIIB by spinoculation (m.o.i. 0.01), and cultured in complete medium. Viral replication was assessed by HIV-1 p24 ELISA over 10 days in culture. Graphs report average and standard deviation calculated from three replicate samples.