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. 1980 Aug;29(2):401–407. doi: 10.1128/iai.29.2.401-407.1980

Immunity to pathogenic free-living amoebae: role of humoral antibody.

R T Cursons, T J Brown, E A Keys, K M Moriarty, D Till
PMCID: PMC551132  PMID: 7216418

Abstract

Pathogenic free-living amoebae are common in nature, but few clinical infections by these amoebae have been reported. This has prompted studies of host susceptibility factors in humans. A survey of normal human sera from three New Zealand Health Districts was made; antibodies to pathogenic free-living amoebae were found in all sera, with titers ranging from 1:5 to 1:20 for Naegleria spp. and from 1:20 to 1:80 for Acanthamoeba spp. The antibodies belonged mainly to immunoglobulin G and immunoglobulin M classes. The presence of a specific neutralizing factor against Acanthamoeba spp. but not Naegleria spp. was demonstrated. Possible protective mechanisms are discussed.

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Selected References

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  1. Boyden S. V. Natural antibodies and the immune response. Adv Immunol. 1966;5:1–28. doi: 10.1016/s0065-2776(08)60271-0. [DOI] [PubMed] [Google Scholar]
  2. Brown T. J., Cursons R. T. Pathogenic free-living amebae (PFLA) from frozen swimming areas in Oslo, Norway. Scand J Infect Dis. 1977;9(3):237–240. doi: 10.3109/inf.1977.9.issue-3.16. [DOI] [PubMed] [Google Scholar]
  3. CHANG R. S., OWENS S. PATTERNS OF "LIPOVIRUS" ANTIBODY IN HUMAN POPULATIONS. J Immunol. 1964 Feb;92:313–319. [PubMed] [Google Scholar]
  4. Carter R. F. Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it. J Pathol. 1970 Apr;100(4):217–244. doi: 10.1002/path.1711000402. [DOI] [PubMed] [Google Scholar]
  5. Carter R. F. Primary amoebic meningo-encephalitis. An appraisal of present knowledge. Trans R Soc Trop Med Hyg. 1972;66(2):193–213. doi: 10.1016/0035-9203(72)90147-2. [DOI] [PubMed] [Google Scholar]
  6. Cerva L., Serbus C., Skocil V. Isolation of limax amoebae from the nasal mucosa of man. Folia Parasitol (Praha) 1973;20(1):97–103. [PubMed] [Google Scholar]
  7. Culbertson C. G. The pathogenicity of soil amebas. Annu Rev Microbiol. 1971;25:231–254. doi: 10.1146/annurev.mi.25.100171.001311. [DOI] [PubMed] [Google Scholar]
  8. Cursons R. T., Brown T. J., Bruns B. J., Taylor D. E. Primary amoebic mengingoencephalitis contracted in a thermal tributary of the Waikato River--Taupo: a case report. N Z Med J. 1976 Dec 22;84(578):479–481. [PubMed] [Google Scholar]
  9. Cursons R. T., Brown T. J., Keys E. A. Immunity to pathogenic free-living amoebae. Lancet. 1977 Oct 22;2(8043):875–876. doi: 10.1016/s0140-6736(77)90812-1. [DOI] [PubMed] [Google Scholar]
  10. Cursons R. T., Brown T. J., Keys E. A. Virulence of pathogenic free-living amebae. J Parasitol. 1978 Aug;64(4):744–745. [PubMed] [Google Scholar]
  11. Cursons R. T., Brown T. J. Use of cell cultures as an indicator of pathogenicity of free-living amoebae. J Clin Pathol. 1978 Jan;31(1):1–11. doi: 10.1136/jcp.31.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Edwards J. H., Griffiths A. J., Mullins J. Protozoa as sources of antigen in 'humidifier fever'. Nature. 1976 Dec 2;264(5585):438–439. doi: 10.1038/264438a0. [DOI] [PubMed] [Google Scholar]
  13. Ferrante A., Thong Y. H. Antibody induced capping and endocytosis of surface antigens in Naegleria fowleri. Int J Parasitol. 1979 Dec;9(6):599–601. doi: 10.1016/0020-7519(79)90018-3. [DOI] [PubMed] [Google Scholar]
