Abstract
An enzyme-linked immunosorbent assay has been developed to detect serum immunoglobulin G and M antibodies against pneumococcal polysaccharide antigens. Parameters affecting the specificity and sensitivity of the assay are described. A vigorous antibody response involving both the immunoglobulin G and M classes was demonstrated after pneumococcal polysaccharide immunization in normal adults. Studies with this enzyme-linked immunosorbent assay technique will allow further understanding of the biology of the primary and secondary immune response to Streptococcus pneumoniae in normals as well as in those persons most susceptible to infection with that organism.
Full text
PDF![411](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/9e1559a9a7bf/iai00170-0129.png)
![412](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/686dcb2122b7/iai00170-0130.png)
![413](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/da810b2b83ee/iai00170-0131.png)
![414](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/2cdce7a78a28/iai00170-0132.png)
![415](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/8fa72a32b1d6/iai00170-0133.png)
![416](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/c60486905cdc/iai00170-0134.png)
![417](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/956e/550780/d40d96831981/iai00170-0135.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ahlstedt S., Holmgren J., Hanson L. A. Protective capacity of antibodies against E. coli O antigen with special reference to the avidity. Int Arch Allergy Appl Immunol. 1974;46(3):470–480. doi: 10.1159/000231150. [DOI] [PubMed] [Google Scholar]
- Ammann A. J., Pelger R. J. Determination of antibody to pneumococcal polysaccharides with chromic chloride-treated human red blood cells and indirect hemagglutination. Appl Microbiol. 1972 Nov;24(5):679–683. doi: 10.1128/am.24.5.679-683.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Appelbaum P. C., Bhamjee A., Scragg J. N., Hallett A. F., Bowen A. J., Cooper R. C. Streptococcus pneumoniae resistant to penicillin and chloramphenicol. Lancet. 1977 Nov 12;2(8046):995–997. doi: 10.1016/s0140-6736(77)92892-6. [DOI] [PubMed] [Google Scholar]
- Austrian R. Pneumococcal infection and pneumococcal vaccine. N Engl J Med. 1977 Oct 27;297(17):938–939. doi: 10.1056/NEJM197710272971712. [DOI] [PubMed] [Google Scholar]
- Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
- Butler J. E., Feldbush T. L., McGivern P. L., Stewart N. The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity. Immunochemistry. 1978 Feb;15(2):131–136. doi: 10.1016/0161-5890(78)90053-6. [DOI] [PubMed] [Google Scholar]
- Engvall E., Jonsson K., Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta. 1971 Dec 28;251(3):427–434. doi: 10.1016/0005-2795(71)90132-2. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Granfors K. Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease. J Clin Microbiol. 1979 Mar;9(3):336–341. doi: 10.1128/jcm.9.3.336-341.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keren D. F. Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens. Infect Immun. 1979 May;24(2):441–448. doi: 10.1128/iai.24.2.441-448.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leinikki P. O., Shekarchi I., Dorsett P., Sever J. L. Determination of virus-specific IgM antibodies by using ELISA: elimination of false-positive results with protein A-Sepharose absorption and subsequent IgM antibody assay. J Lab Clin Med. 1978 Dec;92(6):849–857. [PubMed] [Google Scholar]
- Weits J., de Gast G. C., The T. H., Esselink M. T., Deelder A. M., Petrovic M., Mandema E. Class-specific antibody titres (ELISA) against the primary immunogen Helix pomatia haemocyanin (HPH) in man. Clin Exp Immunol. 1978 Jun;32(3):443–450. [PMC free article] [PubMed] [Google Scholar]
- Wisdom G. B. Enzyme-immunoassay. Clin Chem. 1976 Aug;22(8):1243–1255. [PubMed] [Google Scholar]
- Yolken R. H., Wyatt R. G., Kim H. W., Kapikian A. Z., Chanock R. M. Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. Infect Immun. 1978 Feb;19(2):540–546. doi: 10.1128/iai.19.2.540-546.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]