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. 1979 Jan;23(1):94–98. doi: 10.1128/iai.23.1.94-98.1979

Dissociation of leukocyte alkaline phosphatase from the bactericidal activity of neutrophils.

L R DeChatelet, P S Shirley, C E McCall, D A Bass
PMCID: PMC550694  PMID: 370011

Abstract

We have identified an apparently healthy individual with no history of repeated infections whose neutrophils were virtually devoid of alkaline phosphatase activity by either spectrophotometric or histochemical assay. His cells showed normal bactericidal activity toward both Staphylococcus aureus and Escherichia coli when tested in vitro. We have also demonstrated that L-p-bromolevamisole, a potent inhibitor of alkaline phosphatase in many tissues, is likewise an effective inhibitor of the neutrophilic enzyme. Concentrations of this compound which caused nearly complete inhibition of the neutrophilic enzyme did not impair the ability of intact cells to kill bacteria. These results suggest that leukocyte alkaline phosphatase is not required for the normal bactericidal activity of neutrophils.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bass D. A., DeChatelet L. R., Burk R. F., Shirley P., Szejda P. Polymorphonuclear leukocyte bactericidal activity and oxidative metabolism during glutathione peroxidase deficiency. Infect Immun. 1977 Oct;18(1):78–84. doi: 10.1128/iai.18.1.78-84.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DeChatelet L. R., Cooper M. R. A modified procedure for the determination of leukocyte alkaline phosphatase. Biochem Med. 1970 Aug;4(1):61–68. doi: 10.1016/0006-2944(70)90103-1. [DOI] [PubMed] [Google Scholar]
  3. DeChatelet L. R., Shirley P. S., Johnston R. B., Jr Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood. 1976 Apr;47(4):545–554. [PubMed] [Google Scholar]
  4. Fishman W. H. Perspectives on alkaline phosphatase isoenzymes. Am J Med. 1974 May;56(5):617–650. doi: 10.1016/0002-9343(74)90631-7. [DOI] [PubMed] [Google Scholar]
  5. Gorodischer R., Davidson R. G., Mosovich L. L., Yaffe S. J. Hypophosphatasia: a developmental anomaly of alkaline phosphatase? Pediatr Res. 1976 Jul;10(7):650–656. doi: 10.1203/00006450-197607000-00004. [DOI] [PubMed] [Google Scholar]
  6. KAPLOW L. S. A histochemical procedure for localizing and evaluating leukocyte alkaline phosphatase activity in smears of blood and marrow. Blood. 1955 Oct;10(10):1023–1029. [PubMed] [Google Scholar]
  7. Koch C. Acquired defect in the bactericidal function of neutrophil granulocytes during bacterial infections. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):439–447. doi: 10.1111/j.1699-0463.1974.tb02348.x. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. McCall C. E., Caves J., Cooper R., DeChatlet L. Functional characteristics of human toxic neutrophils. J Infect Dis. 1971 Jul;124(1):68–75. doi: 10.1093/infdis/124.1.68. [DOI] [PubMed] [Google Scholar]
  10. McCall C. E., Katayama I., Cotran R. S., Finland M. Lysosomal and ultrastructural changes in human "toxic" neutrophils during bacterial infection. J Exp Med. 1969 Feb 1;129(2):267–293. doi: 10.1084/jem.129.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Qualliotine D., DeChatelet L. R., McCall C. E., Cooper M. R. Effect of catecholamines on the bactericidal activity of polymorphonuclear leukocytes. Infect Immun. 1972 Sep;6(3):211–217. doi: 10.1128/iai.6.3.211-217.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rausch P. G., Moore T. G. Granule enzymes of polymorphonuclear neutrophils: A phylogenetic comparison. Blood. 1975 Dec;46(6):913–919. [PubMed] [Google Scholar]
  13. Repine J. E., Clawson C. C., Brunning R. D. Primary leucocyte alkaline phosphatase deficiency in an adult with repeated infections. Br J Haematol. 1976 Sep;34(1):87–94. doi: 10.1111/j.1365-2141.1976.tb00177.x. [DOI] [PubMed] [Google Scholar]
  14. Strauss R. G., Bove K. E., Jones J. F., Mauer A. M., Fulginiti V. A. An anomaly of neutrophil morphology with impaired function. N Engl J Med. 1974 Feb 28;290(9):478–484. doi: 10.1056/NEJM197402282900903. [DOI] [PubMed] [Google Scholar]
  15. Van Belle H., De Broe M. E., Wieme R. J. L-p-Bromotetramisole, a new reagent for use in measuring placental or intestinal isoenzymes of alkaline phosphatase in human serum. Clin Chem. 1977 Mar;23(3):454–459. [PubMed] [Google Scholar]

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