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. 1980 Apr;28(1):11–16. doi: 10.1128/iai.28.1.11-16.1980

Enhancement of cellular protein synthesis sensitivity to diphtheria toxin by interferon.

M Aboud, T Michalski-Stern, Y Nitzan, S Salzberg
PMCID: PMC550886  PMID: 6155343

Abstract

In attempts to determine whether, by analogy to cholera and tetanus toxins, diphtheria toxin (DT) can also relieve the antiviral effect of interferon (IF), we found that it rather enhanced the inhibitory effect of IF on the replication of murine leukemia virus in chronically infected NIH/3T3 cells. This enhancement was found to be a consequence of an increased sensitivity to DT of cellular protein synthesis in IF-treated cells. IF stimulated the anti-protein synthesis activity of DT in both mouse cells that are known to be highly resistant to this toxin and in human HeLa cells that are highly sensitive to this toxin. This stimulation was dependent on IF dose. The effect of IF on DT action was strictly species specific, indicating that it was not a consequence of the mere binding of IF to the cell membrane, but rather reflected the cellular changes that followed this initial binding. IF was found to be capable of potentiating intact DT, but could not potentiate its fragments in any combination. IF did not have any effect on the in vitro nicotinamide adenine dinucleotide glycohydrolase activity of DT, suggesting that the effect of IF is not due to molecular modification of the toxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aboud M., Weiss O., Salzberg S. Rapid quantitation of interferon with chronically oncornavirus-producing cells. Infect Immun. 1976 Jun;13(6):1626–1632. doi: 10.1128/iai.13.6.1626-1632.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ankel H., Chany C., Galliot B., Chevalier M. J., Robert M. Antiviral effect of interferon covalently bound to sepharose. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2360–2363. doi: 10.1073/pnas.70.8.2360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Besancon F., Ankel H., Basu S. Specificity and reversibility of interferon ganglioside interaction. Nature. 1976 Feb 19;259(5544):576–578. doi: 10.1038/259576a0. [DOI] [PubMed] [Google Scholar]
  4. Besançon F., Ankel H. Inhibition de l'action de l'interféron par des hormones glycoprotéiques. C R Acad Sci Hebd Seances Acad Sci D. 1976 Dec 20;283(16):1807–1810. [PubMed] [Google Scholar]
  5. Besançon F., Ankel H. Membrane receptors for interferon. Tex Rep Biol Med. 1977;35:282–292. [PubMed] [Google Scholar]
  6. Chang E. H., Jay F. T., Friedman R. M. Physical, morphological, and biochemical alterations in the membrane of AKR mouse cells after interferon treatment. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1859–1863. doi: 10.1073/pnas.75.4.1859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chany C., Ankel H., Galliot B., Chevalier M. J., Gregoire A. Mode of action and biological properties of insoluble interferon. Proc Soc Exp Biol Med. 1974 Oct;147(1):293–299. doi: 10.3181/00379727-147-38329. [DOI] [PubMed] [Google Scholar]
  8. Chany C. Membrane-bound interferon specific cell receptor system: role in the establishment and amplification of the antiviral state. Biomedicine. 1976 Jun;24(3):148–157. [PubMed] [Google Scholar]
  9. Chany C., Pauloin A., Chany-Fournier F. Role of the membrane-bound receptor system in the biological activity of interferon. Tex Rep Biol Med. 1977;35:330–335. [PubMed] [Google Scholar]
  10. Collier R. J. Diphtheria toxin: mode of action and structure. Bacteriol Rev. 1975 Mar;39(1):54–85. doi: 10.1128/br.39.1.54-85.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Collier R. J., Kandel J. Structure and activity of diphtheria toxin. I. Thiol-dependent dissociation of a fraction of toxin into enzymically active and inactive fragments. J Biol Chem. 1971 Mar 10;246(5):1496–1503. [PubMed] [Google Scholar]
  12. Dahl H. Differentiation between antiviral and anticellular effects of interferon. Tex Rep Biol Med. 1977;35:381–387. [PubMed] [Google Scholar]
  13. Degré M. Cholera toxin inhibits antiviral and growth inhibitory activities of human interferon. Proc Soc Exp Biol Med. 1978 Feb;157(2):253–255. [PubMed] [Google Scholar]
  14. Friedman R. M. Antiviral activity of interferons. Bacteriol Rev. 1977 Sep;41(3):543–567. doi: 10.1128/br.41.3.543-567.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Friedman R. M. Interferon binding: the first step in establishment of antiviral activity. Science. 1967 Jun 30;156(3783):1760–1761. doi: 10.1126/science.156.3783.1760. [DOI] [PubMed] [Google Scholar]
  16. Friedman R. M., Kohn L. D. Cholera toxin inhibits interferon action. Biochem Biophys Res Commun. 1976 Jun 21;70(4):1078–1084. doi: 10.1016/0006-291x(76)91012-3. [DOI] [PubMed] [Google Scholar]
  17. Knight E., Jr, Korant B. D. A cell surface alteration in mouse L cells induced by interferon. Biochem Biophys Res Commun. 1977 Jan 24;74(2):707–713. doi: 10.1016/0006-291x(77)90360-6. [DOI] [PubMed] [Google Scholar]
  18. Kohn L. D., Friedman R. M., Holmes J. M., Lee G. Use of thyrotropin and cholera toxin to probe the mechanism by which interferon initiates its antiviral activity. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3695–3699. doi: 10.1073/pnas.73.10.3695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lebon P., Moreau M. C., Cohen L., Chany C. Different effect of ouabain on the interferon production and action. Proc Soc Exp Biol Med. 1975 May;149(1):108–112. doi: 10.3181/00379727-149-38753. [DOI] [PubMed] [Google Scholar]
  20. Lee G., Aloj S. M., Brady R. O., Kohn L. D. The structure and function of glycoprotein hormone receptors: ganglioside interactions with human chorionic gonadotropin. Biochem Biophys Res Commun. 1976 Nov 22;73(2):370–377. doi: 10.1016/0006-291x(76)90717-8. [DOI] [PubMed] [Google Scholar]
  21. Moehring T. J., Moehring J. M., Stinebring W. R. Response of interferon-treated cells to diphtheria toxin. Infect Immun. 1971 Dec;4(6):747–752. doi: 10.1128/iai.4.6.747-752.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mullin B. R., Fishman P. H., Lee G., Aloj S. M., Ledley F. D., Winand R. J., Kohn L. D., Brady R. O. Thyrotropin-ganglioside interactions and their relationship to the structure and function of thyrotropin receptors. Proc Natl Acad Sci U S A. 1976 Mar;73(3):842–846. doi: 10.1073/pnas.73.3.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pappenheimer A. M., Jr Diphtheria toxin. Annu Rev Biochem. 1977;46:69–94. doi: 10.1146/annurev.bi.46.070177.000441. [DOI] [PubMed] [Google Scholar]
  24. Paucker K., Dalton B. J., Ogburn C. A., Törmä E. Multiple active sites on human interferons. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4587–4591. doi: 10.1073/pnas.72.11.4587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pitha P. M., Vengris V. E., Reynolds F. H., Jr The role of cell membrane in the antiviral effect of interferon. J Supramol Struct. 1976;4(4):467–473. doi: 10.1002/jss.400040405. [DOI] [PubMed] [Google Scholar]
  26. Salzberg S., Bakhanashvili M., Aboud M. Effect of interferon on mouse cells chronically infected with murine leukaemia virus: kinetic studies on virus production and virus RNA synthesis. J Gen Virol. 1978 Jul;40(1):121–130. doi: 10.1099/0022-1317-40-1-121. [DOI] [PubMed] [Google Scholar]
  27. Salzberg S., Heller A., Aboud M., Gurari-Rotman D., Revel M. Effect of interferon on human cells releasing oncornaviruses: an assay for human interferon. Virology. 1979 Feb;93(1):209–214. doi: 10.1016/0042-6822(79)90288-5. [DOI] [PubMed] [Google Scholar]
  28. Stewart W. E., 2nd, Gresser I., Tovey M. G., Bandu M., Le Goff S. Identification of the cell multiplication inhibitory factors in interferon preparations as interferons. Nature. 1976 Jul 22;262(5566):300–302. doi: 10.1038/262300a0. [DOI] [PubMed] [Google Scholar]
  29. Vengris V. E., Stollar B. D., Pitha P. M. Interferon externalization by producing cell before induction of antiviral state. Virology. 1975 Jun;65(2):410–417. doi: 10.1016/0042-6822(75)90046-x. [DOI] [PubMed] [Google Scholar]

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