Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 May;28(2):633–637. doi: 10.1128/iai.28.2.633-637.1980

Participation of normal human immunoglobulins M, G, and A in opsonophagocytosis and intracellular killing of Bacteroides fragilis and Bacteroides thetaiotaomicron by human polymorphonuclear leukocytes.

A B Bjornson, H S Bjornson, B P Kitko
PMCID: PMC550982  PMID: 7399680

Abstract

Restoration of the ability of hypogammaglobulinemic serum to support opsonophagocytosis and intracellular killing of Bacteroides fragilis and Bacteroides thetaiotaomicron by human polymorphonuclear leukocytes was achieved by supplementation with normal human immunoglobulin M, but not with normal human immunoglobulin G. Polymorphonuclear leukocyte bactericidal activity in the presence of immunoglobulin A-deficient human serum was equivalent to that observed in the presence of normal human serum.

Full text

PDF
633

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bjornson A. B., Altemeier W. A., Bjornson H. S. Comparison of the in vitro bactericidal activity of human serum and leukocytes against bacteroides fragilis and Fusobacterium mortiferum in aerobic and anaerobic environments. Infect Immun. 1976 Sep;14(3):843–847. doi: 10.1128/iai.14.3.843-847.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bjornson A. B., Bjornson H. S. Participation of immunoglobulin and the alternative complement pathway in opsonization of Bacteroides fragilis and Bacteroides thetaiotaomicron. Rev Infect Dis. 1979 Mar-Apr;1(2):347–356. doi: 10.1093/clinids/1.2.347. [DOI] [PubMed] [Google Scholar]
  3. Bjornson A. B., Bjornson H. S. Participation of immunoglobulin and the alternative complement pathway in opsonization of Bacteroides fragilis and Bacteroides thetaiotaomicron. J Infect Dis. 1978 Sep;138(3):351–358. doi: 10.1093/infdis/138.3.351. [DOI] [PubMed] [Google Scholar]
  4. Bjornson A. B., Michael J. G. Factors in human serum promoting phagocytosis of Pseudomonas aeruginosa. I. Interaction of opsonins with the bacterium. J Infect Dis. 1974 Nov;130 (Suppl)(0):S119–S126. doi: 10.1093/infdis/130.supplement.s119. [DOI] [PubMed] [Google Scholar]
  5. Blazevic D. J. Antibiotic susceptibility of the subspecies of Bacteroides fragilis. Antimicrob Agents Chemother. 1976 Mar;9(3):481–484. doi: 10.1128/aac.9.3.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chow A. W., Guze L. B. Bacteroidaceae bacteremia: clinical experience with 112 patients. Medicine (Baltimore) 1974 Mar;53(2):93–126. [PubMed] [Google Scholar]
  7. Ehrnst A. Separate pathways of C activation by measles virus cytotoxic antibodies: subclass analysis and capacity of F(ab) molecules to activate C via the alternative pathway. J Immunol. 1978 Sep;121(3):1206–1212. [PubMed] [Google Scholar]
  8. FLODIN P., KILLANDER J. Fractionation of human-serum proteins by gel filtration. Biochim Biophys Acta. 1962 Oct 8;63:402–410. doi: 10.1016/0006-3002(62)90104-x. [DOI] [PubMed] [Google Scholar]
  9. Flemmings B., Diggs C. Antibody-dependent cytotoxicity against Trypanosoma rhodesiense mediated through an alternative complement pathway. Infect Immun. 1978 Mar;19(3):928–933. doi: 10.1128/iai.19.3.928-933.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hill A. W., Shears A. L., Hibbitt K. G. The requirement of specific antibody for the killing of E. coli by the alternate complement pathway in bovine serum. Immunology. 1978 Jan;34(1):131–136. [PMC free article] [PubMed] [Google Scholar]
  11. Ingwer I., Petersen B. H., Brooks G. Serum bactericidal action and activation of the classic and alternate complement pathways by Neisseria gonorrhoeae. J Lab Clin Med. 1978 Aug;92(2):211–220. [PubMed] [Google Scholar]
  12. Jones R. N., Fuchs P. C. Identification and antimicrobial susceptibility of 250 Bacteriodes fragilis subspecies tested by broth microdilution methods. Antimicrob Agents Chemother. 1976 Apr;9(4):719–721. doi: 10.1128/aac.9.4.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Joseph B. S., Cooper N. R., Oldstone M. B. Immunologic injury of cultured cells infected with measles virus. I. role of IfG antibody and the alternative complement pathway. J Exp Med. 1975 Apr 1;141(4):761–774. [PMC free article] [PubMed] [Google Scholar]
  14. Klebanoff S. J., Clark R. A. Iodination by human polymorphonuclear leukocytes: a re-evaluation. J Lab Clin Med. 1977 Mar;89(3):675–686. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  17. Martin W. J. Isolation and indentification of anaerobic bacteria in the clinical laboratory. A 2-year experience. Mayo Clin Proc. 1974 May;49(5):300–308. [PubMed] [Google Scholar]
  18. Patrick Sissons J. G., Schreiber R. D., Perrin L. H., Cooper N. R., Müller-Eberhard H. J., Oldstone M. B. Lysis of measles virus-infected cells by the purified cytolytic alternative complement pathway and antibody. J Exp Med. 1979 Sep 19;150(3):445–454. doi: 10.1084/jem.150.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Polhill R. B., Jr, Newman S. L., Pruitt K. M., Johnston R. B., Jr Kinetic assessment of alternative complement pathway activity in a hemolytic system. II. Influence of antibody on alternative pathway activation. J Immunol. 1978 Jul;121(1):371–376. [PubMed] [Google Scholar]
  20. Polk B. F., Kasper D. L. Bacteroides fragilis subspecies in clinical isolates. Ann Intern Med. 1977 May;86(5):569–571. doi: 10.7326/0003-4819-86-5-569. [DOI] [PubMed] [Google Scholar]
  21. Ruley E. J., Forristal J., Davis N. C., Andres C., West C. D. Hypocomplementemia of membranoproliferative nephritis. Dependence of the nephritic factor reaction on properdin factor B. J Clin Invest. 1973 Apr;52(4):896–904. doi: 10.1172/JCI107254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schiller N. L., Friedman G. L., Roberts R. B. The role of natural IgG and complement in the phagocytosis of type 4 Neisseria gonorrhoeae by human polymorphonuclear leukocytes. J Infect Dis. 1979 Nov;140(5):698–707. doi: 10.1093/infdis/140.5.698. [DOI] [PubMed] [Google Scholar]
  23. Winkelstein J. A., Shin H. S., Wood W. B., Jr Heat labile opsonins to Pneumococcus. 3. The participation of immunoglobulin and of the alternate pathway of C3 activation. J Immunol. 1972 Jun;108(6):1681–1689. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES