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. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980

Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component.

M W Russell, L A Bergmeier, E D Zanders, T Lehner
PMCID: PMC550961  PMID: 6995311

Abstract

A surface protein antigen of Streptococcus mutans having two sets of antigenic determinants (antigens I and II) was purified by column chromatography from culture supernatants of S. mutans serotype c. The protease-resistant component, antigen II, was purified from pronase-digested antigen I/II. The antigens were analyzed chemically and immunologically, and their physicochemical properties were investigated. Antigen I/II consisted of more than 80% protein, and its peptide chain molecular weight was estimated to be 185,000. Antigen II consisted of approximately 60% protein, with a peptide chain molecular weight of 48,000. Antisera to antigens I/II and II were raised in rabbits and used to investigate the presence of the antigens in cells of other streptococci. This indicated that not only serotype c but also serotypes e and f possessed antigen I and II determinants, whereas serotypes a, d, and g possessed a determinant related to antigen I but not one related to antigen II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J. 1965 Sep;96(3):595–606. doi: 10.1042/bj0960595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowden G. H., Hardie J. M., Fillery E. D. Antigens from Actinomyces species and their value in identification. J Dent Res. 1976 Jan;55:A192–A204. doi: 10.1177/002203457605500112011. [DOI] [PubMed] [Google Scholar]
  3. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  4. Bratthall D., Pettersson B. M. Common and unique antigens of Streptococcus mutants. J Dent Res. 1976 Jan;55:A60–A64. doi: 10.1177/002203457605500123011. [DOI] [PubMed] [Google Scholar]
  5. Chorpenning F. W., Cooper H. R., Rosen S. Cross-reactions of Streptococcus mutans due to cell wall teichoic acid. Infect Immun. 1975 Sep;12(3):586–591. doi: 10.1128/iai.12.3.586-591.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hardie J. M., Bowden G. H. Cell wall and serological studies on Streptococcus mutans. Caries Res. 1974;8(4):301–316. doi: 10.1159/000260120. [DOI] [PubMed] [Google Scholar]
  7. Hardie J. M., Bowden G. H. Some serological cross-reactions between Streptococcus mutans, S sanguis, and other dental plaque streptococci. J Dent Res. 1976 Apr;55(Spec No):C50–C58. doi: 10.1177/002203457605500325011. [DOI] [PubMed] [Google Scholar]
  8. Hladny J., Schleifer K. H., Kandler O. Die Aminosäuresequenz der theoninhaltigen Murein einiger Streptokokken. Arch Mikrobiol. 1972;85(1):23–38. [PubMed] [Google Scholar]
  9. Joseph R., Shockman G. D. Synthesis and excretion of glycerol teichoic acid during growth of two streptococcal species. Infect Immun. 1975 Aug;12(2):333–338. doi: 10.1128/iai.12.2.333-338.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knox K. W., Wicken A. J. Immunological properties of teichoic acids. Bacteriol Rev. 1973 Jun;37(2):215–257. doi: 10.1128/br.37.2.215-257.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lehner T., Challacombe S. J., Caldwell J. Immunologic basis for vaccination against dental caries in rhesus monkeys. J Dent Res. 1976 Apr;55(Spec No):C166–C180. doi: 10.1177/002203457605500311011. [DOI] [PubMed] [Google Scholar]
  14. Markham J. L., Knox K. W., Wicken A. J., Hewett M. J. Formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. Infect Immun. 1975 Aug;12(2):378–386. doi: 10.1128/iai.12.2.378-386.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Perch B., Kjems E., Ravn T. Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):357–370. doi: 10.1111/j.1699-0463.1974.tb02338.x. [DOI] [PubMed] [Google Scholar]
  16. Russell M. W., Lehner T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch Oral Biol. 1978;23(1):7–15. doi: 10.1016/0003-9969(78)90047-x. [DOI] [PubMed] [Google Scholar]
  17. Russell M. W. Purification and properties of a protein surface antigen of Streptococcus mutants. Microbios. 1979;25(99):7–18. [PubMed] [Google Scholar]
  18. Russell R. R. Wall-associated protein antigens of Streptococcus mutans. J Gen Microbiol. 1979 Sep;114(1):109–115. doi: 10.1099/00221287-114-1-109. [DOI] [PubMed] [Google Scholar]
  19. Schmidt-Ullrich R., Thompson W. S., Wallach D. F. Antigenic distinctions of glycoproteins in plasma and mitochondrial membranes of lymphoid cells neoplastically transformed by simian virus 40. Proc Natl Acad Sci U S A. 1977 Feb;74(2):643–647. doi: 10.1073/pnas.74.2.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Showe M. K., Isobe E., Onorato L. Bacteriophage T4 prehead proteinase. II. Its cleavage from the product of gene 21 and regulation in phage-infected cells. J Mol Biol. 1976 Oct 15;107(1):55–69. doi: 10.1016/s0022-2836(76)80017-4. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  22. Tsuji A., Kinoshita T., Hoshino M. Analytical chemical studies on amino sugars. II. Determination of hexosamines using 3-methyl-2-benzothiazolone hydrazone hydrochloride. Chem Pharm Bull (Tokyo) 1969 Jul;17(7):1505–1510. doi: 10.1248/cpb.17.1505. [DOI] [PubMed] [Google Scholar]

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