FIG 1 .

MGE1 overexpression improves growth of the wild-type S. cerevisiae strain on fluconazole. (A) Etest analysis of the overexpression strain (MGE1) and control strain (EV). (B) Serial dilutions of both strains were spotted on SDglu medium containing fluconazole (flu; 10 or 20 μg/ml) and/or doxycycline (dox; 50 or 100 μg/ml). Pictures were taken after 48 and 72 h of incubation at 30°C. (C) Tolerance assay. Data represent dose-response curves determined for both strains, with dotted lines indicating 50% (upper line) and 90% (middle line) growth inhibition and the initial inoculum (lower line). No significant difference was observed in trailing growth between the overexpression strain and control strain (P = 0.731 for 128 μg/ml flu and P = 0.381 for 64 μg/ml flu, tested by two-way ANOVA with Bonferroni correction).