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. 1980 Jul;29(1):175–180. doi: 10.1128/iai.29.1.175-180.1980

Surface properties of Chlamydia psittaci.

D W Vance Jr, T P Hatch
PMCID: PMC551092  PMID: 7053063

Abstract

The surface properties of elementary bodies of Chlamydia psittaci were investigated by diethylaminoethyl-Sephadex chromatography, cytophoresis, partitioning in an aqueous polymer two-phase system, and hydrophobic interaction chromatography of the organism. The surface of C. psittaci was found to be hydrophobic and negatively charged at pH 7 and to have an isoelectric point of about pH 5. Reagents which block free carboxyl groups altered the surface charge of C. psittaci and caused the organism to agglomerate. The possible significance of hydrophobicity and surface charge on the ingestion of C. psittaci by host cells is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Byrne G. I., Moulder J. W. Parasite-specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells. Infect Immun. 1978 Feb;19(2):598–606. doi: 10.1128/iai.19.2.598-606.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cunningham R. K., Söderström T. O., Gillman C. F., van Oss C. J. Phagocytosis as a surface phenomenon. V. Contact angles and phagocytosis of rough and smooth strains of Salmonella typhimurium, and the influence of specific antiserum. Immunol Commun. 1975;4(5):429–442. doi: 10.3109/08820137509057331. [DOI] [PubMed] [Google Scholar]
  4. Gregory W. W., Byrne G. I., Gardner M., Moulder J. W. Cytochalasin B does not inhibit ingestion of Chlamydia psittaci by mouse fibroblasts (L cells) and mouse peritoneal macrophages. Infect Immun. 1979 Jul;25(1):463–466. doi: 10.1128/iai.25.1.463-466.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hatch T. P. Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. Infect Immun. 1975 Jul;12(1):211–220. doi: 10.1128/iai.12.1.211-220.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hatch T. P. Utilization of L-cell nucleoside triphosphates by Chlamydia psittaci for ribonucleic acid synthesis. J Bacteriol. 1975 May;122(2):393–400. doi: 10.1128/jb.122.2.393-400.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heckels J. E., Blackett B., Everson J. S., Ward M. E. The influence of surface charge on the attachment of Neisseria gonorrhoeae to human cells. J Gen Microbiol. 1976 Oct;96(2):359–364. doi: 10.1099/00221287-96-2-359. [DOI] [PubMed] [Google Scholar]
  8. Hoare D. G., Koshland D. E., Jr A method for the quantitative modification and estimation of carboxylic acid groups in proteins. J Biol Chem. 1967 May 25;242(10):2447–2453. [PubMed] [Google Scholar]
  9. Kihlström E., Edebo L. Association of viable and inactivated Salmonella typhimurium 395 MS and MR 10 with HeLa cells. Infect Immun. 1976 Oct;14(4):851–857. doi: 10.1128/iai.14.4.851-857.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kraaipoel R. J., van Duin A. M. Isoelectric focusing of Chlamydia trachomatis. Infect Immun. 1979 Nov;26(2):775–778. doi: 10.1128/iai.26.2.775-778.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LITWIN J. The growth cycle of the psittacosis group of micro-organisms. J Infect Dis. 1959 Sep-Oct;105:129–160. doi: 10.1093/infdis/105.2.129. [DOI] [PubMed] [Google Scholar]
  12. Lepinay A., Robineaux R., Orfila J., Moncel C., Coet H., Boutry J. M. Analyse en microcinématographie à contraste de phase du developement intracellulaire de Chlamydia psittaci. Arch Gesamte Virusforsch. 1971;35(2):161–176. [PubMed] [Google Scholar]
  13. Magnusson K. E., Stendahl O., Tagesson C., Edebo L., Johansson G. The tendency of smooth and rough Salmonella typhimurium bacteria and lipopolysaccharide to hydrophobic and ionic interaction, as studied in aqueous polymer two-phase systems. Acta Pathol Microbiol Scand B. 1977 Jun;85(3):212–218. doi: 10.1111/j.1699-0463.1977.tb01698.x. [DOI] [PubMed] [Google Scholar]
  14. Sarov I., Becker Y. Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in purified trachoma elementary bodies: effect of sodium chloride on ribonucleic acid transcription. J Bacteriol. 1971 Sep;107(3):593–598. doi: 10.1128/jb.107.3.593-598.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seed T. M., Kreier J. P. Surface properties of extracellular malaria parasites: electrophoretic and lectin-binding characteristics. Infect Immun. 1976 Dec;14(6):1339–1347. doi: 10.1128/iai.14.6.1339-1347.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smyth C. J., Jonsson P., Olsson E., Soderlind O., Rosengren J., Hjertén S., Wadström T. Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography. Infect Immun. 1978 Nov;22(2):462–472. doi: 10.1128/iai.22.2.462-472.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stendahl O., Edebo L. Phagocytosis of mutants of Salmonella typhimurium by rabbit polymorphonuclear cells. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(4):481–488. doi: 10.1111/j.1699-0463.1972.tb00169.x. [DOI] [PubMed] [Google Scholar]
  18. Stendahl O., Normann B., Edebo L. Influence of O and K antigens on the surface properties of Escherichia coli in relation to phagocytosis. Acta Pathol Microbiol Scand B. 1979 Apr;87B(2):85–91. doi: 10.1111/j.1699-0463.1979.tb02408.x. [DOI] [PubMed] [Google Scholar]
  19. Stendahl O., Tagesson C., Edebo M. Partition of Salmonella typhimurium in a two-polymer acqueous phase system in relation to liability to phagocytosis. Infect Immun. 1973 Jul;8(1):36–41. doi: 10.1128/iai.8.1.36-41.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Van Oss C. J., Gillman C. F. Phagocytosis as a surface phenomenon. Contact angles and phagocytosis of non-opsonized bacteria. J Reticuloendothel Soc. 1972 Sep;12(3):283–292. [PubMed] [Google Scholar]
  21. Weiss E., Wilson N. N. Role of exogenous adenosine triphosphate in catabolic and synthetic activities of Chlamydia psittaci. J Bacteriol. 1969 Feb;97(2):719–724. doi: 10.1128/jb.97.2.719-724.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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