Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Feb;27(2):444–448. doi: 10.1128/iai.27.2.444-448.1980

Growth characteristics of group A streptococci in a new chemically defined medium.

I van de Rijn, R E Kessler
PMCID: PMC550785  PMID: 6991416

Abstract

A new chemically defined medium for the growth of group A streptococci has been formulated. The advantages of this new medium over previously described defined media are: (i) rates of growth (i.e., doubling times) of 20 strains were comparable to the rates of growth in complex media; (ii) each strain grew to a higher culture density in the new defined medium than in complex media; (iii) transfer from complex media with small inocula was possible without any prior adaptation regimen; and (iv) the production of virulence factors (i.e., M protein and hyaluronic acid) and extracellular enzymes during growth in this new medium was comparable to that in complex media.

Full text

PDF
444

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amdur B. H., Szabo E. I., Socransky S. S. Presence of squalene in gram-positive bacteria. J Bacteriol. 1978 Jul;135(1):161–163. doi: 10.1128/jb.135.1.161-163.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernheimer A. W., Pappenheimer A. M. Factors Necessary for Massive Growth of Group A Hemolytic Streptococcus. J Bacteriol. 1942 Apr;43(4):481–494. doi: 10.1128/jb.43.4.481-494.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAVIES H. C., KARUSH F., RUDD J. H. EFFECT OF AMINO ACIDS ON STEADY-STATE GROWTH OF A GROUP A HEMOLYTIC STREPTOCOCCUS. J Bacteriol. 1965 Feb;89:421–427. doi: 10.1002/path.1700890156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davies H. C., Karush F., Rudd J. H. Synthesis of M protein by group A hemolytic streptococci in completely synthetic media during steady-state growth. J Bacteriol. 1968 Jan;95(1):162–168. doi: 10.1128/jb.95.1.162-168.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FOX E. N., KRAMPITZ L. O. Studies on the biosynthesis of the M-protein of group A hemolytic streptococci. J Bacteriol. 1956 Apr;71(4):454–461. doi: 10.1128/jb.71.4.454-461.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOX E. N. Peptide requirements for the synthesis of streptococcal proteins. J Biol Chem. 1961 Jan;236:166–171. [PubMed] [Google Scholar]
  7. GINSBURG I., GROSSOWICZ N. Group A hemolytic streptococci. I. A chemically defined medium for growth from small inocula. Proc Soc Exp Biol Med. 1957 Oct;96(1):108–112. doi: 10.3181/00379727-96-23406. [DOI] [PubMed] [Google Scholar]
  8. Husby G., van de Rijn I., Zabriskie J. B., Abdin Z. H., Williams R. C., Jr Antibodies reacting with cytoplasm of subthalamic and caudate nuclei neurons in chorea and acute rheumatic fever. J Exp Med. 1976 Oct 1;144(4):1094–1110. doi: 10.1084/jem.144.4.1094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MARKOWITZ A. S., LANGE C. F., Jr STREPTOCOCCAL RELATED GLOMERULONEPHRITIS. I. ISOLATION, IMMUNOCHEMISTRY AND COMPARATIVE CHEMISTRY OF SOLUBLE FRACTIONS FROM TYPE 12 NEPHRITOGENIC STREPTOCOCCI AND HUMAN GLOMERULI. J Immunol. 1964 Apr;92:565–575. [PubMed] [Google Scholar]
  10. MICKELSON M. N. CHEMICALLY DEFINED MEDIUM FOR GROWTH STREPTOCOCCUS PYOGENES. J Bacteriol. 1964 Jul;88:158–164. doi: 10.1128/jb.88.1.158-164.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McIlwain H., Fildes P., Gladstone G. P., Knight B. C. Glutamine and the growth of Streptococcus haemolyticus. Biochem J. 1939 Feb;33(2):223–229. doi: 10.1042/bj0330223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mickelson M. N., Slade H. D. Absence of type specific M antigen from group A streptococci grown in a chemically defined medium. J Bacteriol. 1964 May;87(5):1251–1251. doi: 10.1128/jb.87.5.1251-.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OGBURN C. A., HARRIS T. N., HARRIS S. Extracellular antigens in steady-state cultures of the hemolytic Streptococcus: production of proteinase at low pH. J Bacteriol. 1958 Aug;76(2):142–151. doi: 10.1128/jb.76.2.142-151.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SLADE H. D., KNOX G. A., SLAMP W. C. The amino acid nutrition of group A hemolytic Streptococci, with reference to the effect of glutathione on the cystine requirement. J Bacteriol. 1951 Nov;62(5):669–675. doi: 10.1128/jb.62.5.669-675.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SLADE H. D., SLAMP W. C. The requirement of ovalbumin for the growth of group A hemolytic Streptococcus in a synthetic medium. J Exp Med. 1955 Sep 1;102(3):291–305. doi: 10.1084/jem.102.3.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Terleckyj B., Willett N. P., Shockman G. D. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. doi: 10.1128/iai.11.4.649-655.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zabriskie J. B., Freimer E. H. An immunological relationship between the group. A streptococcus and mammalian muscle. J Exp Med. 1966 Oct 1;124(4):661–678. doi: 10.1084/jem.124.4.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van de Rijn I., Zabriskie J. B., McCarty M. Group A streptococcal antigens cross-reactive with myocardium. Purification of heart-reactive antibody and isolation and characterization of the streptococcal antigen. J Exp Med. 1977 Aug 1;146(2):579–599. doi: 10.1084/jem.146.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES