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. 2017 Jul 14;17:27. doi: 10.1186/s12898-017-0137-9

Diet segregation in American bison (Bison bison) of Yellowstone National Park (Wyoming, USA)

John L Berini 1,✉, Catherine Badgley 2
PMCID: PMC5513210  PMID: 28705156

Abstract

Background

Body size is a major factor in the nutritional ecology of ruminant mammals. Females, due to their smaller size and smaller rumen, have more rapid food-passage times than males and thereby require higher quality forage. Males are more efficient at converting high-fiber forage into usable energy and thus, are more concerned with quantity. American bison are sexually dimorphic and sexually segregate for the majority of their adult lives, and in Yellowstone National Park, they occur in two distinct subpopulations within the Northern and Central ranges. We used fecal nitrogen and stable isotopes of carbon and nitrogen from American bison to investigate sex-specific differences in diet composition, diet quality, and dietary breadth between the mating season and a time period spanning multiple years, and compared diet indicators for these different time periods between the Northern and Central ranges.

Results

During mating season, diet composition of male and female American bison differed significantly; females had higher quality diets, and males had greater dietary breadth. Over the multi-year period, females had higher quality diets and males, greater dietary breadth. Diet segregation for bison in the Central Range was more pronounced during the mating season than for the multi-year period and females had higher quality diets than males. Finally, diet segregation in the Northern Range was more pronounced during the multi-year period than during the mating season, and males had greater dietary breadth.

Conclusions

Female bison in Yellowstone National Park have higher quality diets than males, whereas males ingest a greater diversity of plants or plants parts, and bison from different ranges exhibited more pronounced diet segregation during different times. Collectively, our results suggest that diet segregation in bison of Yellowstone National Park is associated with sex-specific differences in nutritional demands. Altogether, our results highlight the importance of accounting for spatial and temporal heterogeneity when conducting dietary studies on wild ungulates.

Background

Body size is a major factor in the nutritional ecology of ruminant mammals, as mass-specific energy demands generally decrease with increasing body mass [1]. On average, the ability of ungulates to convert high-fiber, low-quality forage into usable energy increases with body size, a trend known as the Jarman–Bell principle [2, 3]. Although the Jarman–Bell principle was first used to explain dietary differences among African ruminants of varying sizes [2, 3], evidence suggests that the principles upon which the Jarman–Bell principle is founded hold true for dietary differences within species as well, and therefore, may help explain sexual segregation in sexually dimorphic ruminants [4]. Applying the Jarman–Bell principle to size-dimorphic ruminants suggests that smaller individuals should be more selective feeders and have higher quality diets than larger individuals [2–4]. In sexual segregation theory, the forage-selection hypothesis suggests that females, due to their smaller size and smaller rumen, have more rapid food-passage times than males and thereby require higher quality forage in order to maintain body weight. Males, with larger size and longer food-retention times, are more efficient at converting high-fiber forage into usable energy and thus, are more concerned with quantity [5–7]. Although numerous hypotheses have been invoked to explain sexual segregation in ruminants (for a review see [8]), this phenomenon is best characterized as the differential use of space, habitat, or food by males and females.

Sexual dimorphism and segregation in large mammals are typically associated with sex-specific differences in energetic requirements and digestion [9]. For example, the activity budget hypothesis assumes that females are less efficient at digesting forage than males, and therefore spend more time foraging, whereas males spend more time ruminating [10]. While the forage-selection hypothesis also assumes that females are less efficient at digesting forage, this hypothesis predicts group formation based on dietary preferences as opposed to activity budget. American bison (Bison bison) are highly size-dimorphic, with the average male (800 kg) weighing roughly 350 kg more than the average female (450 kg; [11]). Reproductively active females have higher energy demands than males due to gestation and lactation, with energetic requirements peaking during early to mid-summer [12, 13]. The energetic demands of males also peak during early to mid-summer, when mature individuals are replenishing energy stores in preparation for the rut, which occurs during late summer [12, 14]. During the rut, male and female bison spend approximately 1 month in large mating groups, with males tending to potential mates in an effort to maximize breeding potential [15]. Throughout the rest of the year, males and females remain segregated. Collectively, the anatomical, behavioral, and physiological differences between male and female American bison make them an ideal species for studies of diet segregation. Previous studies of sexual segregation in American bison provide evidence for both the forage-selection hypothesis and the predation-risk hypothesis. The latter assumes that females and their calves are more vulnerable to predation than males and therefore select habitats that provide increased safety over increased forage quality [5, 16].

In Yellowstone National Park (YNP), the bison population consists of two geographically and ecologically distinct subpopulations. In the Northern and Central ranges of YNP (Fig. 1), bison populations differ in fetal growth rates [17], median calving dates [17], and tooth-wear patterns [18]. Differences in mtDNA haplotype frequencies between ranges provide evidence for assortative breeding [19], and since their reintroduction in 1995, wolf predation rates on bison have been higher in the Central Range than in the Northern Range [20, 21]. Additionally, higher altitude and colder temperatures in the Central Range result in deeper snow cover, which remains significantly longer than in the Northern Range [22, 23]. The Central Range also has higher levels of geothermal activity than the Northern Range, resulting in greater abundance of winter forage [24]. However, the quality of forage located on geothermal patches tends to be of relatively low quality, with elevated levels of fluoride and silica [25]. The two ranges also differ in bedrock geology and soil type [23]. The Northern Range is dominated by andesitic bedrock, which generates soils that are richer in plant-available nutrients and organic carbon compared to soils generated by rhyolitic bedrock, which is prevalent across the Central Range [23]. Population growth of bison in YNP is thought to be contingent on herbivore density and winter-forage availability [26]. In general, Yellowstone National Park is considered to be forage-limited [26], and population densities of both elk and bison are greater on the Northern Range compared to those of the Central Range [27, 28]. Spatial and temporal variations in the biotic and abiotic environments should be reflected in the quality, abundance, and distribution of forage available to bison throughout YNP and therefore, also reflected in the composition and quality of their diets.

Fig. 1.

Fig. 1

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Distribution of carcass samples throughout the Northern and Central ranges in Yellowstone National Park (YNP). Inset map shows the location of YNP within the continental United States, as represented by the star

If different sexes face different nutritional demands, then differences in forage quality and density-dependent influences on forage availability and selection should be reflected in how similar (or different) the diets of males and females are within each range. Moreover, the degree of diet segregation between males and females within each range should vary between the mating and non-mating seasons. In the Central Range, bison and elk densities are relatively low throughout the year [27, 28]. As a result, animals in this region of the park likely experience strong density-dependent effects during late summer, when animals form large, mixed-sex mating groups. Thus, diet segregation in the Central Range should be most pronounced during this time, and should be in sharp contrast to the degree of diet segregation we might observe throughout the rest of the year. In the Northern Range, bison and elk densities are considerably higher, and as a result, animals in this region of the park likely experience strong density-dependent effects throughout the entire year. While ungulate densities in the Northern Range may also peak during the mating season, differences in the degree of diet segregation between the mating and non-mating seasons are likely less pronounced than that which we might observe on the Central Range.

Using fecal nitrogen and stable isotopes of carbon (δ13C) and nitrogen (δ15N), we evaluated whether male and female bison in YNP exhibit diet segregation and whether the degree of diet segregation varies within each range during mating season and across a time period spanning multiple years. To determine whether diet segregation of bison in YNP is associated with sex-specific nutritional demands and whether these demands are met differently in each range, we tested four hypotheses. (1) Male and female bison have distinct diet compositions during the mating season and (2) across multiple years. (3) Male and female bison from the Central Range differ more in diet composition (i.e., show more pronounced diet segregation) during the mating season than across multiple years. (4) Male and female bison from the Northern Range have different diet compositions, regardless of season. Regardless of time period or range, we expect females to have higher quality diets than males. Finally, during the mating season, males are most concerned with tending potential mates in an effort to maximize breeding potential, and thus, the variety of forages available to both males and females should be similar during this time. As a result, we expect males and females to have similar dietary breadths during the mating season, but statistically distinct dietary breadths throughout the multi-year period, regardless of range.

Methods

Study area

Yellowstone National Park occupies 891,000 ha of primarily forested habitat in northwestern Wyoming, USA, and ranges from 1500 to 3300 m in elevation [27]. During the summer months, the park is home to an estimated 3700 bison [29] that reside principally in two areas, the Central Range and the Northern Range (Fig. 1). As winter approaches, bison move to lower elevations, including the lower Yellowstone River drainage and Blacktail Deer Plateau in the Northern Range, where snow pack is shallower and the growth of spring vegetation begins earlier [30]. As noted above, distinct biotic and abiotic differences between the two ranges influence the abundance and distribution of high-quality forage for bison in YNP [23].

Analytical methods

Fecal nitrogen is an established measure of dietary quality [31], and stable isotopes, especially of carbon and nitrogen, are commonly used to investigate dietary differences both within and among mammal populations [32]. The primary source of nitrogen in feces of mammalian herbivores is dietary protein, although plant secondary metabolites that bind to proteins as well as microbial sources of nitrogen may influence estimates of protein in feces [31]. Regardless, absolute forage intake [33], forage digestibility, and dietary protein are positively correlated with fecal N in grazing ungulates [34]. Stable isotopes of carbon found in the biogenic materials of herbivores (e.g., feces and collagen) reflect the average δ13C of plants ingested and assimilated during the formation of these materials [35, 36]. Thus, δ13C is commonly used to distinguish diet compositions of large herbivores using mathematical mixing models [37]. Even without the use of mixing models, however, both the mean and variance of stable isotopes provide information about dietary habits. Differences in mean δ13C have been used to analyze population differences in diet [38], and individual variation in δ13C from biogenic materials (e.g., feces and bone) provides a measure of dietary breadth within a population. As animals ingest a greater range of plant species and plant parts, the variance of δ13C increases [39, 40]. In general, the mean δ15N of animal tissues reflects the protein content of the animal’s diet [41, 42], with a negative correlation between the nitrogen content of ingested plants and δ15N values of herbivore bone collagen (δ15Ncollagen; [43]).

Stable isotopes of carbon and nitrogen from bone collagen and feces, along with fecal N content, provide information about diet over different temporal windows. Bone formation is a relatively continuous process [44], and bone collagen has a turnover rate on the order of years [45, 46]. Thus, the isotopic composition of bone collagen reflects the average foraging trends over much of the lifetime of sampled individuals (i.e., multi-year). Stable isotope values from bison fecal samples, in contrast, reflect the isotopic composition of forage ingested during the previous 24–48 h [47].

Sample collection

We collected 60 fecal samples from bison in the Northern and Central ranges (15 males and 15 females from each range) over a period of 10 days during the mating season of early August 2009. We monitored animals along the periphery of the herd and when defecation occurred, we recorded a compass bearing in the direction of that potential sample, as well as any landmarks useful in determining the precise location of that sample (e.g., tree, bushes, flowers, mounds, etc.). No two samples were collected within close spatial proximity of one another (i.e., approximately 20 m) within the same herd, and samples were collected within 2 h of elimination. All fecal samples came from animals of reproductive age (≥6 years for males and ≥2 years for females); one fecal sample from the Central Range had to be discarded due to molding. We determined whether animals were of reproductive age based on size, head morphology, and pelage (mature males have well-defined manes that are absent on females and immature males; [48]).

The YNP scientific staff provided the locations of bison carcasses from adult animals that had died over the previous 36 months. Sample collection took place between 15 July and 7 August 2009. We collected 22 hemi-mandibles and 18 vertebrae from 40 carcasses. Twenty-five of the sampled carcasses were from the Central Range (13 females, 12 males), and 15 (11 females, 4 males) were from the Northern Range (Fig. 1). We estimated age at time of death using patterns of cheek–tooth eruption and wear [49] and the degree of fusion of suture lines on the frontal bones of the skull [50]. We determined the sex of carcasses based on sex-specific morphological differences in the skull [51] and pelvic girdle [52, 53]. For stable isotope analysis of bone collagen, we collected hemi-mandibles; when a hemi-mandible could not be located, we collected a single vertebra.

Sample preparation and analysis

We dried fecal samples at 60 °C until stable weights were achieved, then homogenized them in a SPEX Certiprep 8000D ball mill. We weighed 0.3 ± 0.05 mg of each sample and wrapped it in a Costech 5 × 9 mm tin capsule for analysis of %N and δ13C. For stable isotope analysis of bone collagen, we demineralized approximately 50 mg of bone in 0.5 N HCl for 72 h at 4 °C. We then rinsed samples with de-ionized water and placed them in 6 ml of chloroform/methanol lipid-extraction solution [54]. Finally, we lyophilized the samples for 24 h and then wrapped 0.3 ± 0.05 mg of collagen sample in a Costech 5 × 9 mm tin capsule for analysis of δ13C and δ15N [55].

Samples were analyzed in the Nadelhoffer Stable Isotope Laboratory at the University of Michigan Biological Station (Pellston, Michigan). Fecal and collagen samples were analyzed on a Thermo Delta Plus XL isotope ratio mass spectrometer via combustion in a Costech CHN analyzer, Model 4010. Analytical precision was estimated via multiple analyses of a caffeine standard (mean δ13C = −49.35, SD = 0.16; mean δ15N = −1.7‰, SD = 0.12). Stable isotope values of C and N were reported to the nearest 0.01 and 0.1‰, respectively, and fecal-N content (%Nfeces) was reported to the nearest 0.01%. Data acquisition and instrument control were accomplished using ISODAT 2.0. Isotopic values of feces and bone collagen were reported relative to international standards, Vienna Pee Dee Belemnite (VPDB) for carbon and atmospheric N for nitrogen.

Statistical analysis

We used multivariate analysis of variance (MANOVA) to determine whether diets differ throughout YNP as a function of sex and range. Specifically, we evaluated six different models (Table 1) to determine whether diets vary as a function of sex and range during the mating season (hypothesis 1, model 1) and over a multi-year period (hypothesis 2, model 2) and whether diets of males and females differ during the mating season and over a multi-year period in both the Central (hypothesis 3, models 3.1 and 3.2, respectively) and Northern ranges (hypotheses 4, models 4.1 and 4.2, respectively). For any tests resulting in P < 0.10, we applied a univariate ANOVA to determine whether any of the dependent variables were significantly influenced by our independent variables (i.e., sex and range).

Table 1.

Structure and sample size for MANOVA models

Ha x y Time period Sample size Model ID
Female Male
1 Sex δ13Cfeces, %Nfeces Mating season 30 29 1
2 Sex δ13Ccollagen, δ15Ncollagen Multi-year 24 16 2
3 Sex, CR δ13Cfeces, %Nfeces Mating season 15 14 3.1
δ13Ccollagen, δ15Ncollagen Multi-year 13 12 3.2
4 Sex, NR δ13Cfeces, %Nfeces Mating season 15 15 4.1
δ13Ccollagen, δ15Ncollagen Multi-year 11 4 4.2
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Models tested whether diet varies as a function of sex and range between mating season and a multi-year time period. To test hypotheses 3 and 4, we subset the data for each range (CR, Central Range; NR, Northern Range), and analyzed the effect of sex on proxy measures for diet within each range. Columns labeled “x” and “y” denote the independent and dependent variables, respectively, for each model

We also investigated differences in dietary breadth using Bartlett’s test for homogeneity of variance [56]. We compared variance of δ13Cfeces to determine if dietary breadth varied as a function of sex and range during the mating season and compared variance of δ13Ccollagen to determine whether dietary breadth varied as a function of sex across a multi-year time period. While our sample sizes may seem small when subdivided by sex and range, Clementz and Koch [57] reported that a sample size of n = 5 resulted in a standard error of 0.01‰ for the mean δ13C value of the study population and recommended ≥5 as suitable sample size for stable isotope studies of populations. However, one of our collagen test groups, Northern Range males, had a sample size just below this threshold. To determine the influence of this small sample size, we calculated 95% confidence intervals for each sex-range combination (Fig. 2). Additionally, we performed an F test to compare the variance of Northern Range males (n = 4) to Central Range males (n = 12) and found the variance between the two groups to be statistically indistinguishable (F = 1.3463, P = 0.6193). All statistical analyses were performed using base packages in R 3.1.2 [58].

Fig. 2.

Fig. 2

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Mean and 95% confidence intervals for each combination of sex and range for δ13C of collagen (δ13Ccollagen). Points represent mean values for δ13Ccollagen and bars represent 95% confidence intervals. Sample sizes for Northern Range bison are n = 11 for females and n = 4 for males, and sample sizes for Central Range bison are n = 13 for females and n = 12 for males

Results

Male and female bison throughout YNP had distinctly different diets during the mating season (Fig. 3; Table 2). While there was no difference in dietary breadth during this time (variance of δ13Cfeces, Table 2), mean values of δ13Cfeces were significantly different between sexes indicating distinct diet compositions. Females had greater fecal nitrogen (%Nfeces) than males (Fig. 3; Table 3), indicating higher quality diets during the mating season. Throughout the multi-year period, there was no difference in mean δ13Ccollagen (Fig. 4; Table 3) between sexes, indicating that males and females ingest similar plants or plant parts, on average. Despite having similar mean diet compositions, as a group, males had greater dietary breadth (variance of δ13Ccollagen, Table 2) during the multi-year period, whereas females had higher quality diets, as indicated by lower δ15Ncollagen (Fig. 4; Table 3).

Fig. 3.

Fig. 3

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Stable isotope values of carbon (δ13Cfeces) and nitrogen content (%Nfeces) from bison feces. Open symbols represent males, and filled symbols represent females. Bold diamonds with bars represent mean ± 1 standard deviation, while lighter diamonds represent raw data

Table 2.

Results for single-factor MANOVA tests and Bartlett’s tests for homogeneity of variance

Model ID MANOVA P K 2 Bartlett’s test
P
df F
1 1,57 8.698 <0.001* 0.008 0.931
2 1,38 2.991 0.063 7.175 0.007*
3.1 1,27 11.915 <0.001* 1.718 0.190
3.2 1,23 1.434 0.260 0.781 0.377
4.1 1,28 2.025 0.153 1.430 0.232
4.2 1,13 5.403 0.021* 5.076 0.024*
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Statistically significant results (P < 0.05) for both tests are identified with an asterisk. Bartlett’s test for homogeneity of variance was used to analyze differences in variance of δ13Cfeces (mating season) and δ13Ccollagen (multi-year)

Table 3.

Univariate ANOVA results for MANOVA tests with P < 0.10

Model ID x y Mean values df SS F P
Female Male
1 Sex δ13Cfeces −28.61 −28.36 1,57 0.904 17.570 <0.001*
% Nfeces 2.20 2.04 1,57 0.357 5.292 0.025*
2 Sex δ13Ccollagen −21.96 −21.82 1,38 0.171 1.536 0.223
δ15Ncollagen 3.94 4.46 1,38 2.583 4.905 0.033*
3.1 Sex, CR δ13Cfeces −28.76 −28.38 1,27 1.056 18.670 <0.001*
%Nfeces 2.46 2.14 1,27 0.713 20.410 <0.001*
4.2 Sex, NR δ13Ccollagen −21.88 −21.40 1,13 0.691 11.550 0.005*
δ15Ncollagen 3.66 3.82 1,13 0.076 0.228 0.641
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Significant difference in δ13C values between males and females indicates different dietary sources during each respective time period. Higher %Nfeces indicates higher quality diets during the mating season, whereas lower δ15Ncollagen indicates higher quality diet during the multi-year time period. Stable isotope values are reported to the nearest 0.01‰, and %Nfeces is reported to the nearest 0.01%. Columns labeled “x” and “y” denote the independent and dependent variables, respectively, for each model

Fig. 4.

