Abstract
We have previously described an in vitro assay for examining the mucosal adherence of a rabbit diarrheagenic Escherichia coli, RDEC-1. That assay defined the in vitro characteristics of RDEC-1 adherence to brush borders isolated from rabbit ileal epithelial cells. The present study was conducted to examine the species specificity of both in vitro RDEC-1 adherence and in vivo infectivity of RDEC-1 and to compare these specificities. Species specificity in vitro adherence was examined by using brush borders prepared from intestinal epithelial cells of rats, guinea pigs, and rabbits, as well as from a surgically resected specimen of human ileum. Strain RDEC-1 adherence to rabbit brush borders in vitro was significantly greater (P < 0.001) than its adherence to brush borders from any of the other species. Regional specificity of in vitro adherence of RDEC-1 to ileal segments of rabbit intestinal mucosa was also demonstrated. There was significantly greater adherence of RDEC-1 to rabbit ileal brush borders as compared to rabbit jejunal brush borders (P < 0.05). In vivo infectivity was assessed by inoculating RDEC-1 into rats, guinea pigs, and rabbits. RDEC-1 elicited diarrhea in all inoculated rabbits with the mean onset of illness occurring 5 days after inoculation. In contrast, none of the RDEC-1-inoculated rats or guinea pigs developed diarrhea. Furthermore, colonization studies in these animals revealed that RDEC-1 heavily colonized the ileum and cecum (109 RDEC-1 colony-forming units/g of tissue) of rabbits; however, only minimal colonization was observed in guinea pigs and rats. In conclusion, the correlation between in vitro adherence and in vivo infectivity that we have observed suggests that the presence of receptors, specific for bacteria, on the surface of the host intestinal mucosa determines species susceptibility to enteric colonization and infectivity by certain strains of enteropathogenic E. coli.
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Selected References
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- Alpers D. H., Seetharam B. Pathophysiology of diseases involving intestinal brush-border proteins. N Engl J Med. 1977 May 5;296(18):1047–1050. doi: 10.1056/NEJM197705052961808. [DOI] [PubMed] [Google Scholar]
- Bar-Shavit Z., Ofek I., Goldman R., Mirelman D., Sharon N. Mannose residues on phagocytes as receptors for the attachment of Escherichia coli and Salmonella typhi. Biochem Biophys Res Commun. 1977 Sep 9;78(1):455–460. doi: 10.1016/0006-291x(77)91276-1. [DOI] [PubMed] [Google Scholar]
- Bartelt M. A., Duncan J. L. Adherence of group A streptococci to human epithelial cells. Infect Immun. 1978 Apr;20(1):200–208. doi: 10.1128/iai.20.1.200-208.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bertschinger H. U., Moon H. W., Whipp S. C. Association of Escherichia coli with the small intestinal epithelium. II. Variations in association index and the relationship between association index and enterosorption in pigs. Infect Immun. 1972 Apr;5(4):606–611. doi: 10.1128/iai.5.4.606-611.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burrows M. R., Sellwood R., Gibbons R. A. Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. J Gen Microbiol. 1976 Oct;96(2):269–275. doi: 10.1099/00221287-96-2-269. [DOI] [PubMed] [Google Scholar]
- Cantey J. R., Blake R. K. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism. J Infect Dis. 1977 Mar;135(3):454–462. doi: 10.1093/infdis/135.3.454. [DOI] [PubMed] [Google Scholar]
- Cheney C. P., Boedeker E. C., Formal S. B. Quantitation of the adherence of an enteropathogenic Escherichia coli to isolated rabbit intestinal brush borders. Infect Immun. 1979 Nov;26(2):736–743. doi: 10.1128/iai.26.2.736-743.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deneke C. F., Thorne G. M., Gorbach S. L. Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans. Infect Immun. 1979 Oct;26(1):362–368. doi: 10.1128/iai.26.1.362-368.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donaldson R. M., Jr, Mackenzie I. L., Trier J. S. Intrinsic factor-mediated attachment of vitamin B12 to brush borders and microvillous membranes of hamster intestine. J Clin Invest. 1967 Jul;46(7):1215–1228. doi: 10.1172/JCI105615. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edén C. S., Hansson H. A. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun. 1978 Jul;21(1):229–237. doi: 10.1128/iai.21.1.229-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eichholz A. Structural and functional organization of the brush border of intestinal epithelial cells. 3. Enzymic activities and chemical composition of various fractions of tris-disrupted brush borders. Biochim Biophys Acta. 1967 Jul 3;135(3):475–482. doi: 10.1016/0005-2736(67)90037-5. [DOI] [PubMed] [Google Scholar]
- Evans D. G., Evans D. J., Jr, Tjoa W. S., DuPont H. L. Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea. Infect Immun. 1978 Feb;19(2):727–736. doi: 10.1128/iai.19.2.727-736.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forstner G. G., Tanaka K., Isselbacher K. J. Lipid composition of the isolated rat intestinal microvillus membrane. Biochem J. 1968 Aug;109(1):51–59. doi: 10.1042/bj1090051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forstner G. G., Wherrett J. R. Plasma membrane and mucosal glycosphingolipids in the rat intestine. Biochim Biophys Acta. 1973 Jun 21;306(3):446–459. doi: 10.1016/0005-2760(73)90183-5. [DOI] [PubMed] [Google Scholar]
