Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1980 Apr;28(1):45–48. doi: 10.1128/iai.28.1.45-48.1980

Hepatitis A virus in the liver and intestine of marmosets after oral inoculation.

L R Mathiesen, A M Møller, R H Purcell, W T London, S M Feinstone
PMCID: PMC550891  PMID: 6247287

Abstract

A total of 12 seronegative marmosets (Saguinus mystax) were inoculated orally with hepatitis A virus (HAV) and sacrificed at 3- to 4-day intervals. Tissues from the livers, intestines, mesenteric lymph nodes, and spleens were obtained for immunofluorescence studies, and bile and intestinal contents were obtained for enzyme-linked immunosorbent assay studies. Two marmosets sacrificed on days 34 and 41 after inoculation developed antibody to HAV and demonstrated HAV in their livers but not in any part of their intestinal tissues. None of the remaining marmosets sacrificed from days -3 to 31 survived long enough to develop antibody to HAV, but an additional two marmosets, which were sacrificed on days 21 and 31, demonstrated HAV in their livers and also in bile but not in the intestinal tissues or their contents. The mesenteric lymph nodes and spleens were negative for HAV by immunofluorescence in all the marmosets. No evidence of HAV replication was demonstrated in any part of the intestine at any time during the incubation period or during acute illness in the marmosets inoculated orally with HAV. The shedding of HAV in stools in the late incubation period can be explained by excretion of HAV from the livers with the bile.

Full text

PDF
45

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BODIAN D. Poliovirus in chimpanzee tissues after virus feeding. Am J Hyg. 1956 Sep;64(2):181–197. doi: 10.1093/oxfordjournals.aje.a119833. [DOI] [PubMed] [Google Scholar]
  2. Bradley D. W., Gravelle C. R., Cook E. H., Fields R. M., Maynard J. E. Cyclic excretion of hepatitis A virus in experimentally infected chimpanzees: biophysical characterization of the associated HAV particles. J Med Virol. 1977;1(2):133–138. doi: 10.1002/jmv.1890010206. [DOI] [PubMed] [Google Scholar]
  3. Dienstag J. L., Feinstone S. M., Kapikian A. Z., Purcell R. H. Faecal shedding of hepatitis-A antigen. Lancet. 1975 Apr 5;1(7910):765–767. doi: 10.1016/s0140-6736(75)92434-4. [DOI] [PubMed] [Google Scholar]
  4. Dienstag J. L., Feinstone S. M., Purcell R. H., Hoofnagle J. H., Barker L. F., London W. T., Popper H., Peterson J. M., Kapikian A. Z. Experimental infection of chimpanzees with hepatitis A virus. J Infect Dis. 1975 Nov;132(5):532–545. doi: 10.1093/infdis/132.5.532. [DOI] [PubMed] [Google Scholar]
  5. Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
  6. Mathiesen L. R., Drucker J., Lorenz D., Wagner J. A., Gerety R. J., Purcell R. H. Localization of hepatitis A antigen in marmoset organs during acute infection with hepatitis A virus. J Infect Dis. 1978 Sep;138(3):369–377. doi: 10.1093/infdis/138.3.369. [DOI] [PubMed] [Google Scholar]
  7. Mathiesen L. R., Feinstone S. M., Purcell R. H., Wagner J. A. Detection of hepatitis A antigen by immunofluorescence. Infect Immun. 1977 Nov;18(2):524–530. doi: 10.1128/iai.18.2.524-530.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mathiesen L. R., Feinstone S. M., Wong D. C., Skinhoej P., Purcell R. H. Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay. J Clin Microbiol. 1978 Feb;7(2):184–193. doi: 10.1128/jcm.7.2.184-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SABIN A. B. Pathogenesis of poliomyelitis; reappraisal in the light of new data. Science. 1956 Jun 29;123(3209):1151–1157. doi: 10.1126/science.123.3209.1151. [DOI] [PubMed] [Google Scholar]
  10. WARD R., KRUGMAN S., GILES J. P., JACOBS A. M., BODANSKY O. Infectious hepatitis; studies of its natural history and prevention. N Engl J Med. 1958 Feb 27;258(9):407–416. doi: 10.1056/NEJM195802272580901. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES