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. 1980 Sep;29(3):1096–1101. doi: 10.1128/iai.29.3.1096-1101.1980

Effects of cigarette smoke and its constituents on the adherence of polymorphonuclear leukocytes.

R B Bridges, L Hsieh, D G Haack
PMCID: PMC551244  PMID: 7429629

Abstract

The in vitro effects of the water-soluble fraction of whole cigarette smoke (WSF) and two alpha, beta-unsaturated aldehydes of cigarette smoke (acrolein and crotonaldehyde) on polymorphonuclear leukocyte (PMNL) adherence were determined with nylon fiber columns. Each of these cigarette smoke constituents caused a dose-dependent inhibition of PMNL adherence. However, at least fivefold higher concentrations of these agents were necessary to inhibit adherence as compared with those necessary to achieve the same level of inhibition of PMNL chemotaxis. Furthermore, inhibition of adherence by WSF could be differentiated from its effects on chemotaxis in that reduced glutathione completely protected chemotaxis from the effects of WSF but only afforded partial protection to PMNL adherence. These data suggest that the inhibitory effects of WSF, acrolein, and crotonaldehyde on PMNL chemotaxis are not due to their inhibition of adherence. Finally, although PMNL adherence is considered to be an integral part of the chemotactic mechanism, differentiation between these two PMNL functions may be possible, since some inhibitors of chemotaxis do not have corresponding inhibitory effects on adherence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON F., Jr, LANCASTER M. G. STUDIES ON FACTORS WHICH INFLUENCE THE ADHESIVENESS OF LEUKOCYTES IN VITRO. Ann N Y Acad Sci. 1964 Aug 27;116:936–944. doi: 10.1111/j.1749-6632.1964.tb52559.x. [DOI] [PubMed] [Google Scholar]
  2. Bridges R. B., Kraal J. H., Huang L. J., Chancellor B. M. Effects of tobacco smoke on chemotaxis and glucose metabolism of polymorphonuclear leukocytes. Infect Immun. 1977 Jan;15(1):115–123. doi: 10.1128/iai.15.1.115-123.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bridges R. B., Kraal J. H., Huang L. J., Chancellor M. B. Effects of cigarette smoke components on in vitro chemotaxis of human polymorphonuclear leukocytes. Infect Immun. 1977 Apr;16(1):240–248. doi: 10.1128/iai.16.1.240-248.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryant R. E., DesPrez R. M., VanWay M. H., Rogers D. E. Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration, and phagocytosis on leukocyte migration, adhesiveness, and aggregation. J Exp Med. 1966 Sep 1;124(3):483–499. doi: 10.1084/jem.124.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  6. Clark R. A., Kimball H. R. Defective granulocyte chemotaxis in the Chediak-Higashi syndrome. J Clin Invest. 1971 Dec;50(12):2645–2652. doi: 10.1172/JCI106765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Corberand J., Nguyen F., Do A. H., Dutau G., Laharrague P., Fontanilles A. M., Gleizes B. Effect of tobacco smoking on the functions of polymorphonuclear leukocytes. Infect Immun. 1979 Mar;23(3):577–581. doi: 10.1128/iai.23.3.577-581.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Corre F., Lellouch J., Schwartz D. Smoking and leucocyte-counts. Results of an epidemiological survey. Lancet. 1971 Sep 18;2(7725):632–634. [PubMed] [Google Scholar]
  9. Friedman G. D., Siegelaub A. B., Seltzer C. C., Feldman R., Collen M. F. Smoking habits and the leukocyte count. Arch Environ Health. 1973 Mar;26(3):137–143. doi: 10.1080/00039896.1973.10666241. [DOI] [PubMed] [Google Scholar]
  10. Garvin J. E. Effects of divalent cations on adhesiveness of rat polymorphonuclear neutrophils in vitro. J Cell Physiol. 1968 Dec;72(3):197–212. doi: 10.1002/jcp.1040720307. [DOI] [PubMed] [Google Scholar]
  11. Keller H. U., Hess M. W., Cottier H. Physiology of chemotaxis and random motility. Semin Hematol. 1975 Jan;12(1):47–57. [PubMed] [Google Scholar]
  12. Mac Gregor R. R. Granulocyte adherence changes induced by hemodialysis, endotoxin, epinephrine, and glucocorticoids. Ann Intern Med. 1977 Jan;86(1):35–39. doi: 10.7326/0003-4819-86-1-35. [DOI] [PubMed] [Google Scholar]
  13. MacGregor R. R., Macarak E. J., Kefalides N. A. Comparative adherence of granulocytes to endothelial monolayers and nylon fiber. J Clin Invest. 1978 Mar;61(3):697–702. doi: 10.1172/JCI108981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MacGregor R. R., Spagnuolo P. J., Lentnek A. L. Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system. N Engl J Med. 1974 Sep 26;291(13):642–646. doi: 10.1056/NEJM197409262911302. [DOI] [PubMed] [Google Scholar]
  15. Noble R. C., Penny B. B. Comparison of leukocyte count and function in smoking and nonsmoking young men. Infect Immun. 1975 Sep;12(3):550–555. doi: 10.1128/iai.12.3.550-555.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Flaherty J. T., Kreutzer D. L., Ward P. A. Chemotactic factor influences on the aggregation, swelling, and foreign surface adhesiveness of human leukocytes. Am J Pathol. 1978 Mar;90(3):537–550. [PMC free article] [PubMed] [Google Scholar]
  17. O'Flaherty J. T., Kreutzer D. L., Ward P. A. The influence of chemotactic factors on neutrophil adhesiveness. Inflammation. 1978 Mar;3(1):37–48. doi: 10.1007/BF00917320. [DOI] [PubMed] [Google Scholar]
  18. TENNANT J. R. EVALUATION OF THE TRYPAN BLUE TECHNIQUE FOR DETERMINATION OF CELL VIABILITY. Transplantation. 1964 Nov;2:685–694. doi: 10.1097/00007890-196411000-00001. [DOI] [PubMed] [Google Scholar]

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