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. 1980 Jul;29(1):108–113. doi: 10.1128/iai.29.1.108-113.1980

Detection of Escherichia coli enterotoxins in stools.

M H Merson, R H Yolken, R B Sack, J L Froehlich, H B Greenberg, I Huq, R W Black
PMCID: PMC551081  PMID: 6995331

Abstract

We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an enzyme-linked immunosorbent assay (ELISA) detected LT in 85 and 93%, respectively, of stool specimens obtained from adults with acute diarrhea from whom an LT- and ST-producing organism had been isolated. Furthermore, the ELISA assay detected LT in 8 of 35 stool specimens from which no LT-producing E. coli had been isolated. The infant mouse assay was utilized to detect ST in these stool specimens and was found to be an insensitive method, showing positive results in only 36% of the specimens from which an ST-producing organism was isolated. Further studies are warranted to determine the diagnostic value of direct detection of LT in stools, especially by the ELISA method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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