Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Oct;30(1):17–22. doi: 10.1128/iai.30.1.17-22.1980

Lymphocyte blastogenesis and interferon production in adult human leukocyte cultures stimulated with cytomegalovirus antigens.

S E Starr, B Dalton, T Garrabrant, K Paucker, S A Plotkin
PMCID: PMC551270  PMID: 6160103

Abstract

Lymphocyte blastogenesis and interferon production were measured in adult human leukocyte cultures stimulated with purified or crude cytomegalovirus antigens. Leukocytes from seropositive adults underwent blastogenesis when stimulated with purified or crude Towne strain antigen, whereas neither antigen stimulated blastogenesis in cultures from seronegative donors. The concentrations of antigens yielding maximal blastogenesis varied among the individuals tested. When cultures from seropositive individuals were stimulated with antigens prepared from three different CMV strains--AD-169, Towne, and Davis--comparable levels of blastogenesis were detected. Type 1 interferon was detected in supernatants of cultures stimulated with crude antigens regardless of the immune status of the donor. In contrast, when purified antigen was used as the stimulant, only cultures obtained from seropositive individuals produced detectable levels of interferon, which appeared to be predominantly type 2 or immune interferon.

Full text

PDF
17

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutner K. R., Morag A., Deibel R., Morag B., Raiken D., Ogra P. L. Strain-specific local and systemic cell-mediated immune responses to cytomegalovirus in humans. Infect Immun. 1978 Apr;20(1):82–87. doi: 10.1128/iai.20.1.82-87.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gehrz R. C., Marker S. C., Knorr S. O., Kalis J. M., Balfour H. H., Jr Specific cell-mediated immune defect in active cytomegalovirus infection of young children and their mothers. Lancet. 1977 Oct 22;2(8043):844–847. doi: 10.1016/s0140-6736(77)90782-6. [DOI] [PubMed] [Google Scholar]
  3. Havell E. A., Hayes T. G., Vilcek J. Synthesis of two distinct interferons by human fibroblasts. Virology. 1978 Aug;89(1):330–334. doi: 10.1016/0042-6822(78)90068-5. [DOI] [PubMed] [Google Scholar]
  4. Levin M. J., Parkman R., Oxman M. N., Rappeport J. M., Simpson M., Leary P. L. Proliferative and interferon responses by peripheral blood mononuclear cells after bone marrow transplantation in humans. Infect Immun. 1978 Jun;20(3):678–684. doi: 10.1128/iai.20.3.678-684.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Linnemann C. C., Jr, Kauffman C. A., First M. R., Schiff G. M., Phair J. P. Cellular immune response to cytomegalovirus infection after renal transplantation. Infect Immun. 1978 Oct;22(1):176–180. doi: 10.1128/iai.22.1.176-180.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mogensen K. E., Pyhälä L., Cantell K. Raising antibodies to human leukocyte interferon. Acta Pathol Microbiol Scand B. 1975 Oct;83(5):443–450. doi: 10.1111/j.1699-0463.1975.tb00123.x. [DOI] [PubMed] [Google Scholar]
  7. Moller-Larsen A., Andersen H. K., Heron I., Sarov I. In vitro stimulation of human lymphocytes by purified cytomegalovirus. Intervirology. 1975;6(4-5):249–257. doi: 10.1159/000149479. [DOI] [PubMed] [Google Scholar]
  8. Paucker K., Dalton B. J., Ogburn C. A., Törmä E. Multiple active sites on human interferons. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4587–4591. doi: 10.1073/pnas.72.11.4587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Plotkin S. A., Furukawa T., Zygraich N., Huygelen C. Candidate cytomegalovirus strain for human vaccination. Infect Immun. 1975 Sep;12(3):521–527. doi: 10.1128/iai.12.3.521-527.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pollard R. B., Rand K. H., Arvin A. M., Merigan T. C. Cell-mediated immunity of cytomegalovirus infection in normal subjects and cardiac transplant patients. J Infect Dis. 1978 May;137(5):541–549. doi: 10.1093/infdis/137.5.541. [DOI] [PubMed] [Google Scholar]
  11. Rasmussen L., Merigan T. C. Role of T lymphocytes in cellular immune responses during herpes simplex virus infection in humans. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3957–3961. doi: 10.1073/pnas.75.8.3957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reynolds D. W., Dean P. H., Pass R. F., Alford C. A. Specific cell-mediated immunity in children with congenital and neonatal cytomegalovirus infection and their mothers. J Infect Dis. 1979 Oct;140(4):493–499. doi: 10.1093/infdis/140.4.493. [DOI] [PubMed] [Google Scholar]
  13. Rytel M. W., Aguilar-Torres F. G., Balay J., Heim L. R. Assessment of the status of cell-mediated immunity in cytomegalovirus-infected renal allograft recipients. Cell Immunol. 1978 Apr;37(1):31–40. doi: 10.1016/0008-8749(78)90171-5. [DOI] [PubMed] [Google Scholar]
  14. Starr S. E., Tolpin M. D., Friedman H. M., Paucker K., Plotkin S. A. Impaired cellular immunity to cytomegalovirus in congenitally infected children and their mothers. J Infect Dis. 1979 Oct;140(4):500–505. doi: 10.1093/infdis/140.4.500. [DOI] [PubMed] [Google Scholar]
  15. Ten Napel C. H., The T. H., Bijker J., De Gast G. C., Langenhuysen M. M. Cytomegalovirus-directed lymphocyte reactivity in healthy adults tested by a CMV-induced lymphocyte transformation test. Clin Exp Immunol. 1977 Jul;29(1):52–60. [PMC free article] [PubMed] [Google Scholar]
  16. Valle M. J., Jordan G. W., Haahr S., Merigan T. C. Characteristics of immune interferon produced by human lymphocyte cultures compared to other human interferons. J Immunol. 1975 Jul;115(1):230–233. [PubMed] [Google Scholar]
  17. Waner J. L., Budnick J. E. Blastogenic response of human lymphocytes to human cytomegalovirus. Clin Exp Immunol. 1977 Oct;30(1):44–49. [PMC free article] [PubMed] [Google Scholar]
  18. Waner J. L., Weller T. H. Analysis of antigenic diversity among human cytomegaloviruses by kinetic neutralization tests with high-titered rabbit antisera. Infect Immun. 1978 Jul;21(1):151–157. doi: 10.1128/iai.21.1.151-157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wentworth B. B., French L. Plaque assay of cytomegalovirus strains of human origin. Proc Soc Exp Biol Med. 1970 Nov;135(2):253–258. doi: 10.3181/00379727-135-35031. [DOI] [PubMed] [Google Scholar]
  20. Zaia J. A., Leary P. L., Levin M. J. Specificity of the blastogenic response of human mononuclear cells to herpesvirus antigens. Infect Immun. 1978 Jun;20(3):646–651. doi: 10.1128/iai.20.3.646-651.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES