Abstract
The ability of a mouse mammary tumor cell line to abrogate antibody neutralization of vesicular stomatitis virus was shown to be due to the presence of mycoplasma. The mycoplasma was isolated from the cell line and typed as Mycoplasma orale. Colonies of this mycoplasma were used to deliberately infect cell cultures which then gained the capacity to reactivate antibody-neutralized virus. The extent of the reactivation depended on the source of neutralizing antiserum. Other species of mycoplasma were tested and were found to reactivate neutralized virus, indicating that this may be a general phenomenon of mycoplasma contamination.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balduzzi P. C., Murphy H. Plaque assay of avian sarcoma viruses using casein. J Virol. 1975 Sep;16(3):707–711. doi: 10.1128/jvi.16.3.707-711.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deutsch V., Berkaloff A. Analyse d'un mutant thermolabile du virus de la stomatite vésiculaire (VSV. Ann Inst Pasteur (Paris) 1971 Jul;121(1):101–106. [PubMed] [Google Scholar]
- Eisen H., Tallan I. Tetrahymena pyriformis recovers from antibody immobilisation by producing univalent antibody fragments. Nature. 1977 Dec 8;270(5637):514–515. doi: 10.1038/270514a0. [DOI] [PubMed] [Google Scholar]
- FOGH J., FOGH H. A METHOD FOR DIRECT DEMONSTRATION OF PLEUROPNEUMONIA-LIKE ORGANISMS IN CULTURED CELLS. Proc Soc Exp Biol Med. 1964 Dec;117:899–901. doi: 10.3181/00379727-117-29731. [DOI] [PubMed] [Google Scholar]
- Flamand A. Etude génétique du virus de la stomatite vésiculaire: classement de mutants thermosensibles spontanés en groupes de complémentation. J Gen Virol. 1970 Sep;8(3):187–195. doi: 10.1099/0022-1317-8-3-187. [DOI] [PubMed] [Google Scholar]
- Goldberg A. R. Increased protease levels in transformed cells: a casein overlay assay for the detection of plasminogen activator production. Cell. 1974 Jun;2(2):95–102. doi: 10.1016/0092-8674(74)90097-x. [DOI] [PubMed] [Google Scholar]
- House W., Waddell A. Detection of mycoplasma in cell cultures. J Pathol Bacteriol. 1967 Jan;93(1):125–132. doi: 10.1002/path.1700930112. [DOI] [PubMed] [Google Scholar]
- KJELLEN L. E., SCHLESINGER R. W. Influence of host cell on residual infectivity of neutralized vesicular stomatitis virus. Virology. 1959 Feb;7(2):236–239. doi: 10.1016/0042-6822(59)90189-8. [DOI] [PubMed] [Google Scholar]
- Nardone R. M., Todd J., Gonzalez P., Gaffney E. V. Nucleoside incorporation into strain L cells: inhibition by pleuropneumonia-like organisms. Science. 1965 Sep 3;149(3688):1100–1101. doi: 10.1126/science.149.3688.1100. [DOI] [PubMed] [Google Scholar]
- Ringold G., Lasfargues E. Y., Bishop J. M., Varmus H. E. Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization. Virology. 1975 May;65(1):135–147. doi: 10.1016/0042-6822(75)90014-8. [DOI] [PubMed] [Google Scholar]
- Weiss R. A., Boettiger D., Murphy H. M. Pseudotypes of avian sarcoma viruses with the envelope properties of vesicular stomatitis virus. Virology. 1977 Feb;76(2):808–825. doi: 10.1016/0042-6822(77)90261-6. [DOI] [PubMed] [Google Scholar]
- Závada J., Dickson C., Weiss R. Pseudotypes of vesicular stomatitis virus with envelope antigens provided by murine mammary tumor virus. Virology. 1977 Oct 1;82(1):221–231. doi: 10.1016/0042-6822(77)90045-9. [DOI] [PubMed] [Google Scholar]
- Závada J. VSV pseudotype particles with the coat of avian myeloblastosis virus. Nat New Biol. 1972 Nov 22;240(99):122–124. doi: 10.1038/newbio240122a0. [DOI] [PubMed] [Google Scholar]