Fig. 4

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Stable isotope values of carbon (δ13Ccollagen) and nitrogen (δ15Ncollagen) from collagen tissue of male and female bison. Open symbols represent males, and filled symbols represent females. Bold diamonds with bars represent mean ± 1 standard deviation, while lighter diamonds represent raw data

In the Central Range, male and female bison had significantly different diets during the mating season but not across the multi-year period (Table 2). Mean values of δ13Cfeces differed significantly between sexes in the Central Range, indicating distinct diet compositions during the mating season. Females had greater %Nfeces compared to males (Table 3), indicating higher quality diets; however, there was no difference in dietary breadth between sexes during this time (variance δ13Cfeces, Table 2). During the multi-year period, male and female diets in the Central Range were statistically indistinguishable in all regards, indicating diets of similar composition (δ13Ccollagen), quality (δ15Ncollagen), and breadth (variance δ13Ccollagen, Table 2).

In contrast to what we observed in the Central Range, male and female bison in the Northern Range had significantly different diets during the multi-year period but not during the mating season (Table 2). During mating season, male and female diets in the Northern Range were statistically indistinguishable in all regards, indicating diets of similar composition, quality, and breadth (Table 2). However, during the multi-year period, δ13Ccollagen differed significantly between sexes, indicating sex-specific differences in mean diet composition (Table 3). Finally, although males had greater dietary breadth (variance δ13Ccollagen, Table 2) during the multi-year period in the Northern Range, there was no difference in diet quality (δ15Ncollagen, Table 3).

Discussion

We studied whether diet segregation of bison in YNP is associated with sex-specific nutritional demands and whether these demands are met differently in each range. We tested four hypotheses and found complete or partial support for each. Specifically, we found that mean diet composition of male and female bison during the mating season differs significantly, with females having higher quality diets and males having greater dietary breadth (hypothesis 1). Further, while mean diet composition for male and female bison throughout multiple years is statistically indistinguishable, females have higher quality diets and males have greater dietary breadth (hypothesis 2). Additionally, diet segregation for bison in the Central Range was more pronounced during the mating season than across the multi-year period; while females had higher quality diets than males during this time, there was no difference in dietary breadth (hypothesis 3). Finally, diet segregation in the Northern Range was more pronounced across the multi-year period than during the mating season; while males had greater dietary breadth during this time, there was no difference in diet quality (hypothesis 4).

Our results suggest that diet segregation of bison in YNP is associated with sex-specific nutritional demands and density-dependent influences associated with meeting these demands. During mating season, the diets of male and female bison are composed of different plants or plant parts (based on differences in δ13Cfeces), with females consuming higher quality diets on average (higher %Nfeces). Although the difference in δ13Cfeces between sexes is small (0.25‰), it represents more than 20% of the total range of δ13Cfeces values. Over the multi-year time period, male and female bison, on average, consume a similar array of plants or plant parts throughout the park (based on similarity of δ13Ccollagen). Despite the similarity in average diet composition, however, females have higher quality diets (lower δ15Ncollagen) than males and appear to be more consistent in their use of available forages (lower variance in δ13Ccollagen). Collectively, these results suggest that females exhibit more selective feeding behavior throughout the majority of the year compared to males. Numerous field studies of ungulates have reported that, while different sexes may forage in the same habitats, their diets can differ significantly, with females usually selecting forage of higher quality [7, 59, 60]. For example, dietary overlap of male and female white-tailed deer decreases during periods of aggregation (e.g., mating season) compared to periods of segregation [61], and fecal nitrogen of females is greater than that of males, regardless of season [62].

Despite the fact that the two bison populations studied are separated by only tens of kilometers, we found evidence of opposing responses of sex-specific diet segregation in the two ranges. In the Central Range, diet segregation in bison was apparent during the mating season but not during the multi-year period, whereas in the Northern Range, diet segregation was apparent during the multi-year period but not during the mating season. In the Central Range, although males and females obtained a majority of their forage from different plants or plant parts during the mating season, with females ingesting higher quality forage, there was no difference in dietary breadth. Over the multi-year period, males and females from the Northern Range obtained a majority of their forage from different plants or different plant parts. While there was no sex-specific difference in dietary quality for Northern Range bison during this time, males consumed a greater diversity of dietary items compared to females. Opposing responses of diet segregation in each range may result from differences in the abundance and distribution of high-quality forage and the varying degree of competition for this forage across different ranges and different time periods.

While the Yellowstone ecosystem is considered to be forage-limited for its ungulate herbivores, competition for forage is likely greater on the Northern Range than on the Central Range [26]. The Northern Range offers high-quality forage throughout much of the year, and population densities of elk and bison are greater on the Northern Range, especially during the mating season [27, 28]. Although competition for forage peaks during the mating season for bison in both the Northern and Central ranges, range-specific differences in the availability of high-quality forage may help explain the differences in diet segregation.

Another important consideration is the abundance and distribution of potential predators. While predation rates on bison vary throughout the park, elk are the primary prey of wolves in Yellowstone. In the Northern Range, where elk are most abundant, bison make up only about 4% of wolf kills, with elk comprising approximately 80% of kills [21]. Elk are much less abundant in the Central Range, and as a result, wolf predation rates on bison are higher there than anywhere else in the park, with approximately 10% of wolf kills in this region consisting of bison and around 40% of kills consisting of elk [20]. Moreover, while bison populations without predators have been characterized by the forage selection hypothesis [5, 16], populations with predators have been characterized by the predation risk hypothesis [60], in which females select habitats that offer increased safety at the cost of decreased food quality [63, 64]. Evidence suggests that the presence of potential predators can result in decreased foraging activity [65, 66], thereby directly influencing diet quality of prey [67]. Altogether, our results suggest that bison in Yellowstone National Park are best characterized by the forage selection hypothesis.

Although we interpret our results in the context of the forage selection hypothesis, our results are also consistent with the niche trade-off framework described by Bowyer [8], in which overlap on one niche axis (e.g., space) is accompanied by avoidance on another (e.g., diet). During mating season in YNP, females outnumber males by about 2–1 [68, 69]. Thus, males may be at a competitive disadvantage for those plants that females prefer but that make up at least a portion of male diets during the rest of the year. However, intraspecific competition as a driver of diet segregation in ruminants has been rejected several times in the literature [70, 71], and testing this hypothesis is beyond the scope of this study. Although higher densities of females during the mating season may exclude males from consuming certain forages, these differences are best explained by morphological differences between sexes resulting in different physiologies and therefore different nutritional demands. Specifically, it has been hypothesized that a significant increase in the ingestion of high-quality forage by males could lead to mal-absorption, bloat, and ultimately, decreased digestive efficiency [72]. For comparison, in southern Texas, adult white-tailed deer shifted to lower quality diets as levels of intraspecific competition increased, with dietary shifts of males being more pronounced than those of females [61]. However, the authors rejected competition as a cause for sexual segregation and suggested that predator avoidance by females with young best explained the observed pattern [61]. Similarly, bighorn sheep in Sierra Nevada, California exhibited stronger niche differentiation during periods of spatial overlap than during periods of spatial separation [73]; however, the authors noted that these differences are most likely explained either by morphological differences or by how different sexes manage risk of predation [73]. It is also important to note that male ruminants tend to restrict feeding behavior during mating season [74], potentially resulting in decreased dietary breadth and lower variance of δ13C during periods of aggregation.

Finally, many studies investigating isotopic variation among conspecifics on different dietary regimes or in different regions have found only minor differences in isotope values between test groups. For example, several populations of mule deer with significant differences in fecal N content exhibited only small differences in mean δ15Nfeces, ranging from −0.54 to 1.10‰ [75]. Similarly, a study investigating the isotopic composition of hair in cattle (Bos taurus) from different confinement and dietary practices found that six of 13 test groups had mean δ13C values within a range of less than 1‰ [76]. Cattle, elk, and mule deer in Starkey Experimental Forest, Oregon, had a collective δ13C range of less than 2‰ and a collective δ15N range of less than 4‰ [39]. In YNP, Feranec reported that mean δ13C values of feces collected over a 1-month period from bison, elk, mule deer, and white-tailed deer (O. virginianus) fell within a range of less than 1‰, and mean δ13C values of collagen for bison, elk, and mule deer lay within a range of about 2‰ [77]. Moreover, of the large herbivores resident to YNP, bison had the smallest δ13C range for feces and collagen, both of which were 0.9‰. Thus, while differences in forage type, feeding strategy (e.g., browser vs. grazer), and ecological setting may result in only small differences in isotopic values, these differences may correspond to biologically significant variations in diet [78]. Finally, while some of our sample sizes may seem small for groups divided by sex and range, all but one sample size was well over the recommended threshold for stable-isotopic characterization of populations [53]. For the sample that lay below this threshold, Northern Range males, the variance is statistically indistinguishable from that of Central Range males, which has three times the number of samples. Regardless, this particular result should be considered preliminary. Also, the threshold value for sample size was determined for enamel carbonate samples, not for collagen and fecal isotopes; however, more recent studies corroborate these findings using collagen isotopes [79].

Conclusions

Ecological theory suggests that smaller-bodied, female bison should displace males from high-quality foraging habitats, since females forage in large groups and deplete resources more rapidly than do males [2, 3, 7]. Based on our results, female bison in YNP have higher quality diets than males do, suggesting that diet segregation is associated with sex-specific nutritional demands. Additionally, range-specific differences in the abundance and distribution of high-quality forage, in conjunction with seasonal variation in population density of bison and elk, may influence spatial and temporal differences in diet segregation. Altogether, our results highlight the importance of accounting for spatiotemporal heterogeneity when conducting dietary studies on wild ungulates.

Authors’ contributions

JLB and CB formulated the study idea and developed the study methods. JLB conducted fieldwork, laboratory work, and analyzed the data. JLB and CB wrote the manuscript. Both authors read and approved the final manuscript.

Acknowledgements

We thank S. Gunther, C. Hendrix, C. Curry, and B. Guild for providing logistical support and R. Wallen and K. Gunther for familiarizing us with Yellowstone National Park and its wildlife populations. We thank A. Pfrimmer for help in collecting carcass and fecal samples and J. LeMoine and M. Grant for guidance during stable isotope analysis. The suggestions of P. Myers and K. Nadelhoffer significantly improved this study. R. Feranec, D. L. Fox, and anonymous reviewers provided constructive comments on the manuscript.

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

The datasets analyzed in the current study are available from the corresponding author on reasonable request.

Ethics approval and consent to participate

All samples were collected under National Park Service Scientific Research and Collecting Permit number YELL-2009-SCI-5812. The remaining bone samples have been reposited at the University of Michigan Museum of Zoology, Ann Arbor, Michigan.

Funding

The University of Michigan provided financial support via the Rackham Graduate Student Research Grant, the Department of Ecology and Evolutionary Biology Block Grants, and the Frontiers Master’s Fellowship Program in the Department of Ecology and Evolutionary Biology.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Contributor Information

John L. Berini, Phone: 734-276-7398, Email: beri0015@umn.edu

Catherine Badgley, Email: cbadgley@umich.edu.

References

  • 1.Mysterud A. The relationship between ecological segregation and sexual body size dimorphism in large herbivores. Oecologia. 2000;124:40–54. doi: 10.1007/s004420050023. [DOI] [PubMed] [Google Scholar]
  • 2.Bell RH. A grazing ecosystem in the Serengeti. Sci Am. 1971;225:86–93. doi: 10.1038/scientificamerican0771-86. [DOI] [Google Scholar]
  • 3.Jarman P. The social organisation of antelope in relation to their ecology. Behaviour. 1974;48:215–267. doi: 10.1163/156853974X00345. [DOI] [Google Scholar]
  • 4.Perez-Barberia FJ, Pérez-Fernández E, Robertson E, Alvarez-Enriquez B. Does the Jarman–Bell principle at intra-specific level explain sexual segregation in polygynous ungulates? Sex differences in forage digestibility in Soay sheep. Oecologia. 2008;157:21–30. doi: 10.1007/s00442-008-1056-4. [DOI] [PubMed] [Google Scholar]
  • 5.Post DM, Armbrust TS, Horne EA, Goheen JR. Sexual segregation results in differences in content and quality of bison (Bos bison) diets. J Mammal. 2001;82:407–413. doi: 10.1644/1545-1542(2001)082&#x0003c;0407:SSRIDI&#x0003e;2.0.CO;2. [DOI] [Google Scholar]
  • 6.Ruckstuhl KE. Foraging behaviour and sexual segregation in bighorn sheep. Anim Behav. 1998;56:99–106. doi: 10.1006/anbe.1998.0745. [DOI] [PubMed] [Google Scholar]
  • 7.Du Toit JT. Sex differences in the foraging ecology of large mammalian herbivores. In: Ruckstuhl K, Neuhaus P, editors. Sexual segregation in vertebrates: ecology of the two sexes. Cambridge: Cambridge University Press; 2006. pp. 35–52. [Google Scholar]
  • 8.Bowyer RT. Sexual segregation in ruminants: definitions, hypotheses, and implications for conservation and management. J Mammal. 2004;85:1039–1052. doi: 10.1644/BBL-002.1. [DOI] [Google Scholar]
  • 9.Main MB, Du Toit JT. Sex differences in reproductive strategies affect habitat choice in ungulates. In: Ruckstuhl K, Neuhaus P, editors. Sexual segregation in vertebrates: ecology of the two sexes. Cambridge: Cambridge University Press; 2006. pp. 148–162. [Google Scholar]
  • 10.Ruckstuhl KE, Neuhaus P. Sexual segregation in ungulates: a comparative test of three hypotheses. Biol Rev Camb Philos Soc. 2002;77:77–96. doi: 10.1017/S1464793101005814. [DOI] [PubMed] [Google Scholar]
  • 11.Berger J, Peacock M. Variability in size–weight relationships of Bison bison. J Mammal. 1988;69:618–624. doi: 10.2307/1381357. [DOI] [Google Scholar]
  • 12.Main MB, Weckerly FW, Bleich VC. Sexual segregation in ungulates: new directions for research. J Mammal. 1996;77:449–461. doi: 10.2307/1382821. [DOI] [Google Scholar]
  • 13.Robbins C. Wildlife feeding and nutrition. Atlanta: Elsevier; 2012. [Google Scholar]
  • 14.Forsyth DM, Duncan RP, Tustin KG, Gaillard J-M. A substantial energetic cost to male reproduction in a sexually dimorphic ungulate. Ecology. 2005;86:2154–2163. doi: 10.1890/03-0738. [DOI] [Google Scholar]
  • 15.Larter NC. Diet and habitat selection of an erupting wood bison population. Master’s thesis. University of British Columbia, Vancouver; 1988.
  • 16.Mooring MS, Reisig DD, Osborne ER, Kanallakan AL, Hall BM, Schaad EW, et al. Sexual segregation in bison: a test of multiple hypotheses. Behaviour. 2005;142:897–927. doi: 10.1163/1568539055010110. [DOI] [Google Scholar]
  • 17.Gogan PJP, Podruzny KM, Olexa EM, Pac HI, Frey KL. Yellowstone bison fetal development and phenology of parturition. J Wildl Manag. 2005;69:1716–1730. doi: 10.2193/0022-541X(2005)69[1716:YBFDAP]2.0.CO;2. [DOI] [Google Scholar]
  • 18.Christianson DA, Gogan PJP, Podruzny KM, Olexa EM. Incisor wear and age in Yellowstone bison. Wildl Soc Bull. 2005;33:669–676. doi: 10.2193/0091-7648(2005)33[669:IWAAIY]2.0.CO;2. [DOI] [Google Scholar]
  • 19.Gardipee FM. Development of fecal DNA sampling methods to assess genetic population structure of Greater Yellowstone bison. Master’s thesis. The University of Montana, Missoula; 2007.
  • 20.Becker MS, Garrott RA, White PJ, Gower CN, Bergman EJ, Jaffe R. Wolf prey selection in an elk–bison system: choice or circumstance? Terr Ecol. 2008;3:305–337. doi: 10.1016/S1936-7961(08)00216-9. [DOI] [Google Scholar]
  • 21.Smith D, Stahler D, Albers E, Metz M, Williamson L, Ehlers N, et al. Yellowstone wolf project: annual report 2008 (YCR-2009-03). National Park Service, Yellowstone center for resources; 2009.
  • 22.Despain DG. Two climates of Yellowstone National Park. Proc Mont Acad Sci. 1987;47:11–19. [Google Scholar]
  • 23.Despain DG. Yellowstone vegetation: consequences of environment and history in a natural setting. Boulder: Roberts Rinehart Publishers; 1990. [Google Scholar]
  • 24.Fournier RO. Geochemistry and dynamics of the Yellowstone National Park hydrothermal system. Annu Rev Earth Planet Sci. 1989;17:13–53. doi: 10.1146/annurev.ea.17.050189.000305. [DOI] [Google Scholar]
  • 25.Garrott RA, Eberhardt LL, Otton JK, White PJ, Chaffee MA. A geochemical trophic cascade in Yellowstone’s geothermal environments. Ecosystems. 2002;5:659–666. doi: 10.1007/s10021-002-0211-8. [DOI] [Google Scholar]
  • 26.Gates CC, Stelfox B, Muhly T, Chowns T, Hudson RJ. The ecology of bison movements and distribution in and beyond Yellowstone National Park: a critical review with implications for winter use and transboundary population management. The United States National Park Service; 2005.
  • 27.Smith DW, Mech LD, Meagher M, Clark WE, Jaffe R, Phillips MK, et al. Wolf–bison interactions in Yellowstone National Park. J Mammal. 2000;81:1128–1135. doi: 10.1644/1545-1542(2000)081&#x0003c;1128:WBIIYN&#x0003e;2.0.CO;2. [DOI] [Google Scholar]
  • 28.Smith DW, Drummer TD, Murphy KM, Guernsey DS, Evans SB. Winter prey selection and estimation of wolf kill rates in Yellowstone National Park, 1995–2000. J Wildl Manag. 2004;68:153–166. doi: 10.2193/0022-541X(2004)068[0153:WPSAEO]2.0.CO;2. [DOI] [Google Scholar]
  • 29.McDonald T, Carlson E, Patterson A, Zaluski M, Jones A, McDonald K, et al. 2012 Annual report of the interagency bison management plant. 2012.
  • 30.White PJ, Wallen RL, Hallac DE, Jerrett JA, editors. Yellowstone bison—conserving an american icon in modern society. Yellowstone National Park: Yellowstone Association; 2015. [Google Scholar]
  • 31.Leslie DM, Bowyer RT, Jenks JA. Facts from feces: nitrogen still measures up as a nutritional index for mammalian herbivores. J Wildl Manag. 2008;72:1420–1433. doi: 10.2193/2007-404. [DOI] [Google Scholar]
  • 32.Cerling T, Harris J, Leakey M. Browsing and grazing in elephants: the isotope record of modern and fossil proboscideans. Oecologia. 1999;120:364–374. doi: 10.1007/s004420050869. [DOI] [PubMed] [Google Scholar]
  • 33.Stallcup OT, Davis GV, Shields L. Influence of dry matter and nitrogen intakes on fecal nitrogen losses in cattle. J Dairy Sci. 1975;58:1301–1307. doi: 10.3168/jds.S0022-0302(75)84710-2. [DOI] [PubMed] [Google Scholar]
  • 34.Lancaster RJ. Estimation of digestibility of grazed pasture from faeces nitrogen. Nature. 1949;163:330–331. doi: 10.1038/163330b0. [DOI] [PubMed] [Google Scholar]
  • 35.Cerling T, Harris J. Carbon isotope fractionation between diet and bioapatite in ungulate mammals and implications for ecological and paleoecological studies. Oecologia. 1999;120:347–363. doi: 10.1007/s004420050868. [DOI] [PubMed] [Google Scholar]
  • 36.Deniro M, Epstein S. Influence of diet on the distribution of carbon isotopes in animals. Geochim Cosmochim Acta. 1978;42:495–506. doi: 10.1016/0016-7037(78)90199-0. [DOI] [Google Scholar]
  • 37.Phillips DL. Converting isotope values to diet composition: the use of mixing models. J Mammal. 2012;93:342–352. doi: 10.1644/11-MAMM-S-158.1. [DOI] [Google Scholar]
  • 38.Angerbjörn A, Hersteinsson P, Lidén K, Nelson E. Dietary variation in arctic foxes (Alopex lagopus)—an analysis of stable carbon isotopes. Oecologia. 1994;99:226–232. doi: 10.1007/BF00627734. [DOI] [PubMed] [Google Scholar]
  • 39.Stewart KM, Bowyer RT, Kie JG, Dick BL, Ben-David M. Niche partitioning among mule deer, elk, and cattle: do stable isotopes reflect dietary niche? Ecoscience. 2003;10:297–302. doi: 10.1080/11956860.2003.11682777. [DOI] [Google Scholar]
  • 40.Newsome SD, Tinker MT, Monson DH, Oftedal OT, Ralls K, Staedler MM, et al. Using stable isotopes to investigate individual diet specialization in California sea otters (Enhydra lutris nereis) Ecology. 2009;90:961–974. doi: 10.1890/07-1812.1. [DOI] [PubMed] [Google Scholar]
  • 41.Ambrose SH. Effects of diet, climate and physiology on nitrogen isotope abundances in terrestrial foodwebs. J Archaeol Sci. 1991;18:293–317. doi: 10.1016/0305-4403(91)90067-Y. [DOI] [Google Scholar]
  • 42.Schoeninger MJ, DeNiro MJ. Nitrogen and carbon isotopic composition of bone collagen from marine and terrestrial animals. Geochim Cosmochim Acta. 1984;48:625–639. doi: 10.1016/0016-7037(84)90091-7. [DOI] [Google Scholar]
  • 43.Sealy JC, van der Merwe NJ, Thorp JAL, Lanham JL. Nitrogen isotopic ecology in southern Africa: implications for environmental and dietary tracing. Geochim Cosmochim Acta. 1987;51:2707–2717. doi: 10.1016/0016-7037(87)90151-7. [DOI] [Google Scholar]
  • 44.Glimcher MJ. Bone: nature of the calcium phosphate crystals and cellular, structural, and physical chemical mechanisms in their formation. Rev Mineral Geochem. 2006;64:223–282. doi: 10.2138/rmg.2006.64.8. [DOI] [Google Scholar]
  • 45.Koch PL. Isotopic study of the biology of modern and fossil vertebrates. In: Michener R, Lajtha K, editors. Stable isotopes in ecology and environmental science. 2. Malden: Blackwell Publishing Ltd; 2008. pp. 99–154. [Google Scholar]
  • 46.Rubenstein D, Hobson K. From birds to butterflies: animal movement patterns and stable isotopes. Trends Ecol Evol. 2004;19:256–263. doi: 10.1016/j.tree.2004.03.017. [DOI] [PubMed] [Google Scholar]
  • 47.Rutley BD, Hudson RJ. Seasonal energetic parameters of free-grazing bison (Bison bison) Can J Anim Sci. 2000;80:663–671. doi: 10.4141/A99-114. [DOI] [Google Scholar]
  • 48.Reynolds HW, Gates CC, Glaholt RD. Bison. Wild mammals of North America: biology, management, and conservation. 2. Baltimore: The Johns Hopkins University Press; 2003. pp. 1009–1060. [Google Scholar]
  • 49.Frison GC, Reher CA. Age determination of buffalo by teeth eruption and wear. Plain Anthropol. 1970;15:46–50. [Google Scholar]
  • 50.Skinner MF, Kaisen OC. The fossil bison of Alaska and preliminary revision of the genus. Bull Am Mus Nat Hist. 1947;89:1–154. [Google Scholar]
  • 51.Shackleton DM, Hills LV, Hutton DA. Aspects of variation in cranial characters of plains bison (Bison bison bison Linnaeus) from Elk Island National Park, Alberta. J Mammal. 1975;56:871–887. doi: 10.2307/1379658. [DOI] [PubMed] [Google Scholar]
  • 52.Edwards JK, Marchinton RL, Smith GF. Pelvic girdle criteria for sex determination of white-tailed deer. J Wildl Manag. 1982;46:544–547. doi: 10.2307/3808678. [DOI] [Google Scholar]
  • 53.Tyler NJC. Sexual dimorphism in the pelvic bones of Svalbard reindeer, Rangifer tarandus platyrhynchus. J Zool. 1987;213:147–152. doi: 10.1111/j.1469-7998.1987.tb03686.x. [DOI] [Google Scholar]
  • 54.Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959;37:911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  • 55.Burton RK, Koch PL. Isotopic tracking of foraging and long-distance migration in northeastern Pacific pinnipeds. Oecologia. 1999;119:578–585. doi: 10.1007/s004420050822. [DOI] [PubMed] [Google Scholar]
  • 56.Sokal RR, Rohlf FJ. Bartlett’s test of homogeneity of variances. Biometry. San Francisco: WH Freeman and Co.; 1969. pp. 370–389. [Google Scholar]
  • 57.Clementz MT, Koch PL. Differentiating aquatic mammal habitat and foraging ecology with stable isotopes in tooth enamel. Oecologia. 2001;129:461–472. doi: 10.1007/s004420100745. [DOI] [PubMed] [Google Scholar]
  • 58.R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing; 2013. http://www.R-project.org. Accessed 7 Mar 2014.
  • 59.Clutton-Brock TH, Guinness FE, Albon SD. Red deer: behavior and ecology of two sexes. Chicago: University of Chicago Press; 1982. [Google Scholar]
  • 60.Komers PE, Messier F, Gates CC. Group structure in wood bison: nutritional and reproductive determinants. Can J Zool. 1993;71:1367–1371. doi: 10.1139/z93-188. [DOI] [Google Scholar]
  • 61.Kie JG, Bowyer RT. Sexual segregation in white-tailed deer: density-dependent changes in use of space, habitat selection, and dietary niche. J Mammal. 1999;80:1004–1020. doi: 10.2307/1383271. [DOI] [Google Scholar]
  • 62.Gallina S, Sánchez-Rojas G, Buenrostro-Silva A, López-González CA. Comparison of faecal nitrogen concentration between sexes of white-tailed deer in a tropical dry forest in southern Mexico. Ethol Ecol Evol. 2015;27:103–115. doi: 10.1080/03949370.2014.891537. [DOI] [Google Scholar]
  • 63.Festa-Bianchet M. Seasonal range selection in bighorn sheep: conflicts between forage quality, forage quantity, and predator avoidance. Oecologia. 1988;75:580–586. doi: 10.1007/BF00776423. [DOI] [PubMed] [Google Scholar]
  • 64.Conradt L. Definitions, hypotheses, models and measures in the study of animal segregation. In: Ruckstuhl K, Neuhaus PJP, editors. Sexual segregation in vertebrates: ecology of the two sexes. Cambridge: Cambridge University Press; 2006. pp. 11–32. [Google Scholar]
  • 65.Lima SL, Dill LM. Behavioral decisions made under the risk of predation: a review and prospectus. Can J Zool. 1990;68:619–640. doi: 10.1139/z90-092. [DOI] [Google Scholar]
  • 66.Altendorf KB, Laundré JW, López González CA, Brown JS. Assessing effects of predation risk on foraging behavior of mule deer. J Mammal. 2001;82:430–439. doi: 10.1644/1545-1542(2001)082&#x0003c;0430:AEOPRO&#x0003e;2.0.CO;2. [DOI] [Google Scholar]
  • 67.Hernández L, Laundré JW. Foraging in the “landscape of fear” and its implications for habitat use and diet quality of elk Cervus elaphus and bison Bison bison. Wildl Biol. 2005;11:215–220. doi: 10.2981/0909-6396(2005)11[215:FITLOF]2.0.CO;2. [DOI] [Google Scholar]
  • 68.Wolff JO. Breeding strategies, mate choice, and reproductive success in American bison. Oikos. 1998;83:529–544. doi: 10.2307/3546680. [DOI] [Google Scholar]
  • 69.Bowyer RT, Bleich VC, Manteca X, Whiting JC, Stewart KM. Sociality, mate choice, and timing of mating in American bison (Bison bison): effects of large males. Ethology. 2007;113:1048–1060. doi: 10.1111/j.1439-0310.2007.01411.x. [DOI] [Google Scholar]
  • 70.Conradt L, Gordon IJ, Clutton-Brock TH, Thomson D, Guinness FE. Could the indirect competition hypothesis explain inter-sexual site segregation in red deer (Cervus elaphus L.)? J Zool. 2001;254:185–193. doi: 10.1017/S0952836901000693. [DOI] [Google Scholar]
  • 71.Conradt L, Clutton-Brock TH, Thomson D. Habitat segregation in ungulates: are males forced into suboptimal foraging habitats through indirect competition by females? Oecologia. 1999;119:367–377. doi: 10.1007/s004420050797. [DOI] [PubMed] [Google Scholar]
  • 72.Barboza PS, Bowyer RT. Sexual segregation in dimorphic deer: a new gastrocentric hypothesis. J Mammal. 2000;81:473–489. doi: 10.1644/1545-1542(2000)081&#x0003c;0473:SSIDDA&#x0003e;2.0.CO;2. [DOI] [Google Scholar]
  • 73.Schroeder CA, Bowyer RT, Bleich VC, Stephenson TR. Sexual segregation in Sierra Nevada bighorn sheep, Ovis canadensis sierrae: ramifications for conservation. Arct Antarct Alp Res. 2010;42:476–489. doi: 10.1657/1938-4246-42.4.476. [DOI] [Google Scholar]
  • 74.Miquelle DG. Why don’t bull moose eat during the rut? Behav Ecol Sociobiol. 1990;27:145–151. doi: 10.1007/BF00168458. [DOI] [Google Scholar]
  • 75.Darimont CT, Paquet PC, Reimchen TE. Stable isotopic niche predicts fitness of prey in a wolf–deer system. Biol J Linn Soc. 2007;90:125–137. doi: 10.1111/j.1095-8312.2007.00716.x. [DOI] [Google Scholar]
  • 76.Schwertl M, Auerswald K, Schäufele R, Schnyder H. Carbon and nitrogen stable isotope composition of cattle hair: ecological fingerprints of production systems? Agric Eco Environ. 2005;109:153–165. doi: 10.1016/j.agee.2005.01.015. [DOI] [Google Scholar]
  • 77.Feranec RS. Stable carbon isotope values reveal evidence of resource partitioning among ungulates from modern C3-dominated ecosystems in North America. Palaeogeogr Palaeoclimatol Palaeoecol. 2007;252:575–585. doi: 10.1016/j.palaeo.2007.05.012. [DOI] [Google Scholar]
  • 78.Singer FJ, Norland JE. Niche relationships within a guild of ungulate species in Yellowstone National Park, Wyoming, following release from artificial controls. Can J Zool. 1994;72:1383–1394. doi: 10.1139/z94-183. [DOI] [Google Scholar]
  • 79.Fox-Dobbs K, Bump JK, Peterson RO, Fox DL, Koch PL. Carnivore-specific stable isotope variables and variation in the foraging ecology of modern and ancient wolf populations: case studies from Isle Royale, Minnesota, and La Brea. Can J Zool. 2007;85:458–471. doi: 10.1139/Z07-018. [DOI] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The datasets analyzed in the current study are available from the corresponding author on reasonable request.

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