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. 1980 Sep;29(3):1190–1192. doi: 10.1128/iai.29.3.1190-1192.1980

Bactericidal effect of hydrogen peroxide is prevented by the lactoperoxidase-thiocyanate system under anaerobic conditions.

J Carlsson
PMCID: PMC551257  PMID: 7429633

Abstract

Streptococcus sanguis and Peptostreptococcus anaerobius were exposed to various combinations of the components of the lactoperoxidase-thiocyanate-hydrogen peroxide system. The bactericidal effect of hydrogen peroxide was prevented under anaerobic conditions by lactoperoxidase together with thiocyanate, but not by lactoperoxidase or thiocyanate alone. Thiocyanate was effective already at a molar ratio to hydrogen peroxide of 1:100.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aune T. M., Thomas E. L. Accumulation of hypothiocyanite ion during peroxidase-catalyzed oxidation of thiocyanate ion. Eur J Biochem. 1977 Oct 17;80(1):209–214. doi: 10.1111/j.1432-1033.1977.tb11873.x. [DOI] [PubMed] [Google Scholar]
  2. Björck L. Antibacterial effect of the lactoperoxidase system on psychotrophic bacteria in milk. J Dairy Res. 1978 Feb;45(1):109–118. doi: 10.1017/s0022029900016253. [DOI] [PubMed] [Google Scholar]
  3. Carlsson J., Carpenter V. S. The recA+ gene product is more important than catalase and superoxide dismutase in protecting Escherichia coli against hydrogen peroxide toxicity. J Bacteriol. 1980 Apr;142(1):319–321. doi: 10.1128/jb.142.1.319-321.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlsson J., Granberg G. P., Nyberg G. K., Edlund M. B. Bactericidal effect of cysteine exposed to atmospheric oxygen. Appl Environ Microbiol. 1979 Mar;37(3):383–390. doi: 10.1128/aem.37.3.383-390.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoogendoorn H., Piessens J. P., Scholtes W., Stoddard L. A. Hypothiocyanite ion; the inhibitor formed by the system lactoperoxidase-thiocyanate-hydrogen peroxide. I. Identification of the inhibiting compound. Caries Res. 1977;11(2):77–84. doi: 10.1159/000260252. [DOI] [PubMed] [Google Scholar]
  6. KLEBANOFF S. J., LUEBKE R. G. THE ANTILACTOBACILLUS SYSTEM OF SALIVA. ROLE OF SALIVARY PEROXIDASE. Proc Soc Exp Biol Med. 1965 Feb;118:483–486. doi: 10.3181/00379727-118-29882. [DOI] [PubMed] [Google Scholar]
  7. Mickelson M. N. Antibacterial action of lactoperoxidase-thiocyanate-hydrogen peroxide on Streptococcus agalactiae. Appl Environ Microbiol. 1979 Nov;38(5):821–826. doi: 10.1128/aem.38.5.821-826.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mickelson M. N. Glucose transport in Streptococcus agalactiae and its inhibition by lactoperoxidase-thiocyanate-hydrogen peroxide. J Bacteriol. 1977 Nov;132(2):541–548. doi: 10.1128/jb.132.2.541-548.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pruitt K. M., Adamson M., Arnold R. Lactoperoxidase binding to streptococci. Infect Immun. 1979 Jul;25(1):304–309. doi: 10.1128/iai.25.1.304-309.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tenovuo J. Formation of the bacterial inhibitor, hypothiocyanite ion, by cell-bound lactoperoxidase. Caries Res. 1979;13(3):137–143. doi: 10.1159/000260393. [DOI] [PubMed] [Google Scholar]
  11. Thomas E. L., Aune T. M. Lactoperoxidase, peroxide, thiocyanate antimicrobial system: correlation of sulfhydryl oxidation with antimicrobial action. Infect Immun. 1978 May;20(2):456–463. doi: 10.1128/iai.20.2.456-463.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yamada T., Carlsson J. Regulation of lactate dehydrogenase and change of fermentation products in streptococci. J Bacteriol. 1975 Oct;124(1):55–61. doi: 10.1128/jb.124.1.55-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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