Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1980 Nov;30(2):349–352. doi: 10.1128/iai.30.2.349-352.1980

Comparison of quantitative and qualitative antibody-producing cell responses to lipopolysaccharide in cell walls of the bacterial form and in membranes of the protoplast L-form of Proteus mirabilis.

H Karch, K Nixdorff
PMCID: PMC551317  PMID: 7002796

Abstract

Membranes of the stable protoplast L-form of Proteus mirabilis strain VI were highly immunogenic carriers of lipopolysaccharide when compared with the immune responses to lipopolysaccharide contained in cell walls of the bacterial form of this organism.

Full text

PDF
349

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam A., Ciorbaru R., Petit J. F., Lederer E. Isolation and properties of a macromolecular, water-soluble, immuno-adjuvant fraction from the cell wall of Mycobacterium smegmatis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):851–854. doi: 10.1073/pnas.69.4.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Agarwal S. C., Ganguly N. K. Experimental oral immunization with L-forms of Vibrio cholerae. Infect Immun. 1972 Jan;5(1):31–34. doi: 10.1128/iai.5.1.31-34.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Agarwal S. C., Sundararaj T. Cell-mediated immunity after oral immunization with ribonucleic acid-protein fractions of Vibrio cholerae L-form lysates. Infect Immun. 1977 May;16(2):527–530. doi: 10.1128/iai.16.2.527-530.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dienes L. THE DEVELOPMENT OF PROTEUS CULTURES IN THE PRESENCE OF PENICILLIN. J Bacteriol. 1949 May;57(5):529–546. doi: 10.1128/jb.57.5.529-546.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Galanos C., Lüderitz O. Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Eur J Biochem. 1975 Jun;54(2):603–610. doi: 10.1111/j.1432-1033.1975.tb04172.x. [DOI] [PubMed] [Google Scholar]
  6. Gmeiner J., Martin H. H. Phospholipid and lipopolysaccharide in Proteus mirabilis and its stable protoplast L-form. Difference in content and fatty acid composition. Eur J Biochem. 1976 Aug 16;67(2):487–494. doi: 10.1111/j.1432-1033.1976.tb10714.x. [DOI] [PubMed] [Google Scholar]
  7. Gmeiner J. The isolation of two different lipopolysaccharide fractions from various Proteus mirabilis strains. Eur J Biochem. 1975 Oct 15;58(2):621–626. doi: 10.1111/j.1432-1033.1975.tb02413.x. [DOI] [PubMed] [Google Scholar]
  8. Gutman L. T., Turck M., Petersdorf R. G., Wedgwood R. J. Significance of bacterial variants in urine of patients with chronic bacteriuria. J Clin Invest. 1965 Dec;44(12):1945–1952. doi: 10.1172/JCI105300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hofschneider P. H., Martin H. H. Diversity of surface layers in L-forms of Proteus mirabilis. J Gen Microbiol. 1968 Apr;51(1):23–33. doi: 10.1099/00221287-51-1-23. [DOI] [PubMed] [Google Scholar]
  10. Jerne N. K., Nordin A. A. Plaque Formation in Agar by Single Antibody-Producing Cells. Science. 1963 Apr 26;140(3565):405–405. doi: 10.1126/science.140.3565.405. [DOI] [PubMed] [Google Scholar]
  11. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  12. MARTIN H. H. COMPOSITION OF THE MUCOPOLYMER IN CELL WALLS OF THE UNSTABLE AND STABLE L-FORM OF PROTEUS MIRABILIS. J Gen Microbiol. 1964 Sep;36:441–450. doi: 10.1099/00221287-36-3-441. [DOI] [PubMed] [Google Scholar]
  13. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  14. Martin H. H., Maskos C., Burger R. D-alanyl-D-alanine carboxypeptidase in the bacterial form and L-form of Proteus mirabilis. Eur J Biochem. 1975 Jul 1;55(2):465–473. doi: 10.1111/j.1432-1033.1975.tb02183.x. [DOI] [PubMed] [Google Scholar]
  15. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nixdorff K., Fitzer H., Gmeiner J., Martin H. H. Reconstitution of model membranes from phospholipid and outer membrane proteins of Proteus mirabilis. Role of proteins in the formation of hydrophilic pores and protection of membranes against detergents. Eur J Biochem. 1977 Nov 15;81(1):63–69. doi: 10.1111/j.1432-1033.1977.tb11927.x. [DOI] [PubMed] [Google Scholar]
  17. Ruttkowski E., Nixdorff K. Qualitative and quantitative changes in the antibody producing cell response to lipopolysaccharide induced after incorporation of the antigen into bacterial membrane phospholipid vesicles. J Immunol. 1980 Jun;124(6):2548–2551. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES