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. 1980 Jan;27(1):222–230. doi: 10.1128/iai.27.1.222-230.1980

Prevalence of Pilus Antigens, Enterotoxin Types, and Enteropathogenicity Among K88-Negative Enterotoxigenic Escherichia coli from Neonatal Pigs

H W Moon 1, E M Kohler 2, R A Schneider 1, S C Whipp 1
PMCID: PMC550748  PMID: 6102079

Abstract

Enterotoxigenic Escherichia coli (ETEC) that were isolated from neonatal pigs and that did not react in preliminary tests for pilus antigen K88 were subjected to additional tests for K88 and for pilus antigens K99 and 987P. Four such isolates produced K88, 9 isolates produced K99, 55 isolates produced 987P, and the remaining 43 isolates produced none of the three pilus antigens (3P−). Immunofluorescence tests of ileal sections from pigs were more sensitive for 987P detection than was serum agglutination of bacteria grown from the ileum. Most ETEC that produced K88, K99, or 987P were enteropathogenic (adhered to ileal villi, colonized intensively, and caused profuse diarrhea) when given to neonatal pigs. In contrast, only 3 of the 43 ETEC that produced none of the pilus antigens were enteropathogenic. The isolates were also tested for the type of enterotoxin produced. The K88+ isolates all produced heat-labile enterotoxin (LT) detectable in cultured adrenal cells (i.e., were LT+). None of the 987P+, K99+, or enterpathogenic 3P− isolates produced LT. However (except for a single K99+ isolate), they all produced heat-stable enterotoxin detectable in infant mice (STa+). Sixteen isolates produced neither LT nor STa but did produce enterotoxin detectable in ligated intestinal loops of pigs (STb). Most of these LT− STa− STb+ isolates were also K88−, K99−, and 987P− and non-enteropathogenic. One of them was K99+ and enteropathogenic. Our conclusions are as follows. (i) Most enteropathogenic ETEC from neonatal pigs produce either K88, 987P, or K99; however, there are some that produce none of the three antigens. (ii) Immunofluorescence tests for pilus antigens produced in vivo are recommended for the diagnosis of ETEC infections. (iii) Reports of LT− STa− STb+ swine ETEC are confirmed; furthermore, such isolates can be enteropathogenic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acres S. D., Isaacson R. E., Babiuk L. A., Kapitany R. A. Immunization of calves against enterotoxigenic colibacillosis by vaccinating dams with purified K99 antigen and whole cell bacterins. Infect Immun. 1979 Jul;25(1):121–126. doi: 10.1128/iai.25.1.121-126.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alderete J. F., Robertson D. C. Nutrition and enterotoxin synthesis by enterotoxigenic strains of Escherichia coli: defined medium for production of heat-stable enterotoxin. Infect Immun. 1977 Mar;15(3):781–788. doi: 10.1128/iai.15.3.781-788.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burgess M. N., Bywater R. J., Cowley C. M., Mullan N. A., Newsome P. M. Biological evaluation of a methanol-soluble, heat-stable Escherichia coli enterotoxin in infant mice, pigs, rabbits, and calves. Infect Immun. 1978 Aug;21(2):526–531. doi: 10.1128/iai.21.2.526-531.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  5. Guinée P. A., Agterberg C. M., Jansen W. H., Frik J. F. Serological identification of pig enterotoxigenic Escherichia coli strains not belonging to the classical serotypes. Infect Immun. 1977 Feb;15(2):549–555. doi: 10.1128/iai.15.2.549-555.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guinée P. A., Veldkamp J., Jansen W. H. Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infect Immun. 1977 Feb;15(2):676–678. doi: 10.1128/iai.15.2.676-678.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gyles C. L., Stevens J. B., Craven J. A. A study of Escherichia coli strains isolated from pigs with gastro-intestinal disease. Can J Comp Med. 1971 Jul;35(3):258–266. [PMC free article] [PubMed] [Google Scholar]
  8. Hadad J. J., Gyles C. L. Detection of bovine enteropathogenic Escherichia coli by indirect fluorescent antibody technique. Am J Vet Res. 1978 Oct;39(10):1651–1655. [PubMed] [Google Scholar]
  9. Isaacson R. E., Moon H. W., Schneider R. A. Distribution and virulence of Escherichia coli in the small intestines of calves with and without diarrhea. Am J Vet Res. 1978 Nov;39(11):1750–1755. [PubMed] [Google Scholar]
  10. Larivière S., Lallier R., Morin M. Evaluation of various methods for the detection of enteropathogenic Escherichia coli in diarrheic calves. Am J Vet Res. 1979 Jan;40(1):130–134. [PubMed] [Google Scholar]
  11. Moon H. W., Fung P. Y., Isaacson R. E., Booth G. D. Effects of age, ambient temperature, and heat-stable Escherichia coli enterotoxin on intestinal transit in infant mice. Infect Immun. 1979 Jul;25(1):127–132. doi: 10.1128/iai.25.1.127-132.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moon H. W., Fung P. Y., Whipp S. C., Isaacson R. E. Effects of age and ambient temperature on the responses of infant mice to heat-stable enterotoxin of Escherichia coli: assay modifications. Infect Immun. 1978 Apr;20(1):36–39. doi: 10.1128/iai.20.1.36-39.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moon H. W., Isaacson R. E., Pohlenz J. Mechanisms of association of enteropathogenic Escherichia coli with intestinal epithelium. Am J Clin Nutr. 1979 Jan;32(1):119–127. doi: 10.1093/ajcn/32.1.119. [DOI] [PubMed] [Google Scholar]
  14. Moon H. W., Nagy B., Isaacson R. E., Orskov I. Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs. Infect Immun. 1977 Feb;15(2):614–620. doi: 10.1128/iai.15.2.614-620.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morgan R. L., Isaacson R. E., Moon H. W., Brinton C. C., To C. C. Immunization of suckling pigs against enterotoxigenic Escherichia coli-induced diarrheal disease by vaccinating dams with purified 987 or K99 pili: protection correlates with pilus homology of vaccine and challenge. Infect Immun. 1978 Dec;22(3):771–777. doi: 10.1128/iai.22.3.771-777.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nagy B., Moon H. W., Isaacson R. E. Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infect Immun. 1977 Apr;16(1):344–352. doi: 10.1128/iai.16.1.344-352.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nagy B., Moon H. W., Isaacson R. E. Colonization of porcine small intestine by Escherichia coli: ileal colonization and adhesion by pig enteropathogens that lack K88 antigen and by some acapsular mutants. Infect Immun. 1976 Apr;13(4):1214–1220. doi: 10.1128/iai.13.4.1214-1220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nagy B., Moon H. W., Isaacson R. E., To C. C., Brinton C. C. Immunization of suckling pigs against enteric enterotoxigenic Escherichia coli infection by vaccinating dams with purified pili. Infect Immun. 1978 Jul;21(1):269–274. doi: 10.1128/iai.21.1.269-274.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Orskov I., Orskov F., Smith H. W., Sojka W. J. The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called "Kco", possessed by calf and lamb enteropathogenic strains. Acta Pathol Microbiol Scand B. 1975 Feb;83(1):31–36. doi: 10.1111/j.1699-0463.1975.tb00066.x. [DOI] [PubMed] [Google Scholar]
  20. Rutter J. M., Jones G. W., Brown G. T., Burrows M. R., Luther P. D. Antibacterial activity in colostrum and milk associated with protection of piglets against enteric disease caused by K88-positive Escherichia coli. Infect Immun. 1976 Mar;13(3):667–676. doi: 10.1128/iai.13.3.667-676.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith H. W., Gyles C. L. The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. J Med Microbiol. 1970 Aug;3(3):387–401. doi: 10.1099/00222615-3-3-387. [DOI] [PubMed] [Google Scholar]
  22. Smith H. W., Huggins M. B. The influence of plasmid-determined and other characteristics of enteropathogenic Escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs. J Med Microbiol. 1978 Nov;11(4):471–492. doi: 10.1099/00222615-11-4-471. [DOI] [PubMed] [Google Scholar]
  23. Smith H. W., Linggood M. A. Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. J Med Microbiol. 1971 Nov;4(4):467–485. doi: 10.1099/00222615-4-4-467. [DOI] [PubMed] [Google Scholar]
  24. Sojka W. J., Wray C., Morris J. A. Passive protection of lambs against experimental enteric colibacillosis by colostral transfer of antibodies from K99-vaccinated ewes. J Med Microbiol. 1978 Nov;11(4):493–499. doi: 10.1099/00222615-11-4-493. [DOI] [PubMed] [Google Scholar]
  25. Söderlind O., Möllby R. Enterotoxins, O-groups, and K88 antigen in Escherichia coli from neonatal piglets with and without diarrhea. Infect Immun. 1979 Jun;24(3):611–616. doi: 10.1128/iai.24.3.611-616.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Whipp S. C., Moon H. W. Modification of enterosorption in experimental enteric colibacillosis of swine inoculated with Escherichia coli. J Infect Dis. 1973 Mar;127(3):255–260. doi: 10.1093/infdis/127.3.255. [DOI] [PubMed] [Google Scholar]

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