Abstract
Proteolysis by four strains of Legionella pneumophila, the etiological agent of Legionnaires disease, was studied by the method of immunoelectrophoresis. Twenty-three human serum proteins were tested as substrates. Five proteins were degraded: alpha 1-acid glycoprotein, alpha 1-antichymotrypsin, beta-lipoprotein, beta 1E-globulin, and beta 2-glycoprotein-I. Moreover, the degradation of alpha 1-antichymotrypsin was demonstrated by investigation of an enzyme-blocking test. It is suggested that the proteolytic activity of L. pneumophila may bear some relationship to its pathogenic activity.
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- Baine W. B., Rasheed J. K., Mackel D. C., Bopp C. A., Wells J. G., Kaufmann A. F. Exotoxin activity associated with the Legionnaires disease bacterium. J Clin Microbiol. 1979 Mar;9(3):453–456. doi: 10.1128/jcm.9.3.453-456.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feeley J. C., Gorman G. W., Weaver R. E., Mackel D. C., Smith H. W. Primary isolation media for Legionnaires disease bacterium. J Clin Microbiol. 1978 Sep;8(3):320–325. doi: 10.1128/jcm.8.3.320-325.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. Cellular fatty acid composition of isolates from Legionnaires disease. J Clin Microbiol. 1977 Aug;6(2):140–143. doi: 10.1128/jcm.6.2.140-143.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Müller H. E. Immunelektrophoretisch nachweisbare Liquorproteinveränderungen bei bakteriellen Meningitiden. Pathol Microbiol (Basel) 1972;38(2):83–92. [PubMed] [Google Scholar]
- Müller H. E., Marklein G. Untersuchungen zur proteolytischen Enzymaktivität bei Bacillus anthracis und Bacillus cereus. Pathol Microbiol (Basel) 1973;39(5):364–372. [PubMed] [Google Scholar]
- Müller H. E. Methodische Untersuchungen in vitro zur Einwirkung bakterieller Proteasen auf menschliche Plasmaeiweisskörper, dargestellt am Beispiel von Pseudomonas aeruginosa. Zentralbl Bakteriol Orig. 1971;216(1):79–87. [PubMed] [Google Scholar]
- Müller H. E. Neuraminidase activity in Streptococcus sanguis and in the viridans group, and occurrence of acylneuraminate lyase in viridans organisms isolated from patients with septicemia. Infect Immun. 1974 Feb;9(2):323–328. doi: 10.1128/iai.9.2.323-328.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Müller H. E., Sethi K. K. Proteolytic activity of Cryptococcus neoformans against human plasma proteins. Med Microbiol Immunol. 1972;158(2):129–134. doi: 10.1007/BF02120478. [DOI] [PubMed] [Google Scholar]
- SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
- Schallehn G., Müller H. E. Uber die Einwirkung proteolytischer Enzyme von Clostridium histolyticum und Clostridium novyi auf menschliche Plasmaeiweibbkörper in Wachstumskulturen. Zentralbl Bakteriol Orig A. 1973 Jun;224(1):102–114. [PubMed] [Google Scholar]
- Sethi K. K., Müller H. E. Immunoelectrophoretic demonstration of proteolytic activity against human proteins by Mycoplasma laidlawii. Z Immunitatsforsch Allerg Klin Immunol. 1970 Aug;140(2):184–188. [PubMed] [Google Scholar]
- Werner H., Müller H. E. Immunelektrophoretische Untersuchungen über die Einwirkung von Bacteroides-, Fusobacterium-, Leptotrichia- und Sphaerophorus-Arten auf menschliche Plasmaproteine. Zentralbl Bakteriol Orig. 1971;216(1):96–113. [PubMed] [Google Scholar]