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. 1980 Apr;28(1):220–224. doi: 10.1128/iai.28.1.220-224.1980

Regulation and function of ammonia-assimilating enzymes in Streptococcus mutans.

E J St Martin, C L Wittenberger
PMCID: PMC550915  PMID: 6103877

Abstract

The ability of Streptococcus mutans to synthesize amino acids was examined. A total of 8 of 12 laboratory strains grew anaerobically on solid-defined medium that contained no amino acids. Several isolates, therefore, assimilated ammonia for the biosynthesis of amino acids. These strains included representatives of five serotypes. One strain, DR0001, was also grown in liquid-defined medium. The enzymes of two pathways by which ammonia can be fixed were detected in this strain DR0001 could use either a reduced nicotinamide adenine dinucleotide phosphate-coupled glutamate dehydrogenase or the combined action of adenosine 5'-triphosphate-driven glutamine synthetase with a reduced nicotinamide adenine dinucleotide-coupled glutamate synthase to assimilate ammonia for the biosynthesis of amino acids. Evidence that both pathways were functional was provided by an analysis of the influence of the nitrogen source on enzyme levels and by the isolation and characterization of glutamate dehydrogenase-negative mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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