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. 1980 Jan;27(1):204–210. doi: 10.1128/iai.27.1.204-210.1980

Ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa.

R Gonggrijp, W J Mullers, P J Lemmens, C P van Boven
PMCID: PMC550745  PMID: 6153637

Abstract

In mice, active protection against Pseudomonas aeruginosa could be induced with two fractions derived from a crude preparation of ribosomes from P. aeruginosa. The two fractions were obtained by gel filtration chromatography of the crude ribosomal preparation on Sepharose CL-2B. In fraction I, less than 1% of the ribonucleic acid (RNA) applied to the column was recovered. Fraction II contained RNA and protein in a ratio of 1.94. The presence of ribosomes in this fraction was confirmed by analysis on a sucrose density gradient. The protection by fraction I was not affected by treatment with ribonuclease; in contrast, incubation of fraction II with ribonuclease completely abolished active protection. Fraction I contained lipopolysaccharide (LPS) as was indicated by the presence of 2-keto-3-deoxyoctonic acid. No LPS was found in fraction II. The adjuvant dimethyl dioctadecyl ammonium bromide enhanced the protection by fraction II; however, immunity by a low dose of fraction I was abolished by dimethyl dioctadecyl ammonium bromide. Protection by fractions I and II appeared to be restricted to the homologous serotype of P. aeruginosa. These results indicate that RNA is required for protection by fraction II. Active protection by fraction I is likely due to LPS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andron LA I. I., Eigelsbach H. T. Biochemical and immunological properties of ribonucleic acid-rich extracts from Francisella tularensis. Infect Immun. 1975 Jul;12(1):137–142. doi: 10.1128/iai.12.1.137-142.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Angerman C. R., Eisenstein T. K. Comparative efficacy and toxicity of a ribosomal vaccine, acetone-killed cells, lipopolysaccharide, and a live cell vaccine prepared from Salmonella typhhimurium. Infect Immun. 1978 Feb;19(2):575–582. doi: 10.1128/iai.19.2.575-582.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feit C., Tewari R. P. Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum. Infect Immun. 1974 Nov;10(5):1091–1097. doi: 10.1128/iai.10.5.1091-1097.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
  6. Horton D., Rodemeyer G., Haskell T. H. Analytical characterization of lipopolysaccharide antigens from seven strains of Pseudomonas aeruginosa. Carbohydr Res. 1977 May;55:35–47. doi: 10.1016/s0008-6215(00)84441-9. [DOI] [PubMed] [Google Scholar]
  7. Jensen R., Gregory B., Naylor J., Actor P. Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations. Infect Immun. 1972 Aug;6(2):156–161. doi: 10.1128/iai.6.2.156-161.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lieberman M. M., McKissock D. C., Wright G. L. Passive immunization against Pseudomonas with a ribosomal vaccine-induced immune serum and immunoglobulin fractions. Infect Immun. 1979 Feb;23(2):509–521. doi: 10.1128/iai.23.2.509-521.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lieberman M. M. Pseudomonas ribosomal vaccines: preparation, properties, and immunogenicity. Infect Immun. 1978 Jul;21(1):76–86. doi: 10.1128/iai.21.1.76-86.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pennington J. E. Preliminary investigations of Pseudomonas aeruginosa vaccine in patients with leukemia and cystic fibrosis. J Infect Dis. 1974 Nov;130 (Suppl)(0):S159–S162. doi: 10.1093/infdis/130.supplement.s159. [DOI] [PubMed] [Google Scholar]
  11. Pier G. B., Sidberry H. F., Sadoff J. C. Protective immunity induced in mice by immunization with high-molecular-weight polysaccharide from Pseudomonas aeruginosa. Infect Immun. 1978 Dec;22(3):919–925. doi: 10.1128/iai.22.3.919-925.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. REED C. F., SWISHER S. N., MARINETTI G. V., ENEN E. G. Studies of the lipids of the erythrocyte. I. Quantitative analysis of the lipids of normal human red blood cells. J Lab Clin Med. 1960 Aug;56:281–289. [PubMed] [Google Scholar]
  13. Smith R. L., Wysocki J. A., Bruun J. N., De Courcy S. J., Jr, Blakemore W. S., Mudd S. Efficacy of ribosomal preparations from Pseudomonas aeruginosa to protect against intravenous Pseudomonas challenge in mice. J Reticuloendothel Soc. 1974 Jan;15(1):22–30. [PubMed] [Google Scholar]
  14. Swendsen C. L., Johnson W. Humoral immunity to Streptococcus pneumoniae induced by a pneumococcal ribosomal protein fraction. Infect Immun. 1976 Aug;14(2):345–354. doi: 10.1128/iai.14.2.345-354.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
  16. Wesley J., Fisher A., Fisher M. W. Immunization against Pseudomonas in infection after thermal injury. J Infect Dis. 1974 Nov;130 (Suppl)(0):S152–S158. doi: 10.1093/infdis/130.supplement.s152. [DOI] [PubMed] [Google Scholar]
  17. Willers J. M., Bloksma N., van der Meer C., Snippe H., van Dijk H., de Reuver M. J., Hofhuis F. M. Regulation of the immune response by macrophages. Antonie Van Leeuwenhoek. 1979;45(1):41–48. doi: 10.1007/BF00400777. [DOI] [PubMed] [Google Scholar]
  18. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Youmans A. S., Youmans G. P. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis. J Bacteriol. 1966 Jun;91(6):2146–2154. doi: 10.1128/jb.91.6.2146-2154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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