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. 1982 Jun;36(3):1209–1216. doi: 10.1128/iai.36.3.1209-1216.1982

In vivo induction of anti-herpes simplex virus immune response by type 1 antigens and lipid A incorporated into liposomes.

P T Naylor, H S Larsen, L Huang, B T Rouse
PMCID: PMC551458  PMID: 6284643

Abstract

To establish the requirements for a potential subunit vaccine against herpes simplex virus (HSV), we analyzed the effects on immunogenicity of incorporating detergent-extracted glycoprotein-enriched HSV type 1 (HSV-1) antigens into liposomes alone or with the adjuvant lipid A. Incorporating HSV-1 antigens into liposomes enhanced their immunogenicity for antibody production as detected by radioimmunoassay. Antibody levels to free and liposome-bound antigens were enhanced by administering lipid A as an adjuvant. The maximum immunogenic effect was obtained by incorporating lipid A into liposomes containing the HSV-1 proteins. Such liposomes induced secondary antibody responses higher than those engendered by virus infection. Whereas infectious virus induced cell-mediated immunity detectable by the delayed-type hypersensitivity reactions and cytotoxic T lymphocyte production, none of the liposome preparations induced cell-mediated immunity.

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Selected References

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