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. 1990 Dec;87(23):9133–9137. doi: 10.1073/pnas.87.23.9133

Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions.

A Matsumoto 1, M Naito 1, H Itakura 1, S Ikemoto 1, H Asaoka 1, I Hayakawa 1, H Kanamori 1, H Aburatani 1, F Takaku 1, H Suzuki 1, et al.
PMCID: PMC55118  PMID: 2251254

Abstract

Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), alpha-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.

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