Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Jan;27(1):38–43. doi: 10.1128/iai.27.1.38-43.1980

Immunization of mice with Trypanosoma cruzi polyribosomes.

L L Leon, W Leon, L Chaves, S C Costa, M Q Cruz, H M Brascher, A O Lima
PMCID: PMC550718  PMID: 6987175

Abstract

Studies were carried out with a polyribosomal fraction isolated from Trypanosoma cruzi Y epimastigotes, with the intention to determine both its immunogenic activity and the degree of protection it could induce against experimental T. cruzi infection. This fraction was assayed in four groups of mice by using different schedules of vaccination and varying the dose, intervals, and route of administration. Seven days after the last dose, the animals were sacrificed for immunological studies or subjected to challenge with T. cruzi trypomastigotes. The results obtained in all schedules showed that our polyribosomal fraction only induced a weak antibody response, but was capable of evoking an expressive cellular response. It was also shown that this fraction has the capacity of inducing a high degree of protection against T. cruzi infection, as determined by the decrease of parasitemia and the prolonged survival time of immunized animals.

Full text

PDF
38

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamsohn I. A., Silva W. D. Antibody dependent cell-mediated cytotoxicity against Trypanosoma cruzi. Parasitology. 1977 Dec;75(3):317–323. doi: 10.1017/s0031182000051866. [DOI] [PubMed] [Google Scholar]
  2. Angerman C. R., Eisenstein T. K. Comparative efficacy and toxicity of a ribosomal vaccine, acetone-killed cells, lipopolysaccharide, and a live cell vaccine prepared from Salmonella typhhimurium. Infect Immun. 1978 Feb;19(2):575–582. doi: 10.1128/iai.19.2.575-582.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avrameas S., Taudou B., Chuilon S. Glutaraldehyde, cyanuric chloride and tetrazotized O-dianisidine as coupling reagents in the passive hemagglutination test. Immunochemistry. 1969 Jan;6(1):67–76. doi: 10.1016/0019-2791(69)90179-7. [DOI] [PubMed] [Google Scholar]
  4. Boshes R. A. Drosophila polyribosomes. The characterization of two populations by cell fractionation and isotopic labeling with nucleic acid and protein precursors. J Cell Biol. 1970 Sep;46(3):477–490. doi: 10.1083/jcb.46.3.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clinton B. A., Ortiz-Ortiz L., Garcia W., Martinez T., Capin R. Trypanosoma cruzi: early immune responses in infected mice. Exp Parasitol. 1975 Jun;37(3):417–425. doi: 10.1016/0014-4894(75)90012-0. [DOI] [PubMed] [Google Scholar]
  6. Corsini A. C., Clayton C., Askonas B. A., Ogilvie B. M. Suppressor cells and loss of B-cell potential in mice infected with Trypanosoma brucei. Clin Exp Immunol. 1977 Jul;29(1):122–131. [PMC free article] [PubMed] [Google Scholar]
  7. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  8. Dos Santos R. B., De Oliveira J. C. Letter: Antibodies to neurons in chronic Chagas' disease. Trans R Soc Trop Med Hyg. 1976;70(2):167–167. doi: 10.1016/0035-9203(76)90195-4. [DOI] [PubMed] [Google Scholar]
  9. Eisenstein T. K. Evidence for O antigens as the antigenic determinants in "ribosomal" vaccines prepared from Salmonella. Infect Immun. 1975 Aug;12(2):364–377. doi: 10.1128/iai.12.2.364-377.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feit C., Tewari R. P. Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum. Infect Immun. 1974 Nov;10(5):1091–1097. doi: 10.1128/iai.10.5.1091-1097.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grymberg N., Guimaraes F. N., de Castro F. T., Lima A. O. Effect of the injection of ribosomes and RNA from Crithidia fasciculata on the experimental infection of mice by Trypanosoma cruzi. Trans R Soc Trop Med Hyg. 1977;71(1):35–36. doi: 10.1016/0035-9203(77)90204-8. [DOI] [PubMed] [Google Scholar]
  12. Hoops P., Prather N. E., Berry J., Ravel J. M. Evidence for an extrinsic immunogen in effective ribosomal vaccines from Salmonella typhimurium. Infect Immun. 1976 Apr;13(4):1184–1192. doi: 10.1128/iai.13.4.1184-1192.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jayawardena A. N., Waksman B. H., Eardley D. D. Activation of distinct helper and suppressor T cells in experimental trypanosomiasis. J Immunol. 1978 Aug;121(2):622–628. [PubMed] [Google Scholar]
  14. Johnson W. Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium. Infect Immun. 1973 Sep;8(3):395–400. doi: 10.1128/iai.8.3.395-400.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kierszenbaum F., Weinman D. Antibody-independent activation of the alternative complement pathway in human serum by parasitic cells. Immunology. 1977 Feb;32(2):245–249. [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Leon W., Fouts D. L., Manning J. Sequence arrangement of the 16S and 26S rRNA genes in the pathogenic haemoflagellate Leishmania donovani. Nucleic Acids Res. 1978 Feb;5(2):491–504. doi: 10.1093/nar/5.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moorhead J. W. Tolerance and contact sensitivity to DNFA in mice. VIII. Identification of distinct T cell subpopulations that mediate in vivo and in vitro manifestations of delayed hypersensitivity. J Immunol. 1978 Jan;120(1):137–144. [PubMed] [Google Scholar]
  20. Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
  21. Preston P. M., Dumonde D. C. Immunogenicity of a ribosomal antigen of Leishmania enriettii. Trans R Soc Trop Med Hyg. 1971;65(1):18–19. [PubMed] [Google Scholar]
  22. Reed S. G., Larson C. L., Speer C. A. Suppression of cell-mediated immunity in experimental Chagas' disease. Z Parasitenkd. 1977 Jun 3;52(1):11–17. doi: 10.1007/BF00380553. [DOI] [PubMed] [Google Scholar]
  23. Santos-Buch C. A., Teixeira A. R. The immunology of experimental Chagas' disease. 3. Rejection of allogeneic heart cells in vitro. J Exp Med. 1974 Jul 1;140(1):38–53. doi: 10.1084/jem.140.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schmuñis G. A., Szarfman A., Pesce U. J., González-Cappa S. M. The effect of acute infection by Trypanosoma cruzi upon the response of mice to sheep erythrocytes. Rev Inst Med Trop Sao Paulo. 1977 Sep-Oct;19(5):323–331. [PubMed] [Google Scholar]
  25. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Venneman M. R. Purification of immunogenically active ribonucleic acid preparations of Salmonella typhimurium: molecular-sieve and anion-exchange chromatography. Infect Immun. 1972 Mar;5(3):269–282. doi: 10.1128/iai.5.3.269-282.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WILLIAMS J. S., DUXBURY R. E., ANDERSON R. I., SADUN E. H. Fluorescent antibody reactions in Trypanosoma rhodesiense and T. gambiense in experimental animals. J Parasitol. 1963 Jun;49:380–384. [PubMed] [Google Scholar]
  28. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Youmans A. S., Youmans G. P. Preparation of highly immunogenic ribosomal fractions of Mycobacterium tuberculosis by use of sodium dodecyl sulfate. J Bacteriol. 1966 Jun;91(6):2139–2145. doi: 10.1128/jb.91.6.2139-2145.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Youmans A. S., Youmans G. P. The effect of metabolic inhibitors and hydroxylamine on the immune response in mice to mycobacterial ribonucleic acid vaccines. J Immunol. 1974 Jan;112(1):271–284. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES