Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 May;28(2):502–507. doi: 10.1128/iai.28.2.502-507.1980

Alterations in the Distribution and Proliferative Responses of Rhesus Monkey Peripheral Blood and Spleen Cells During Malaria (Plasmodium knowlesi) Infection

Diane W Taylor 1,†, Suzanne Richmond Crum 1, Kenton J Kramer 1, Wasim A Siddiqui 1
PMCID: PMC550963  PMID: 6772559

Abstract

Rhesus monkeys are used frequently as animal models in malaria research, but few studies have evaluated lymphocyte functions in these animals after experimental infections with the primate malarial parasite Plasmodium knowlesi. In this study, the distribution and mitogen responses of mononuclear cells in the peripheral blood and spleens of 16 P. knowlesi-infected rhesus monkeys were followed. All animals included in the study developed acute infections and were bled out with parasitemias of more than 50%. With progression of the infection, alterations in the peripheral blood mononuclear cells were observed, including decreases in the percentage of T cells (measured by E rosette formation) and the total numbers of E and EAC rosette-forming cells per cubic millimeter. In addition, peripheral blood mononuclear cells displayed reduced responses to mitogen stimulation with phytohemagglutinin, concanavalin A, and pokeweed mitogen. Peripheral blood mononuclear cells from infected animals showed similar reductions in mitogen responses when cultured in media containing 15% autologous pre- or postinfection plasma. The mitogen responses of spleen cells did not appear to be affected, but a significant reduction in the proportion of splenic T cells was observed. These lymphocyte changes in P. knowlesi-infected rhesus monkeys are similar to those reported for mice with acute rodent malaria and for humans with chronic Plasmodium falciparum infections.

Full text

PDF
502

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Bloch K. J. Immunoglobulins on the surface of neoplastic lymphocytes. N Engl J Med. 1972 Aug 10;287(6):272–276. doi: 10.1056/NEJM197208102870603. [DOI] [PubMed] [Google Scholar]
  2. Butcher G. A., Cohen S. Antigenic variation and protective immunity in Plasmodium knowlesi malaria. Immunology. 1972 Oct;23(4):503–521. [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S., Butcher G. A., Mitchell G. H. Mechanisms of immunity to malaria. Bull World Health Organ. 1974;50(3-4):251–257. [PMC free article] [PubMed] [Google Scholar]
  4. Coleman R. M., Rencricca N. J., Stout J. P., Brissette W. H., Smith D. M. Splenic mediated erythrocyte cytotoxicity in malaria. Immunology. 1975 Jul;29(1):49–54. [PMC free article] [PubMed] [Google Scholar]
  5. Ganguly N. K., Mohan C., Sapru R. P., Kumar M. T and B cell populations in the peripheral blood of rhesus monkeys. Int Arch Allergy Appl Immunol. 1977;53(3):290–292. doi: 10.1159/000231764. [DOI] [PubMed] [Google Scholar]
  6. Greenwood B. M., Vick R. M. Evidence for a malaria mitogen in human malaria. Nature. 1975 Oct 16;257(5527):592–594. doi: 10.1038/257592a0. [DOI] [PubMed] [Google Scholar]
  7. Jayawardena A. N., Targett G. A., Leuchars E., Carter R. L., Doenhoff M. J., Davies A. J. T-cell activation in murine malaria. Nature. 1975 Nov 13;258(5531):149–151. doi: 10.1038/258149a0. [DOI] [PubMed] [Google Scholar]
  8. Malaviya A. N., Kumar R., Bhuyan U. N., Mohapatra L. N. Rosette forming lymphocytes: a modified technique for better stability and reproducibility; distribution in lymphoid tissue of rhesus monkey. Indian J Med Res. 1974 Apr;62(4):640–647. [PubMed] [Google Scholar]
  9. Moore D. L., Heyworth B., Brown J. PHA-induced lymphocyte transformations in leucocyte cultures from malarious, malnourished and control Gambian children. Clin Exp Immunol. 1974 Aug;17(4):647–656. [PMC free article] [PubMed] [Google Scholar]
  10. Osunkoya B. O., Williams A. I., Reddy S. Spontaneous lymphocyte transformation in leucocyte cultures of children with falciparum malaria. Trop Geogr Med. 1972 Jun;24(2):157–161. [PubMed] [Google Scholar]
  11. Strickland G. T., DeSilva S., Sayles P. C. Lymphocyte changes in murine and human malaria. Tropenmed Parasitol. 1979 Mar;30(1):35–42. [PubMed] [Google Scholar]
  12. Taylor D. W., Marchette N. J., Siddiqui W. A. Lymphocyte isolation, rosette formation, and mitogen stimulation in Rhesus monkeys. Dev Comp Immunol. 1978 Jul;2(3):539–546. doi: 10.1016/s0145-305x(78)80014-7. [DOI] [PubMed] [Google Scholar]
  13. Terrell T. G., Holmberg C. A., Osburn B. I. Immunologic surface markers on nonhuman primate lymphocytes. Am J Vet Res. 1977 Apr;38(4):503–507. [PubMed] [Google Scholar]
  14. Weinbaum F. I., Weintraub J., Nkrumah F. K., Evans C. B., Tigelaar R. E., Rosenberg Y. J. Immunity to Plasmodium berghei yoelii in mice. II. Specific and nonspecific cellular and humoral responses during the course of infection. J Immunol. 1978 Aug;121(2):629–636. [PubMed] [Google Scholar]
  15. Wyler D. J., Gallin J. I. Spleen-derived mononuclear cell chemotactic factor in malaria infections: a possible mechanism for splenic macrophage accumulation. J Immunol. 1977 Feb;118(2):478–484. [PubMed] [Google Scholar]
  16. Wyler D. J., Herrod H. G., Weinbaum F. I. Response of sensitized and unsensitized human lymphocyte subpopulations to Plasmodium falciparum antigens. Infect Immun. 1979 Apr;24(1):106–110. doi: 10.1128/iai.24.1.106-110.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wyler D. J. Peripheral lymphocyte subpopulations in human falciparum malaria. Clin Exp Immunol. 1976 Mar;23(3):471–476. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES