Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Nov;30(2):385–390. doi: 10.1128/iai.30.2.385-390.1980

Generation of chemiluminescence by human neutrophils exposed to soluble stimuli of oxidative metabolism.

M A Westrick, P S Shirley, L R DeChatelet
PMCID: PMC551323  PMID: 7439985

Abstract

The detection of chemiluminescence of human polymorphonuclear leukocytes activated by the nonparticulate stimulus phorbol myristate acetate required the presence of suitable substrate, such as protein or luminol, in the reaction medium. This substrate requirement was met by the addition of human serum or various proteins, such as bovine serum albumin, lysozyme, egg albumin, or immunoglobulin, to the reaction vial. Luminol, a chemiluminescent compound, could substitute for protein and markedly enhanced chemiluminescence by phorbol myristate acetate-induced and concanavalin A-induced polymorphonuclear leukocytes. From this work, it appears likely that soluble stimuli activate the polymorphonuclear leukocytes, but this activation, as measured by chemiluminescence, is not detectable in the absence of a secondary interaction with suitable components in the medium.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. C. Evaluation of serum opsonic capacity by quantitating the initial chemiluminescent response from phagocytizing polymorphonuclear leukocytes. Infect Immun. 1977 Mar;15(3):828–833. doi: 10.1128/iai.15.3.828-833.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen R. C., Loose L. D. Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochem Biophys Res Commun. 1976 Mar 8;69(1):245–252. doi: 10.1016/s0006-291x(76)80299-9. [DOI] [PubMed] [Google Scholar]
  3. Allen R. C., Stjernholm R. L., Steele R. H. Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem Biophys Res Commun. 1972 May 26;47(4):679–684. doi: 10.1016/0006-291x(72)90545-1. [DOI] [PubMed] [Google Scholar]
  4. Allen R. C., Yevich S. J., Orth R. W., Steele R. H. The superoxide anion and singlet molecular oxygen: their role in the microbicidal activity of the polymorphonuclear leukocyte. Biochem Biophys Res Commun. 1974 Oct 8;60(3):909–917. doi: 10.1016/0006-291x(74)90401-x. [DOI] [PubMed] [Google Scholar]
  5. Babior B. M. Oxygen-dependent microbial killing by phagocytes (first of two parts). N Engl J Med. 1978 Mar 23;298(12):659–668. doi: 10.1056/NEJM197803232981205. [DOI] [PubMed] [Google Scholar]
  6. Cheson B. D., Christensen R. L., Sperling R., Kohler B. E., Babior B. M. The origin of the chemiluminescence of phagocytosing granulocytes. J Clin Invest. 1976 Oct;58(4):789–796. doi: 10.1172/JCI108530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeChatelet L. R., Cooper M. R. A modified procedure for the determination of leukocyte alkaline phosphatase. Biochem Med. 1970 Aug;4(1):61–68. doi: 10.1016/0006-2944(70)90103-1. [DOI] [PubMed] [Google Scholar]
  8. DeChatelet L. R., Shirley P. S., Johnston R. B., Jr Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood. 1976 Apr;47(4):545–554. [PubMed] [Google Scholar]
  9. McPhail L. C., DeChatelet L. R., Johnston R. B., Jr Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events. J Clin Invest. 1979 Apr;63(4):648–655. doi: 10.1172/JCI109347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nelson R. D., Herron M. J., Schmidtke J. R., Simmons R. L. Chemiluminescence response of human leukocytes: influence of medium components on light production. Infect Immun. 1977 Sep;17(3):513–520. doi: 10.1128/iai.17.3.513-520.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stevens P., Winston D. J., Van Dyke K. In vitro evaluation of opsonic and cellular granulocyte function by luminol-dependent chemiluminescence: utility in patients with severe neutropenia and cellular deficiency states. Infect Immun. 1978 Oct;22(1):41–51. doi: 10.1128/iai.22.1.41-51.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Webb L. S., Keele B. B., Jr, Johnston R. B., Jr Inhibition of phagocytosis-associated chemiluminescence by superoxide dismutase. Infect Immun. 1974 Jun;9(6):1051–1056. doi: 10.1128/iai.9.6.1051-1056.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES