Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Jun;36(3):857–863. doi: 10.1128/iai.36.3.857-863.1982

Conditions required for induction of interferon by rotaviruses and for their sensitivity to its action.

J L McKimm-Breschkin, I H Holmes
PMCID: PMC551408  PMID: 6178689

Abstract

Our investigations of interferon induction by rotaviruses showed that only when cells were pretreated with interferon, i.e., primed, could infectious rotaviruses induce significant quantities of interferon. As little as 0.5 U of interferon provided sufficient priming for this induction. UV-irradiated rotaviruses induced significant levels of interferon, and priming only marginally enhanced the yields. Neither heat-inactivated virus nor serum-neutralized virus was able to induce interferon, even when cells were primed. When cells were treated with purified virus double-stranded RNA in the presence of DEAE-dextran to facilitate uptake, interferon was induced, although priming did not enhance yields. These results strongly implicate the viral double-stranded RNA as the effector for interferon induction. The insensitivity of rotaviruses to interferon in vitro was also studied. Results suggested that this lack of sensitivity was not due to any inherent resistance of the virus to the antiviral proteins, but rather to lack of activation of cellular enzymes exhibiting antiviral activity.

Full text

PDF
857

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins G. J., Johnston M. D., Westmacott L. M., Burke D. C. Department of Biological Sciences, University of Warwick, Coventry, CV47AL, England. J Gen Virol. 1974 Dec;25(3):381–390. doi: 10.1099/0022-1317-25-3-381. [DOI] [PubMed] [Google Scholar]
  2. Atkins G. J., Lancashire C. L. The induction of interferon by temperature-sensitive mutants of Sindbis virus: its relationship to double-stranded RNA synthesis and cytopathic effect. J Gen Virol. 1976 Feb;30(2):157–165. doi: 10.1099/0022-1317-30-2-157. [DOI] [PubMed] [Google Scholar]
  3. Baglioni C., Minks M. A., Maroney P. A. Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A. Nature. 1978 Jun 22;273(5664):684–687. doi: 10.1038/273684a0. [DOI] [PubMed] [Google Scholar]
  4. CANTELL K., PAUCKER K. QUANTITATIVE STUDIES ON VIRAL INTERFERENCE IN SUSPENDED L CELLS. IV. PRODUCTION AND ASSAY OF INTERFERON. Virology. 1963 Sep;21:11–21. doi: 10.1016/0042-6822(63)90298-8. [DOI] [PubMed] [Google Scholar]
  5. Cohen J. Ribonucleic acid polymerase activity associated with purified calf rotavirus. J Gen Virol. 1977 Sep;36(3):395–402. doi: 10.1099/0022-1317-36-3-395. [DOI] [PubMed] [Google Scholar]
  6. De Clercq E., Merigan T. C. Current concepts of interferon and interferon induction. Annu Rev Med. 1970;21:17–46. doi: 10.1146/annurev.me.21.020170.000313. [DOI] [PubMed] [Google Scholar]
  7. Friedman R. M. Effect of interferon treatment on interferon production. J Immunol. 1966 May;96(5):872–877. [PubMed] [Google Scholar]
  8. Henderson D. R., Joklik W. K. The mechanism of interferon induction by UV-irradiated reovirus. Virology. 1978 Dec;91(2):389–406. doi: 10.1016/0042-6822(78)90386-0. [DOI] [PubMed] [Google Scholar]
  9. Holmes I. H. Viral gastroenteritis. Prog Med Virol. 1979;25:1–36. [PubMed] [Google Scholar]
  10. Hovanessian A. G., Wood J., Meurs E., Montagnier L. Increased nuclease activity in cells treated with pppA2'p5'A2'p5' A. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3261–3265. doi: 10.1073/pnas.76.7.3261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ISAACS A., LINDENMANN J. Virus interference. I. The interferon. Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):258–267. doi: 10.1098/rspb.1957.0048. [DOI] [PubMed] [Google Scholar]
  12. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., van Kirk D. H., Chanock R. M., Kapikian A. Z. Differentiation of human and calf reoviruslike agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. Virology. 1976 Oct 1;74(1):86–92. doi: 10.1016/0042-6822(76)90131-8. [DOI] [PubMed] [Google Scholar]
  13. Kerr I. M., Brown R. E., Hovanessian A. G. Nature of inhibitor of cell-free protein synthesis formed in response to interferon and double-stranded RNA. Nature. 1977 Aug 11;268(5620):540–542. doi: 10.1038/268540a0. [DOI] [PubMed] [Google Scholar]
  14. Kerr I. M., Brown R. E. pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells. Proc Natl Acad Sci U S A. 1978 Jan;75(1):256–260. doi: 10.1073/pnas.75.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kleinschmidt W. J. Biochemistry of interferon and its inducers. Annu Rev Biochem. 1972;41(10):517–542. doi: 10.1146/annurev.bi.41.070172.002505. [DOI] [PubMed] [Google Scholar]
  16. La Bonnardiere C., de Vaureix C., L'Haridon R., Scherrer R. Weak susceptibility of rotavirus to bovine interferon in calf kidney cells. Arch Virol. 1980;64(2):167–170. doi: 10.1007/BF01318020. [DOI] [PubMed] [Google Scholar]
  17. Lockart R. Z., Jr, Bayliss N. L., Toy S. T., Yin F. H. Viral events necessary for the induction of interferon in chick embryo cells. J Virol. 1968 Oct;2(10):962–965. doi: 10.1128/jvi.2.10.962-965.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lomniczi B., Burke D. C. Interferon production by temperature-sensitive mutants of Semliki Forest virus. J Gen Virol. 1970 Jul;8(1):55–68. doi: 10.1099/0022-1317-8-1-55. [DOI] [PubMed] [Google Scholar]
  19. McNulty M. S. Rotaviruses. J Gen Virol. 1978 Jul;40(1):1–18. doi: 10.1099/0022-1317-40-1-1. [DOI] [PubMed] [Google Scholar]
  20. Paucker K., Boxaca M. Cellular resistance to induction of interferon. Bacteriol Rev. 1967 Jun;31(2):145–156. doi: 10.1128/br.31.2.145-156.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shepherd R. W., Truslow S., Walker-Smith J. A., Bird R., Cutting W., Darnell R., Barker C. M. Infantile gastroenteritis: a clinical study of reovirus-like agent infection. Lancet. 1975 Nov 29;2(7944):1082–1084. doi: 10.1016/s0140-6736(75)90446-8. [DOI] [PubMed] [Google Scholar]
  22. Sonza S., Holmes I. H. Coproantibody response to rotavirus infection. Med J Aust. 1980 Nov 1;2(9):496–499. doi: 10.5694/j.1326-5377.1980.tb100710.x. [DOI] [PubMed] [Google Scholar]
  23. Thacore H., Youngner J. S. Cells persistently infected with Newcastle disease virus. II. Ribonucleic acid and protein synthesis in cells infected with mutants isolated from persistently infected L cells. J Virol. 1970 Jul;6(1):42–48. doi: 10.1128/jvi.6.1.42-48.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Welch A. B., Twiehaus M. J. Cell culture studies of a neonatal calf diarrhea virus. Can J Comp Med. 1973 Jul;37(3):287–294. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES