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. 2017 Jun 14;6:e24998. doi: 10.7554/eLife.24998

Table 1.

Summary of kinetic analysis of ARPP-16 phosphorylation by MAST3 and PKA.

DOI: http://dx.doi.org/10.7554/eLife.24998.004


MAST3 Vm Km Catalytic efficiency
32P-incorporation/minute nM Vm/Km
Figure 1b ARPP-16 1388 ± 27 90 ± 5 15.4

P-S88-ARPP-16 866 ± 59 158 ± 26 5.5
Figure 1—figure supplement 1 ARPP-16 1391 ± 34 92 ± 6 15.1

P-S88D-ARPP-16 465 ± 32 138 ± 24 3.4

PKA Vm Km Catalytic efficiency

32P-incorporation/minute nM Vm/Km
Figure 1d ARPP-16 6999 ± 127 1685 ± 51 4.1

P-S46-ARPP-16 1027 ± 39 870 ± 47 1.2