  14. Hadaś E., Kasprzak W., Mazur T. Electrophoretic characterization of small free-living Amoebae. Tropenmed Parasitol. 1977 Mar;28(1):35–43. [PubMed] [Google Scholar]
  15. Haggerty R. M., John D. T. Innate resistance of mice to experimental infection with Naegleria fowleri. Infect Immun. 1978 Apr;20(1):73–77. doi: 10.1128/iai.20.1.73-77.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. John D. T., Weik R. R., Adams A. C. Immunization of mice against Naegleria fowleri infection. Infect Immun. 1977 Jun;16(3):817–820. doi: 10.1128/iai.16.3.817-820.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kadlec V., Cerva L., Skvárová J. Virulent Naegleria fowleri in an indoor swimming pool. Science. 1978 Sep 15;201(4360):1025–1025. doi: 10.1126/science.684423. [DOI] [PubMed] [Google Scholar]
  18. Martinez A. J., Markowitz S. M., Duma R. J. Experimental pneumonitis and encephalitis caused by acanthamoeba in mice: pathogenesis and ultrastructural features. J Infect Dis. 1975 Jun;131(6):692–699. doi: 10.1093/infdis/131.6.692. [DOI] [PubMed] [Google Scholar]
  19. Michael J. G. Natural antibodies. Curr Top Microbiol Immunol. 1969;48:43–62. doi: 10.1007/978-3-642-46163-7_3. [DOI] [PubMed] [Google Scholar]
  20. O'Dell W. D., Stevens A. R. Quantitative growth of Naegleria in axenic culture. Appl Microbiol. 1973 Apr;25(4):621–627. doi: 10.1128/am.25.4.621-627.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rifkin M. R. Trypanosoma brucei: some properties of the cytotoxic reaction induced by normal human serum. Exp Parasitol. 1978 Dec;46(2):189–206. doi: 10.1016/0014-4894(78)90131-5. [DOI] [PubMed] [Google Scholar]
  23. Stevens A. R., O'Dell W. D. The influence of growth medium on axenic cultivation of virulent and avirulent Acanthamoeba. Proc Soc Exp Biol Med. 1973 Jun;143(2):474–478. doi: 10.3181/00379727-143-37346. [DOI] [PubMed] [Google Scholar]
  24. Stevens A. R., Tyndall R. L., Coutant C. C., Willaert E. Isolation of the etiological agent of primary amoebic meningoencephalitis from artifically heated waters. Appl Environ Microbiol. 1977 Dec;34(6):701–705. doi: 10.1128/aem.34.6.701-705.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tew J. G., Burmeister J., Greene E. J., Pflaumer S. K., Goldstein J. A radioimmunoassay for human antibody specific for microbial antigens. J Immunol Methods. 1977;14(3-4):231–241. doi: 10.1016/0022-1759(77)90133-8. [DOI] [PubMed] [Google Scholar]
  26. Thong Y. H., Ferrante A., Shepherd C., Rowan-Kelly B. Resistance of mice to Naegleria meningoencephalitis transferred by immune serum. Trans R Soc Trop Med Hyg. 1978;72(6):650–652. doi: 10.1016/0035-9203(78)90025-1. [DOI] [PubMed] [Google Scholar]
  27. Visvesvara G. S., Jones D. B., Robinson N. M. Isolation, identification, and biological characterization of Acanthamoeba polyphaga from a human eye. Am J Trop Med Hyg. 1975 Sep;24(5):784–790. doi: 10.4269/ajtmh.1975.24.784. [DOI] [PubMed] [Google Scholar]
  28. Wellings F. M., Amuso P. T., Chang S. L., Lewis A. L. Isolation and identification of pathogenic Naegleria from Florida lakes. Appl Environ Microbiol. 1977 Dec;34(6):661–667. doi: 10.1128/aem.34.6.661-667.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. de Jonckheere J., Voorde H. The distribution of Naegleria fowleri in man-made thermal waters. Am J Trop Med Hyg. 1977 Jan;26(1):10–15. doi: 10.4269/ajtmh.1977.26.10. [DOI] [PubMed] [Google Scholar]

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