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. 2017 Apr 8;8(23):36984–36995. doi: 10.18632/oncotarget.16974

Figure 3. Anti-adipogenic effect of zerumbone in 3T3-L1 cells.

Figure 3

A. Effect of zerumbone on cell viability after 48 h exposure. B. Oil Red O staining of 3T3-L1 cells that were differentiated for 10 days. Cells were treated with 5 or 10 μM zerumbone for 48 h at day 0. Non-differentiated preadipocytes (CS) were incubated in DMEM with 10% calf serum. Representative photomicrographs (×200) are shown for each treatment group. C. Intracellular lipid accumulation was quantified by measuring optical absorbance at 500 nm. D. Real-time PCR analysis of adipogenic transcription factors and their targets in 3T3-L1 cells. The level of each mRNA is expressed relative to the average level of CS. *P < 0.05, **P < 0.01 versus differentiated adipocytes. E. Western blotting was performed for various adipocyte differentiation markers.