Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Feb;27(2):597–603. doi: 10.1128/iai.27.2.597-603.1980

Characterization of a flagellar sheath protein of Vibrio cholerae.

K W Hranitzky, A Mulholland, A D Larson, E R Eubanks, L T Hart
PMCID: PMC550806  PMID: 7380541

Abstract

A flagellar sheath protein of Vibrio cholerae CA401 (Inaba) was characterized. Purity of the preparation was indicated by a single band on polyacrylamide gel electrophoresis gels and on Ouchterlony plates prepared with antibody against crude sheath material. The sheath protein was composed of three polypeptides with minimal molecular weights of 61,500, 60,000, and 56,500. The presence of sheath protein on the flagellum as well as on the outer membrane of the cell was demonstrated by ferritin labeling experiments with antiserum. Sheath protein antibody reacted similarly in labeling experiments and agglutination tests with a classical Ogawa strain and two nonagglutinating V. cholerae isolates, indicating that the sheath protein may represent the common Vibrio H antigen. Antibody specific for lipopolysaccharide labeled the cell but not the sheathed flagellum, which demonstrated that the sheath is not a simple extension of the outer membrane of the cell.

Full text

PDF
597

Images in this article

599Fig. 1
on p.599
600Fig. 4
on p.600
600Fig. 5
on p.600
601Fig. 6
on p.601
601Fig. 7
on p.601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auzins I. A comparative assay of O-somatic antigen 5 of Salmonellae. Aust J Exp Biol Med Sci. 1968 Feb;46(1):93–105. doi: 10.1038/icb.1968.8. [DOI] [PubMed] [Google Scholar]
  2. Bhattacharyya F. K. The agglutination reactions of cholera vibrios. Jpn J Med Sci Biol. 1977 Oct;30(5):259–268. doi: 10.7883/yoken1952.30.259. [DOI] [PubMed] [Google Scholar]
  3. Braatz J. A., McIntire K. R. A rapid and effecient method for the isolation of proteins from polyacrylamide cells. Prep Biochem. 1977;7(6):495–509. doi: 10.1080/00327487708065516. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Eubanks E. R., Guentzel M. N., Berry L. J. Virulence factors involved in the intraperitoneal infection of adult mice with Vibrio cholerae. Infect Immun. 1976 Feb;13(2):457–463. doi: 10.1128/iai.13.2.457-463.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOLLETT E. A., GORDON J. AN ELECTRON MICROSCOPE STUDY OF VIBRIO FLAGELLA. J Gen Microbiol. 1963 Aug;32:235–239. doi: 10.1099/00221287-32-2-235. [DOI] [PubMed] [Google Scholar]
  7. GLAUERT A. M., KERRIDGE D., HORNE R. W. THE FINE STRUCTURE AND MODE OF ATTACHMENT OF THE SHEATHED FLAGELLUM OF VIBRIO METCHNIKOVII. J Cell Biol. 1963 Aug;18:327–336. doi: 10.1083/jcb.18.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guentzel M. N., Berry L. J. Motility as a virulence factor for Vibrio cholerae. Infect Immun. 1975 May;11(5):890–897. doi: 10.1128/iai.11.5.890-897.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LIU P. V. Survey of hemolysin production among species of pseudomonads. J Bacteriol. 1957 Dec;74(6):718–727. doi: 10.1128/jb.74.6.718-727.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Seidler R. J., Starr M. P. Structure of the flagellum of Bdellovibrio bacteriovorus. J Bacteriol. 1968 May;95(5):1952–1955. doi: 10.1128/jb.95.5.1952-1955.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith R. W., Koffler H. Bacterial flagella. Adv Microb Physiol. 1971;6:219–339. doi: 10.1016/s0065-2911(08)60070-3. [DOI] [PubMed] [Google Scholar]
  13. Wardi A. H., Michos G. A. Alcian blue staining of glycoproteins in acrylamide disc electrophoresis. Anal Biochem. 1972 Oct;49(2):607–609. doi: 10.1016/0003-2697(72)90472-1. [DOI] [PubMed] [Google Scholar]
  14. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  15. Yang G. C., Schrank G. D., Freeman B. A. Purification of flagellar cores of Vibrio cholerae. J Bacteriol. 1977 Feb;129(2):1121–1128. doi: 10.1128/jb.129.2.1121-1128.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES