Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Mar;27(3):862–866. doi: 10.1128/iai.27.3.862-866.1980

Humoral and cellular immune responses of seronegative children vaccinated with a cold-adapted influenza A/HK/123/77 (H1N1) recombinant virus.

A Lazar, N Okabe, P F Wright
PMCID: PMC550853  PMID: 6155338

Abstract

Humoral and cell-mediated immune responses of young, seronegative children were assessed after intranasal vaccination with a cold-adapted influenza. A/HK/77 (H1N1) CR 35 recombinant virus. Vaccines shedding influenza virus experienced a rise in hemagglutinin-inhibition antibody 15 to 30 days after vaccination. Vaccinees showed low but significant lymphocyte transformation to A/USSR (H1N1) by day 8 after vaccination, which decreased to prevaccination levels at 30 to 34 days. The lymphocyte transformation response occurred before serum antibody rises were detected by hemagglutinin-inhibition assay. No change in lymphocyte responsiveness was observed after vaccination as measured by phytohemagglutinin stimulation. Lymphocytes responded to in vitro incubation with inactivated influenza (H1N1) virus by producing interferon. The interferon produced was of type I and was observed in vaccinees and nonvaccinees both before and after vaccination.

Full text

PDF
862

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beare A. S., Maassab H. F., Tyrrell D. A., Slepuskin A. N., Hall T. S. A comparative study of attenuated influenza viruses. Bull World Health Organ. 1971;44(5):593–598. [PMC free article] [PubMed] [Google Scholar]
  2. Chow T. C., Beutner K. R., Ogra P. L. Cell-mediated immune responses to the hemagglutinin and neuraminidase antigens of influenza A virus after immunization in humans. Infect Immun. 1979 Jul;25(1):103–109. doi: 10.1128/iai.25.1.103-109.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davenport F. M., Hennessy A. V., Maassab H. F., Minuse E., Clark L. C., Abrams G. D., Mitchell J. R. Pilot studies on recombinant cold-adapted live type A and B influenza virus vaccines. J Infect Dis. 1977 Jul;136(1):17–25. doi: 10.1093/infdis/136.1.17. [DOI] [PubMed] [Google Scholar]
  4. Dolin R., Murphy B. R., Caplan E. A. Lymphocyte blastogenic responses to influenza virus antigens after influenza infection and vaccination in humans. Infect Immun. 1978 Mar;19(3):867–874. doi: 10.1128/iai.19.3.867-874.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dolin R., Richman D. D., Murphy B. R., Fauci A. S. Cell-mediated immune responses in humans after induced infection with influenza A virus. J Infect Dis. 1977 May;135(5):714–719. doi: 10.1093/infdis/135.5.714. [DOI] [PubMed] [Google Scholar]
  6. Hall C. B., Douglas R. G., Jr, Simons R. L., Geiman J. M. Interferon production in children with respiratory syncytial, influenza, and parainfluenza virus infections. J Pediatr. 1978 Jul;93(1):28–32. doi: 10.1016/s0022-3476(78)80594-0. [DOI] [PubMed] [Google Scholar]
  7. Jao R. L., Wheelock E. F., Jackson G. G. Production of interferon in volunteers infected with Asian influenza. J Infect Dis. 1970 Apr;121(4):419–426. doi: 10.1093/infdis/121.4.419. [DOI] [PubMed] [Google Scholar]
  8. Kantzler G. B., Lauteria S. F., Cusumano C. L., Lee J. D., Ganguly R., Waldman R. H. Immunosuppression during influenza virus infection. Infect Immun. 1974 Nov;10(5):996–1002. doi: 10.1128/iai.10.5.996-1002.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kauffman C. A., Linnemann C. C., Jr, Schiff G. M., Phair J. P. Effect of viral and bacterial pneumonias on cell-mediated immunity in humans. Infect Immun. 1976 Jan;13(1):78–83. doi: 10.1128/iai.13.1.78-83.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kauffman C. A., Linnemann C. C., Jr, Tan J. S., Schiff G. M., Phair J. P. Cell-mediated immunity in humans during viral infection: dermal hypersensitivity and in vitro lymphocyte proliferation during mild viral respiratory infections. Infect Immun. 1974 Oct;10(4):757–761. doi: 10.1128/iai.10.4.757-761.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kim H. W., Arrobio J. O., Brandt C. D., Wright P., Hodes D., Chanock R. M., Parrott R. H. Safety and antigenicity of temperature sensitive (TS) mutant respiratory syncytial virus (RSV) in infants and children. Pediatrics. 1973 Jul;52(1):56–63. [PubMed] [Google Scholar]
  12. Kitayama T., Togo Y., Hornick R. B., Friedwald W. T. Low-Temperature-Adapted Influenza A2/AA/6/60 Virus Vaccine in Man. Infect Immun. 1973 Jan;7(1):119–122. doi: 10.1128/iai.7.1.119-122.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maassab H. F. Adaptation and growth characteristics of influenza virus at 25 degrees c. Nature. 1967 Feb 11;213(5076):612–614. doi: 10.1038/213612a0. [DOI] [PubMed] [Google Scholar]
  14. Maassab H. F. Biologic and immunologic characteristics of cold-adapted influenza virus. J Immunol. 1969 Mar;102(3):728–732. [PubMed] [Google Scholar]
  15. McIntosh K. Interferon in nasal secretions from infants with viral respiratory tract infections. J Pediatr. 1978 Jul;93(1):33–36. doi: 10.1016/s0022-3476(78)80595-2. [DOI] [PubMed] [Google Scholar]
  16. Murphy B. R., Baron S., Chalhub E. G., Uhlendorf C. P., Chanock R. M. Temperature-sensitive mutants of influenza virus. IV. Induction of interferon in the nasopharynx by wild-type and a temperature-sensitive recombinant virus. J Infect Dis. 1973 Oct;128(4):488–493. doi: 10.1093/infdis/128.4.488. [DOI] [PubMed] [Google Scholar]
  17. Notkins A. L., Mergenhagen S. E., Howard R. J. Effect of virus infections on the function of the immune system. Annu Rev Microbiol. 1970;24:525–538. doi: 10.1146/annurev.mi.24.100170.002521. [DOI] [PubMed] [Google Scholar]
  18. Rasmussen L. E., Jordan G. W., Stevens D. A., Merigan T. C. Lymphocyte interferon production and transformation after Herpes simplex infections in humans. J Immunol. 1974 Feb;112(2):728–736. [PubMed] [Google Scholar]
  19. Reed W. P., Olds J. W., Kisch A. L. Decreased skin-test reactivity associated with influenza. J Infect Dis. 1972 Apr;125(4):398–402. doi: 10.1093/infdis/125.4.398. [DOI] [PubMed] [Google Scholar]
  20. Ruben F. L., Jackson G. G., Gotoff S. P. Humoral and cellular response in humans after immunization with influenza vaccine. Infect Immun. 1973 Apr;7(4):594–596. doi: 10.1128/iai.7.4.594-596.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wheelock E. F., Toy S. T. Participation of lymphocytes in viral infections. Adv Immunol. 1973;16:123–184. doi: 10.1016/s0065-2776(08)60297-7. [DOI] [PubMed] [Google Scholar]
  22. Wright P. F., Sell S. H., Shinozaki T., Thompson J., Karzon D. T. Safety and antigenicity of influenza A/Hong Kong/68-ts-1 (E) (H3N2). J Pediatr. 1975 Dec;87(6 Pt 2):1109–1116. doi: 10.1016/s0022-3476(75)80123-5. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES