Abstract
A culture of Chlorella established by 30-fold dilution of a culture already grown to a level of 15 ml packed cell volume per liter produces little chlorophyll for approximately 12 hours. Investigation of other characteristics such as nitrogen incorporation, increase in packed cell volume and dry weight as well as RNA level show all of these to increase without any significant lag. α-Linolenate, which can be considered as a chloroplast marker, increased markedly. Photosynthetic oxygen evolution and respiration as well as the heme enzyme, catalase, increase also, indicating that the lag in chlorophyll synthesis is not due to a general inability to produce the porphyrin moiety.
The lag period was demonstrated not to be related to a deficiency of culture elements nor to inhibitors produced. Neither was there a need for a conditioning period of the medium.
It is concluded that the lag in chlorophyll synthesis is unique and that the reasons for it are related to the response of the cells to environmental conditions following dilution.
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Selected References
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- Bloch K. E., Chano S. B. Galactolipids and Photosynthetic Oxygen Evolution. Science. 1964 May 1;144(3618):560–560. doi: 10.1126/science.144.3618.560-a. [DOI] [PubMed] [Google Scholar]
- CLAYTON R. K. Permeability barriers and the assay of catalase in intact cells. Biochim Biophys Acta. 1959 Nov;36:35–39. doi: 10.1016/0006-3002(59)90066-6. [DOI] [PubMed] [Google Scholar]
- EAGLE H., PIEZ K. A., OYAMA V. I. The biosynthesis of cystine in human cell cultures. J Biol Chem. 1961 May;236:1425–1428. [PubMed] [Google Scholar]
- ERWIN J., BLOCH K. BIOSYNTHESIS OF UNSATURATED FATTY ACIDS IN MICROORGANISMS. Science. 1964 Mar 6;143(3610):1006–1012. doi: 10.1126/science.143.3610.1006. [DOI] [PubMed] [Google Scholar]
- ERWIN J., BLOCH K. POLYUNSATURATED FATTY ACIDS IN SOME PHOTOSYNTHETIC MICROORGANISMS. Biochem Z. 1963;338:496–511. [PubMed] [Google Scholar]
- HASE E., MORIMURA Y., TAMIYA H. Some data on the growth physiology of Chlorella studied by the technique of synchronous culture. Arch Biochem Biophys. 1957 Jul;69:149–165. doi: 10.1016/0003-9861(57)90482-4. [DOI] [PubMed] [Google Scholar]
- Shugarman P. M., Appleman D. Natural Variations in the Physiological Characteristics of Growing Chlorella Cultures. Plant Physiol. 1965 Jan;40(1):81–84. doi: 10.1104/pp.40.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]