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. 2017 Mar 20;69(4):699–710. doi: 10.1007/s10616-017-0078-3

Table 5.

Frequency of binucleated cells with micronucleus, Nuclear Division Index (NDI) and percentage of reduction in mutagenicity (%Red) obtained in CBMN assay performed with digoxin associated with MMC in different protocols of treatment on the HeLa cell line

Treatments Protocols
Pre-complexation Pre-treatment Simultaneous treatment Post-treatment
MNBN (x¯±SD) NDI (x¯±SD) %Red (%) MNBN (x¯±SD) NDI (x¯±SD) %Red (%) MNBN (x¯±SD) NDI (x¯±SD) %Red (%) MNBN (x¯±SD) NDI (x¯±SD) %Red (%)
PBS 8.0 ± 0.8* 1.5 ± 0.01 8.7 ± 1.2* 1.5 ± 0.02 6.7 ± 1.2* 1.5 ± 0.03 8.0 ± 1.6* 1.4 ± 0.01
MMC 39.7 ± 1.2 1.4 ± 0.01# 36.3 ± 1.2 1.4 ± 0.01# 39.0 ± 1.6 1.3 ± 0.01# 35.0 ± 1.4 1.4 ± 0.00#
0.7 + MMC 37.3 ± 0.9 1.3 ± 0.01# 38.0 ± 2.2 1.2 ± 0.03# 35.0 ± 1.4 1.2 ± 0.02# 34.7 ± 1.7 1.4 ± 0.01
1.5 + MMC 38.7 ± 1.2 1.4 ± 0.01# 27.7 ± 1.2* 1.4 ± 0.01 31.3 38.3 ± 1.2 1.5 ± 0.02 31.3 ± 2.0 1.4 ± 0.01
3.0 + MMC 23.0 ± 1.6* 1.5 ± 0.01 52.6 31.7 ± 1.7* 1.4 ± 0.01 34.0 22.7 ± 1.2* 1.5 ± 0.02 50.3 27.3 ± 0.9 1.4 ± 0.01

MNBN binucleated cells with micronucleus, x¯±SD mean ± standard deviation, MMC Mitomycin C (4.8 µM)

* Statistically different from group treated with mutagen MMC (p < 0.05)

#Statistically different from negative control (PBS) (p < 0.05)