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. 1990 May;9(5):1465–1469. doi: 10.1002/j.1460-2075.1990.tb08263.x

Bacteriorhodopsin drives the glutamate transporter of synaptic vesicles after co-reconstitution.

P R Maycox 1, T Deckwerth 1, R Jahn 1
PMCID: PMC551836  PMID: 1970294

Abstract

Active accumulation of neurotransmitters by synaptic vesicles is an essential component of the synaptic transmission cycle. Isolated vesicles show energy-dependent uptake of several transmitters by processes which are apparently mediated by a proton electrochemical potential across the vesicle membrane. Although this energy gradient is probably generated by a proton ATPase, the functional separation of ATP cleavage and transmitter uptake activity has only been shown clearly for monoamine transport. We report here that the light-driven proton pump, bacteriorhodopsin, can replace the endogenous proton ATPase in proteoliposomes reconstituted from vesicular detergent extracts. The system shows light-dependent uptake of glutamate with properties very similar to those observed in intact vesicles, e.g. chloride dependence or stimulation by NH4+. Our experiments show that the proton pump and the glutamate transporter are separate entities and provide a powerful tool for further characterization of the glutamate carrier.

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Selected References

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