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. 2017 Apr 7;8(23):36950–36963. doi: 10.18632/oncotarget.16939

Figure 7. The effect of NAC on lifespan shortening and lipofuscin accumulation in kin-10 RNAi worms.

Figure 7

Age-synchronized L4 larvae were fed on empty vector control (L4440) plates or kin-10 RNAi plates containing NAC (0, 3, or 6 µM) under standard conditions. A. Viability was scored as movement away from pick touch at the indicated days. Representative data from three independent RNAi experiments are shown (n = 50 per condition). B. Representative autofluorescence images of worms at day 1 of adulthood (upper panel). The fluorescence intensity (bottom panel) was quantified using the ImageJ software by determining the average pixel intensity (n > 40 per condition). Data are shown as the means ± SEM. **P < 0.01; ***P < 0.001. All experiments in this figure were carried out at 21 °C.