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. 1980 Nov;30(2):342–348. doi: 10.1128/iai.30.2.342-348.1980

Comparison of human rotaviruses isolated in Mexico City and in Santiago, Chile, by electrophoretic migration of their double-stranded ribonucleic acid genome segments.

R T Espejo, L F Avendaño, O Muñoz, P Romero, J G Eternod, S Lopez, J Moncaya
PMCID: PMC551316  PMID: 6254887

Abstract

During the period October to December 1979, rotaviruses were obtained from infants and young children hospitalized with acute gastroenteritis in Mexico City and were compared by analysis of the migration of their double-stranded ribonucleic acid (RNA) genome segments in gel electrophoresis. Comparison of the results of this analysis and of those of similar studies carried out in 1977 and 1978 showed that the two rotavirus electropherotypes designated 2s and 21 have been continuously present and that the proportion in which these two types have been found in hospitalized patients has varied greatly year to year. The RNAs from rotaviruses 2s and 21 differed in the electrophoretic migraton of at least eight genome segments. However, RNAs from virus assigned to the same electrophoreotypes were not necessarily identical: on the basis of small but significant differences in the migration of segment 7, 8, or 9, isolates of types 2s and 21 could be assigned to two and three different subtypes, respectively. Human rotaviruses obtained in a distant geographical region, Santiago, Chile, in July 1979 had RNA electrophoretic patterns similar to that of electropherotype 21 but different from it in the migration of one or two of the larger RNA segments.

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Selected References

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  1. Espejo R. T., Calderon E., Gonzalez N. Distinct reovirus-like agents associated with acute infantile gastroenteritis. J Clin Microbiol. 1977 Nov;6(5):502–506. doi: 10.1128/jcm.6.5.502-506.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Espejo R. T., Calderón E., González N., Salomon A., Martuscelli A., Romero P. Presence of two distinct types of rotavirus in infants and young children hospitalized with acute gastroenteritis in Mexico City, 1977. J Infect Dis. 1979 Apr;139(4):474–477. doi: 10.1093/infdis/139.4.474. [DOI] [PubMed] [Google Scholar]
  3. Espejo R. T., Muñz O., Serafin F., Romero P. Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infect Immun. 1980 Feb;27(2):351–354. doi: 10.1128/iai.27.2.351-354.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Espejo R., Martínez E., López S., Muñoz O. Different polypeptide composition of two human rotavirus types. Infect Immun. 1980 Apr;28(1):230–237. doi: 10.1128/iai.28.1.230-237.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Flewett T. H., Thouless M. E., Pilfold J. N., Bryden A. S., Candeias J. A. More serotypes of human rotavirus. Lancet. 1978 Sep 16;2(8090):632–632. doi: 10.1016/s0140-6736(78)92854-4. [DOI] [PubMed] [Google Scholar]
  6. Gorman B. M., Taylor J. The RNA genome of Tilligerry virus. Aust J Exp Biol Med Sci. 1978 Jun;56(3):369–371. doi: 10.1038/icb.1978.40. [DOI] [PubMed] [Google Scholar]
  7. Gorman B. M., Taylor J., Walker P. J., Young P. R. The isolation of recombinants between related orbiviruses. J Gen Virol. 1978 Nov;41(2):333–342. doi: 10.1099/0022-1317-41-2-333. [DOI] [PubMed] [Google Scholar]
  8. Hrdy D. B., Rosen L., Fields B. N. Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice. J Virol. 1979 Jul;31(1):104–111. doi: 10.1128/jvi.31.1.104-111.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., van Kirk D. H., Chanock R. M., Kapikian A. Z. Differentiation of human and calf reoviruslike agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. Virology. 1976 Oct 1;74(1):86–92. doi: 10.1016/0042-6822(76)90131-8. [DOI] [PubMed] [Google Scholar]
  10. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology. 1978 Jun 15;87(2):247–255. doi: 10.1016/0042-6822(78)90130-7. [DOI] [PubMed] [Google Scholar]
  11. Laver W. G., Webster R. G. Studies on the origin of pandemic influenza. 3. Evidence implicating duck and equine influenza viruses as possible progenitors of the Hong Kong strain of human influenza. Virology. 1973 Feb;51(2):383–391. doi: 10.1016/0042-6822(73)90437-6. [DOI] [PubMed] [Google Scholar]
  12. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rodger S. M., Holmes I. H. Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J Virol. 1979 Jun;30(3):839–846. doi: 10.1128/jvi.30.3.839-846.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rodriguez W. J., Kim H. W., Brandt C. D., Yolken R. H., Arrobio J. O., Kapikian A. Z., Chanock R. M., Parrott R. H. Sequential enteric illnesses associated with different rotavirus serotypes. Lancet. 1978 Jul 1;2(8079):37–37. doi: 10.1016/s0140-6736(78)91339-9. [DOI] [PubMed] [Google Scholar]
  15. Schnagl R. D., Holmes I. H. Characteristics of the genome of human infantile enteritis virus (Rotavirus). J Virol. 1976 Jul;19(1):267–270. doi: 10.1128/jvi.19.1.267-270.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sharpe A. H., Ramig R. F., Mustoe T. A., Fields B. N. A genetic map of reovirus. 1. Correlation of genome RNAs between serotypes 1, 2, and 3. Virology. 1978 Jan;84(1):63–74. doi: 10.1016/0042-6822(78)90218-0. [DOI] [PubMed] [Google Scholar]
  17. Thouless M. E. Rotavirus polypeptides. J Gen Virol. 1979 Jul;44(1):187–197. doi: 10.1099/0022-1317-44-1-187. [DOI] [PubMed] [Google Scholar]
  18. Verly E., Cohen J. Demonstration of size variation of RNA segments between different isolates of calf rotavirus. J Gen Virol. 1977 Jun;35(3):583–586. doi: 10.1099/0022-1317-35-3-583. [DOI] [PubMed] [Google Scholar]
  19. Yolken R. H., Wyatt R. G., Zissis G., Brandt C. D., Rodriguez W. J., Kim H. W., Parrott R. H., Urrutia J. J., Mata L., Greenberg H. B. Epidemiology of human rotavirus Types 1 and 2 as studied by enzyme-linked immunosorbent assay. N Engl J Med. 1978 Nov 23;299(21):1156–1161. doi: 10.1056/NEJM197811232992103. [DOI] [PubMed] [Google Scholar]
  20. Zissis G., Lambert J. P. Different serotypes of human rotaviruses. Lancet. 1978 Jan 7;1(8054):38–39. doi: 10.1016/s0140-6736(78)90380-x. [DOI] [PubMed] [Google Scholar]

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