Abstract
C57BL/10 mice have previously been shown to be 100 times more resistant to intravenously injected Listeria monocytogenes than are BALB/c mice due to the action of a single gene, Lr. Differences in the histopathology of listeriosis in the two strains were sought. Of the tissues examined, only liver, spleen, blood, and thymus showed changes. In the liver, Listeria localized in Kupffer cells within 3 h of infection. By 24 h these cells became surrounded by neutrophilic polymorphonuclear leukocytes. After high doses of Listeria, the susceptible BALB/c mice showed many foci surrounded by few polymorphs, whereas in the resistant C57BL/10 mice there were relatively few foci surrounded by many polymorphs. By 4 days in sublethally infected mice the polymorphs in the liver of both strains were being replaced by monocytes and macrophages. Liver morphology returned to normal by 8 days postinfection. In the blood of both strains there was a rise in total lymphocyte numbers at 24 h, followed by a fall in T-lymphocytes and recovery at 5 days. C57BL/10 mice showed an early monocytic response in the blood, whereas BALB/c mice showed a polymorph leukocytosis. In the spleens of both C57BL/10 and BALB/c mice there was an early neutrophil response and red pulp hyperemia. This was followed by a dramatic lymphocyte depletion in the T-dependent periarteriolar regions in both strains beginning 2 days after infection. Absolute numbers of Thy-1+ cells in spleen cell suspensions also fell to 10% of normal, recovering 6 to 8 days postinfection. Surface immunoglobulin-positive B-lymphocytes and Thy-1−, immunoglobulin-negative “null” cells rose in both strains at days 4 to 5, returning to normal levels on days 10 to 12. Whether the null cells represent lymphocytes or other cell types remains unresolved. Thymus atrophy was seen in the BALB/c mice but not in C57BL/10 mice.
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Selected References
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- Askonas B. A., Corsini A. C., Clayton C. E., Ogilvie B. M. Functional depletion of T- and B-memory cells and other lymphoid cell subpopulations-during trypanosomiasis. Immunology. 1979 Feb;36(2):313–321. [PMC free article] [PubMed] [Google Scholar]
- Bennett M., Baker E. E. Marrow-dependent cell function in early stages of infection with Listeria monocytogenes. Cell Immunol. 1977 Sep;33(1):203–210. doi: 10.1016/0008-8749(77)90147-2. [DOI] [PubMed] [Google Scholar]
- Cheers C., McKenzie I. F., Pavlov H., Waid C., York J. Resistance and susceptibility of mice to bacterial infection: course of listeriosis in resistant or susceptible mice. Infect Immun. 1978 Mar;19(3):763–770. doi: 10.1128/iai.19.3.763-770.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheers C., McKenzie I. F. Resistance and susceptibility of mice to bacterial infection: genetics of listeriosis. Infect Immun. 1978 Mar;19(3):755–762. doi: 10.1128/iai.19.3.755-762.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen J. J., Rodriguez G. E., Kind P. D., Campbell P. A. Listeria cell wall fraction: a B cell mitogen. J Immunol. 1975 Mar;114(3):1132–1134. [PubMed] [Google Scholar]
- Dwyer J. M., Bullock W. E., Fields J. P. Disturbance of the blood T:B lymphocyte ratio in lepromatous leprosy. Clinical and immunologic correlations. N Engl J Med. 1973 May 17;288(20):1036–1039. doi: 10.1056/NEJM197305172882002. [DOI] [PubMed] [Google Scholar]
- Fowles R. E., Fajardo I. M., Leibowitch J. L., David J. R. The enhancement of macrophage bacteriostasis by products of activated lymphocytes. J Exp Med. 1973 Oct 1;138(4):952–964. doi: 10.1084/jem.138.4.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lannigan R., Skamene E., Kongshavn P. A., Duguid W. P. Morphology of liver lesions in experimental listeriosis is splenectomized mice. J Reticuloendothel Soc. 1979 May;25(5):457–461. [PubMed] [Google Scholar]
- MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MIKI K., MACKANESS G. B. THE PASSIVE TRANSFER OF ACQUIRED RESISTANCE TO LISTERIA MONOCYTOGENES. J Exp Med. 1964 Jul 1;120:93–103. doi: 10.1084/jem.120.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer E. M., Grundmann E. BCG-induced changes in size of thymic cortex and thymus-dependent areas in spleen and lymph nodes of mice. Clin Exp Immunol. 1980 Jan;39(1):60–65. [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Sprent J. Cell-to-cell interaction in the immune response. VI. Contribution of thymus-derived cells and antibody-forming cell precursors to immunological memory. J Exp Med. 1971 Jul 1;134(1):66–82. doi: 10.1084/jem.134.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. K., Burns J. Histopathology of Listeria monocytogenes after oral feeding to mice. Appl Microbiol. 1970 May;19(5):772–775. doi: 10.1128/am.19.5.772-775.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nathan C. F., Remold H. G., David J. R. Characterization of a lymphocyte factor which alters macrophage functions. J Exp Med. 1973 Feb 1;137(2):275–290. doi: 10.1084/jem.137.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J. Cellular kinetics associated with the development of acquired cellular resistance. J Exp Med. 1969 Aug 1;130(2):299–314. doi: 10.1084/jem.130.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J., Deissler J. F. Nature of "memory" in T-cell mediated antibacterial immunity: cellular parameters that distinguish between the active immune response and a state of "memory". Infect Immun. 1975 Oct;12(4):761–767. doi: 10.1128/iai.12.4.761-767.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J. The action of cortisone acetate on cell-mediated immunity to infection: histogenesis of the lymphoid cell response and selective elimination of committed lymphocytes. Cell Immunol. 1972 Mar;3(3):501–515. doi: 10.1016/0008-8749(72)90255-9. [DOI] [PubMed] [Google Scholar]
- North R. J. The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J Exp Med. 1970 Sep 1;132(3):521–534. doi: 10.1084/jem.132.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skamene E., Kongshavn P. A., Sachs D. H. Resistance to Listeria monocytogenes in mice: genetic control by genes that are not linked to the H-2 complex. J Infect Dis. 1979 Feb;139(2):228–231. doi: 10.1093/infdis/139.2.228. [DOI] [PubMed] [Google Scholar]
- Tandon P., Chaturvedi U. C., Mathur A. Differential depletion of T lymphocytes in the spleen of dengue virus-infected mice. Immunology. 1979 May;37(1):1–6. [PMC free article] [PubMed] [Google Scholar]
- Tatsukawa K., Mitsuyama M., Takeya K., Nomoto K. Differing contribution of polymorphonuclear cells and macrophages to protection of mice against Listeria monocytogenes and Pseudomonas aeruginosa. J Gen Microbiol. 1979 Nov;115(1):161–166. doi: 10.1099/00221287-115-1-161. [DOI] [PubMed] [Google Scholar]
- Turk J. L., Waters M. F. Immunological basis for depression of cellular immunity and the delayed allergic response in patients with lepromatous leprosy. Lancet. 1968 Aug 24;2(7565):436–438. doi: 10.1016/s0140-6736(68)90472-8. [DOI] [PubMed] [Google Scholar]