Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Mar;27(3):897–902. doi: 10.1128/iai.27.3.897-902.1980

Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum.

J H Crosa, L L Hodges, M H Schiewe
PMCID: PMC550859  PMID: 6247282

Abstract

The transposon A sequence Tn1 containing the ampicillin resistance determinants was transposed from RP4 to a plasmid of the marine fish pathogen Vibrio anguillarum. Curing experiments in which plasmid loss was determined by analysis of the segregation of the ampicillin resistance phenotype showed the association of virulence with the specific V. anguillarum plasmid class.

Full text

PDF
897

Images in this article

899Fig. 1
on p.899
900Fig. 2
on p.900

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cisar J. O., Fryer J. L. An epizootic of vibriosis in chinook salmon. Wildl Dis. 1969 Apr;5(2):73–76. doi: 10.7589/0090-3558-5.2.73. [DOI] [PubMed] [Google Scholar]
  2. Crosa J. H., Brenner D. J., Falkow S. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol. 1973 Sep;115(3):904–911. doi: 10.1128/jb.115.3.904-911.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crosa J. H., Olarte J., Mata L. J., Luttropp L. K., Peñaranda M. E. Characterization of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae type 1 isolated from epidemics. Antimicrob Agents Chemother. 1977 Mar;11(3):553–558. doi: 10.1128/aac.11.3.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crosa J. H., Schiewe M. H., Falkow S. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. Infect Immun. 1977 Nov;18(2):509–513. doi: 10.1128/iai.18.2.509-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gunnels R. D., Hodgins H. O., Schiewe M. H. Failure of vaccines to protect salmon from vibriosis enzootic in Puget Sound, Washington. Am J Vet Res. 1976 Jun;37(6):737–740. [PubMed] [Google Scholar]
  8. Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
  9. Heffron F., Bedinger P., Champoux J. J., Falkow S. Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci U S A. 1977 Feb;74(2):702–706. doi: 10.1073/pnas.74.2.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heffron F., Sublett R., Hedges R. W., Jacob A., Falkow S. Origin of the TEM-beta-lactamase gene found on plasmids. J Bacteriol. 1975 Apr;122(1):250–256. doi: 10.1128/jb.122.1.250-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson S. R., Romig W. R. Vibrio cholerae hybrid sex factor that contains ampicillin transposon Tn1. J Bacteriol. 1979 Jan;137(1):531–536. doi: 10.1128/jb.137.1.531-536.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rubens C., Heffron F., Falkow S. Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: independence from host rec functions and orientation of insertion. J Bacteriol. 1976 Oct;128(1):425–434. doi: 10.1128/jb.128.1.425-434.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schiewe M. H., Crosa J. H., Ordal E. J. Deoxyribonucleic acid relationships among marine vibrios pathogenic to fish. Can J Microbiol. 1977 Aug;23(8):954–958. doi: 10.1139/m77-142. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES