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. 1966 Oct;41(8):1364–1369. doi: 10.1104/pp.41.8.1364

Respiratory System of Rhodotorula glutinis I. Inhibitor Tolerance and Cytochrome Components

Shooichi Matsunaka 1,1, Sigehiro Morita 1,2, S F Conti 1,3,4,5
PMCID: PMC550532  PMID: 6009625

Abstract

Oxygen uptake by the carotenoid-containing yeast, Rhodotorula glutinis was not affected by concentrations of cyanide and antimycin A which completely inhibit the respiration of Saccharomyces cerevisiae. The tolerance of R. glutinis to these inhibitors was somewhat dependent on the age of the cultures. Reduced minus aerated difference spectra of cells revealed spectral changes presumably due to cytochromes and carotenoids. The kinetics of these spectral changes induced by oxygen were followed. Carotenoid deficient cells were prepared by growth in the presence of diphenylamine. Difference spectra of these cells revealed the presence of flavoprotein, and a, b, and c type cytochromes. Growth of R. glutinis was completely inhibited by concentrations of cyanide which did not affect respiration. Oxidation of reduced nicotinamide adenine dinucleotide by sub-cellular fractions was sensitive to cyanide and antimycin A. Although respiration of intact cells is tolerant to these inhibitors, studies with cell-free extracts suggest the presence of a cyanide and antimycin A-sensitive, cytochrome-linked, respiratory chain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANCE B. Spectra and reaction kinetics of respiratory pigments of homogenized and intact cells. Nature. 1952 Feb 9;169(4293):215–221. doi: 10.1038/169215a0. [DOI] [PubMed] [Google Scholar]
  2. CLARK L. C., Jr, WOLF R., GRANGER D., TAYLOR Z. Continuous recording of blood oxygen tensions by polarography. J Appl Physiol. 1953 Sep;6(3):189–193. doi: 10.1152/jappl.1953.6.3.189. [DOI] [PubMed] [Google Scholar]
  3. COHEN-BAZIRE G., STANIER R. Y. Specific inhibition of carotenoid synthesis in a photosynthetic bacterium and its physiological consequences. Nature. 1958 Jan 24;181(4604):250–252. doi: 10.1038/181250a0. [DOI] [PubMed] [Google Scholar]
  4. FULLER R. C., ANDERSON I. C. Suppression of carotenoid synthesis and its effect on the activity of photosynthetic bacterial chromatophores. Nature. 1958 Jan 24;181(4604):252–254. doi: 10.1038/181252a0. [DOI] [PubMed] [Google Scholar]
  5. LINDENMAYER A., ESTABROOK R. W. Low-temperature spectral studies on the biosynthesis of cytochromes in Baker's yeast. Arch Biochem Biophys. 1958 Nov;78(1):66–82. doi: 10.1016/0003-9861(58)90315-1. [DOI] [PubMed] [Google Scholar]
  6. PERSON P., ZIPPER H. SHIFT OF CYTOCHROME OXIDASE ALPHA-PEAK FROM 603-605 M-UPSILON TO 590 M-UPSILON BY HEAT DENATURATION. Biochim Biophys Acta. 1964 Dec 23;92:605–607. [PubMed] [Google Scholar]
  7. PETERSON W. J., EVANS W. R., LECCE E., BELL T. A., ETCHELLS J. L. Quantitative determination of the carotenoids in yeasts of the genus Rhodotorula. J Bacteriol. 1958 May;75(5):586–591. doi: 10.1128/jb.75.5.586-591.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. THYAGARAJAN T. R., CONTI S. F., NAYLOR H. B. Electron microscopy of yeast mitochondria. Exp Cell Res. 1961 Oct;25:216–218. doi: 10.1016/0014-4827(61)90331-7. [DOI] [PubMed] [Google Scholar]

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