- Fujita M., Kawai K., Asano S., Nakao M. Protein components of two different regions of an intestinal epithelial cell membrane. Regional singularities. Biochim Biophys Acta. 1973 Apr 25;307(1):141–151. doi: 10.1016/0005-2736(73)90032-1. [DOI] [PubMed] [Google Scholar]
- Gibbons R. A., Jones G. W., Sellwood R. An attempt to identify the intestinal receptor for the K88 adhesin by means of a haemagglutination inhibition test using glycoproteins and fractions from sow colostrum. J Gen Microbiol. 1975 Feb;86(2):228–240. doi: 10.1099/00221287-86-2-228. [DOI] [PubMed] [Google Scholar]
- Gould K., Ramirez-Ronda C. H., Holmes R. K., Sanford J. P. Adherence of bacteria to heart valves in vitro. J Clin Invest. 1975 Dec;56(6):1364–1370. doi: 10.1172/JCI108216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grady G. F., Keusch G. T. Pathogenesis of bacterial diarrheas. I. N Engl J Med. 1971 Oct 7;285(15):831–841. doi: 10.1056/NEJM197110072851505. [DOI] [PubMed] [Google Scholar]
- Houghton S. E., McCarthy C. F. The isolation, partial characterization, and subfractionation of human intestinal brush borders. Gut. 1973 Jul;14(7):529–534. doi: 10.1136/gut.14.7.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isaacson R. E., Fusco P. C., Brinton C. C., Moon H. W. In vitro adhesion of Escherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors. Infect Immun. 1978 Aug;21(2):392–397. doi: 10.1128/iai.21.2.392-397.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones G. W., Abrams G. D., Freter R. Adhesive properties of Vibrio cholerae: adhesion to isolated rabbit brush border membranes and hemagglutinating activity. Infect Immun. 1976 Jul;14(1):232–239. doi: 10.1128/iai.14.1.232-239.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones G. W., Freter R. Adhesive properties of Vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infect Immun. 1976 Jul;14(1):240–245. doi: 10.1128/iai.14.1.240-245.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones G. W., Rutter J. M. Role of the K88 antigen in the pathogenesis of neonatal diarrhea caused by Escherichia coli in piglets. Infect Immun. 1972 Dec;6(6):918–927. doi: 10.1128/iai.6.6.918-927.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Källenius G., Winberg J. Bacterial adherence to periurethral epithelial cells in girls prone to urinary-tract infections. Lancet. 1978 Sep 9;2(8089):540–543. doi: 10.1016/s0140-6736(78)92880-5. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Maestracci D., Preiser H., Hedges T., Schmitz J., Crane R. K. Enzymes of the human intestinal brush border membrane. Identification after gel electrophoretic separation. Biochim Biophys Acta. 1975 Mar 13;382(2):147–156. doi: 10.1016/0005-2736(75)90173-x. [DOI] [PubMed] [Google Scholar]
- McNeish A. S., Turner P., Fleming J., Evans N. Mucosal adherence of human enteropathogenic Escherichia coli. Lancet. 1975 Nov 15;2(7942):946–948. doi: 10.1016/s0140-6736(75)90360-8. [DOI] [PubMed] [Google Scholar]
- Nagy B., Moon H. W., Isaacson R. E. Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infect Immun. 1977 Apr;16(1):344–352. doi: 10.1128/iai.16.1.344-352.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ofek I., Mirelman D., Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. doi: 10.1038/265623a0. [DOI] [PubMed] [Google Scholar]
- Pearce W. A., Buchanan T. M. Attachment role of gonococcal pili. Optimum conditions and quantitation of adherence of isolated pili to human cells in vitro. J Clin Invest. 1978 Apr;61(4):931–943. doi: 10.1172/JCI109018. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Punsalang A. P., Jr, Sawyer W. D. Role of pili in the virulence of Neisseria gonorrhoeae. Infect Immun. 1973 Aug;8(2):255–263. doi: 10.1128/iai.8.2.255-263.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Qureshi R., Forstner G. G., Forstner J. F. Radioimmunoassay of human intestinal goblet cell mucin. Investigation of mucus from different organs and species. J Clin Invest. 1979 Nov;64(5):1149–1156. doi: 10.1172/JCI109568. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ray T. K. A modified method for the isolation of the plasma membrane from rat liver. Biochim Biophys Acta. 1970 Jan 6;196(1):1–9. doi: 10.1016/0005-2736(70)90159-8. [DOI] [PubMed] [Google Scholar]
- Rutter J. M., Burrows M. R., Sellwood R., Gibbons R. A. A genetic basis for resistance to enteric disease caused by E. coli. Nature. 1975 Sep 11;257(5522):135–136. doi: 10.1038/257135a0. [DOI] [PubMed] [Google Scholar]
- Takeuchi A., Inman L. R., O'Hanley P. D., Cantey J. R., Lushbaugh W. B. Scanning and transmission electron microscopic study of Escherichia coli O15 (RDEC-1) enteric infection in rabbits. Infect Immun. 1978 Feb;19(2):686–694. doi: 10.1128/iai.19.2.686-694.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson M. R., Hohmann A. W. Immunity to Escherichia coli in pigs: adhesion of enteropathogenic Escherichia coli to isolated intestinal epithelial cells. Infect Immun. 1974 Oct;10(4):776–782. doi: 10.1128/iai.10.4.776-782.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]