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. 1980 May;28(2):303–309. doi: 10.1128/iai.28.2.303-309.1980

Oral toxicities of Clostridium botulinum type C and D toxins of different molecular sizes.

I Ohishi, G Sakaguchi
PMCID: PMC550933  PMID: 7399665

Abstract

Clostridium botulinum type C progenitor toxins of different molecule sizes, C-L (16S) and C-M (12S), were purified from cultures of strains 573, Stockholm, and CB-19. C-L toxin showed some hemaggglutinin activity, whereas C-M toxin did not. Neither C-L nor C-M toxin was activated upon trypsinization. Molecular dissociation of purified type C-L and C-M toxins into toxic and nontoxic components was demonstrated by sucrose density gradient ultracentrifugation and diethylaminoethyl-Sephadex chromatography at pH 8.0. The molecular construction of type C progenitor toxin appears to be analogous to that reported for botulinum toxins of other types. C-L and D-L toxins showed higher oral toxicities to mice than did C-M or D-M toxin. Such higher oral toxicities were ascribed to the higher stabilities of these toxins in gastric and intestinal juices.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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