Abstract
A new type of antibody-enzyme conjugate was made, and its possible application to Candida infection was studied. Both lactoperoxidase and xanthine oxidase were conjugated to specific antibody against Candida albicans. In vitro microbiocidal activity of the new antibody-enzyme conjugate, when incubated together with xanthine and minute amount of halides, showed a remarkable level of candidacidal ability. When the new antibody-enzyme conjugate was given to Candida-infected mice, followed by injecting xanthine and a minute amount of halides, about 50% of these heavily infected mice survived, whereas all control nontreated mice died. These data suggest that the further eleboration of this new antibody-enzyme conjugate might lead us to improve our therapeutic methods of clinical medicine.
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Selected References
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- Klebanoff S. J. Myeloperoxidase-halide-hydrogen peroxide antibacterial system. J Bacteriol. 1968 Jun;95(6):2131–2138. doi: 10.1128/jb.95.6.2131-2138.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knowles D. M., 2nd, Sulivan T. J., 3rd, Parker C. W., Williams R. C., Jr In vitro antibody-enzyme conjugates with specific bactericidal activity. J Clin Invest. 1973 Jun;52(6):1443–1452. doi: 10.1172/JCI107318. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Massey V., Brumby P. E., Komai H. Studies on milk xanthine oxidase. Some spectral and kinetic properties. J Biol Chem. 1969 Apr 10;244(7):1682–1691. [PubMed] [Google Scholar]
- Moolten F. L., Cooperband S. R. Selective destruction of target cells by diphtheria toxin conjugated to antibody directed against antigens on the cells. Science. 1970 Jul 3;169(3940):68–70. doi: 10.1126/science.169.3940.68. [DOI] [PubMed] [Google Scholar]
- Parker C. W., Aach R. D., Philpott G. W. Enzymatic activation and trapping of luminol-substituted peptides and proteins. A possible means of amplifying the cytotoxicity of anti-tumor antibodies. Proc Natl Acad Sci U S A. 1975 Jan;72(1):338–342. doi: 10.1073/pnas.72.1.338. [DOI] [PMC free article] [PubMed] [Google Scholar]