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. 1980 Apr;28(1):38–44. doi: 10.1128/iai.28.1.38-44.1980

Participation of three major glycoprotein antigens of herpes simplex virus type 1 early in the infectious cycle as determined by antibody-dependent cell-mediated cytotoxicity.

B Norrild, S L Shore, T L Cromeans, A J Nahmias
PMCID: PMC550890  PMID: 6247286

Abstract

Tissue culture cells infected with herpes simplex virus type 1 synthesize three major glycoprotein antigens (Ag-11, Ag-8, and Ag-6), which have been characterized by crossed immunoelectrophoresis. The three viral antigens have been identified as a mixture of gA and gB (Ag-11), gD (Ag-8), and gC (Ag-6). Recent findings have shown that antibodies directed to each of the three antigens individually are able to mediate antibody-dependent cell-mediated cytotoxicity when tissue culture cells late in the infectious cycle (18 h postinfection) are used. In this work, antibody-dependent cell-mediated cytotoxicity was applied to study the time postinfection at which the individual viral antigens first made their appearance at the cell surface. All three viral antigens (Ag-11, Ag-8, and Ag-6) could be demonstrated as newly synthesized from 3 to 4 h postinfection, and the quantities of the antigens at the surfaces of the infected cells increased with time postinfection. The use of cycloheximide and ultraviolet-inactivated virus demonstrated that input virus could be detected by antibody-dependent cell-mediated cytotoxicity during the first 2 h postinfection, but the cytotoxicity caused by input virus remained constant with time postinfection. In conclusion, these observations demonstrate the participation of individual herpes simplex virus surface antigens in antibody-dependent cell-mediated cytotoxicity attack on cells early in infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Glorioso J. C., Smith J. W. Immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens. J Immunol. 1977 Jan;118(1):114–121. [PubMed] [Google Scholar]
  2. Harboe N., Ingild A. Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand J Immunol Suppl. 1973;1:161–164. doi: 10.1111/j.1365-3083.1973.tb03798.x. [DOI] [PubMed] [Google Scholar]
  3. Harris R., Ukaejiofo E. O. Rapid preparation of lymphocytes for tissue-typing. Lancet. 1969 Aug 9;2(7615):327–327. doi: 10.1016/s0140-6736(69)90096-8. [DOI] [PubMed] [Google Scholar]
  4. Heine J. W., Spear P. G., Roizman B. Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. J Virol. 1972 Mar;9(3):431–439. doi: 10.1128/jvi.9.3.431-439.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Honess R. W., Roizman B. Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. J Virol. 1974 Jul;14(1):8–19. doi: 10.1128/jvi.14.1.8-19.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kohl S., Starr S. E., oleske J. M., Shore S. L., Ashman R. B., Nahmias A. J. Human monocyte-macrophage-mediated antibody-dependent cytotoxicity to herpes simplex virus-infected cells. J Immunol. 1977 Mar;118(3):729–735. [PubMed] [Google Scholar]
  7. Nahmias A. J., Chiang W. T., Del Buono I., Duffey A. Typing of Herpesvirus hominis strains by a direct immunofluorescent technique. Proc Soc Exp Biol Med. 1969 Oct;132(1):386–390. doi: 10.3181/00379727-132-34221. [DOI] [PubMed] [Google Scholar]
  8. Norrild B., Bjerrum O. J., Ludwig H., Vestergaard B. F. Analysis of herpes simplex virus type 1 antigens exposed on the surface of infected tissue culture cells. Virology. 1978 Jun 15;87(2):307–316. doi: 10.1016/0042-6822(78)90136-8. [DOI] [PubMed] [Google Scholar]
  9. Norrild B., Ludwig H., Rott R. Identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and B virus. J Virol. 1978 Jun;26(3):712–717. doi: 10.1128/jvi.26.3.712-717.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Norrild B., Shore S. L., Nahmias A. J. Herpes simplex virus glycoproteins: participation of individual herpes simplex virus type 1 glycoprotein antigens in immunocytolysis and their correlation with previously identified glycopolypeptides. J Virol. 1979 Dec;32(3):741–748. doi: 10.1128/jvi.32.3.741-748.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oleske J. M., Ashman R. B., Kohl S., Shore S. L., Starr S. E., Wood P., Nahmias A. J. Human polymorphonuclear leucocytes as mediators of antibody-dependent cellular cytotoxicity to herpes simplex virus-infected cells. Clin Exp Immunol. 1977 Mar;27(3):446–453. [PMC free article] [PubMed] [Google Scholar]
  12. Pauli G., Ludwig H. Immunoprecipitation of herpes simplex virus type 1 antigens with different antisera and human cerebrospinal fluids. Arch Virol. 1977;53(1-2):139–155. doi: 10.1007/BF01314855. [DOI] [PubMed] [Google Scholar]
  13. Romano T. J., Shore S. L. Lysis of virus-infected target cells by antibody-dependent cellular cytotoxicity. I. General requirements of the reaction and temporal relationship between lethal hits and cytolysis. Cell Immunol. 1977 Apr;30(1):66–81. doi: 10.1016/0008-8749(77)90048-x. [DOI] [PubMed] [Google Scholar]
  14. Shore S. L., Black C. M., Melewicz F. M., Wood P. A., Nahmias A. J. Antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. J Immunol. 1976 Jan;116(1):194–201. [PubMed] [Google Scholar]
  15. Shore S. L., Cromeans T. L., Norrild B. Early damage of herpes-infected cells by antibody-dependent cellular cytotoxicity: relative roles of virus-specified cell-surface antigens and input virus. J Immunol. 1979 Nov;123(5):2239–2244. [PubMed] [Google Scholar]
  16. Shore S. L., Cromeans T. L., Romano T. J. Immune destruction of virus-infected cells early in the infectious cycle. Nature. 1976 Aug 19;262(5570):695–696. doi: 10.1038/262695a0. [DOI] [PubMed] [Google Scholar]
  17. Spear P. G. Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells. J Virol. 1976 Mar;17(3):991–1008. doi: 10.1128/jvi.17.3.991-1008.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Subramanian T., Rawls W. E. Comparison of antibody-dependent cellular cytotoxicity and complement-dependent antibody lysis of herpes simplex virus-infected cells as methods of detecting antiviral antibodies in human sera. J Clin Microbiol. 1977 Jun;5(6):551–558. doi: 10.1128/jcm.5.6.551-558.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vestergaard B. F., Bjerrum O. J., Norrild B., Grauballe P. C. Crossed immunoelectrophoretic studies of the solubility immunogenicity of herpes simplex virus antigens. J Virol. 1977 Oct;24(1):82–90. doi: 10.1128/jvi.24.1.82-90.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vestergaard B. F., Norrild B. Crossed immunoelectrophoresis of a herpes simplex virus type 1-specific antigen: immunological and biochemical characterization. J Infect Dis. 1978 Nov;138(5):639–643. doi: 10.1093/infdis/138.5.639. [DOI] [PubMed] [Google Scholar]

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