Abstract
A sesquidiploid hybrid having two genomes of Lycopersicon esculentum and one of Solanum lycopersicoides served as a pistillate bridging parent in crosses with Solanum rickii to produce L. esculentum x S. rickii hybrid progeny. Of the four progeny obtained, one (GH2754) was diploid and three were aneuploid with extra S. lycopersicoides chromosomes. The hybrids had morphological features of both parents, but attributes of the wild parent dominated. The hybrid nature of the four progeny was confirmed by isozyme, restriction fragment length polymorphism, and cytological analyses. A mean of 9.15 bivalents was observed in pollen mother cells of GH2754. A high level of pollen abortion was seen in all hybrids. Crosses of the hybrids with staminate S. rickii yielded one backcross individual, revealing a very low, but certain level of female fertility. Colchicine treatment of GH2754 generated one promising amphidiploid hybrid, which exhibited strong preferential chromosome pairing (94% of the examined cells had 24 bivalents) and appreciable pollen fertility (43% stainable). Chromosome pairing, isozyme, and restriction fragment length polymorphism data support a very close relationship between the two Solanum spp. and a much greater distance between them and L. esculentum, but the data do not discriminate between them in respect to their distances from the latter. The cytological and molecular observations, previous reports of successful transfer of traits from S. lycopersicoides to L. esculentum, and our hybridization of L. esculentum x S. rickii suggest good prospects for gene transfer from S. rickii to L. esculentum.
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- Bonierbale M. W., Plaisted R. L., Tanksley S. D. RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato. Genetics. 1988 Dec;120(4):1095–1103. doi: 10.1093/genetics/120.4.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Menzel M Y. Preferential Chromosome Pairing in Allotetraploid Lycopersicon Esculentum-solanum Lycopersicoides. Genetics. 1964 Nov;50(5):855–862. doi: 10.1093/genetics/50.5.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rick C. M., De Verna J. W., Chetelat R. T., Stevens M. A. Meiosis in sesquidiploid hybrids of Lycopersicon esculentum and Solanum lycopersicoides. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3580–3583. doi: 10.1073/pnas.83.11.3580. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rick C. M. Hybrids Between Lycopersicon Esculentum Mill. And Solanum Lycopersicoides Dun. Proc Natl Acad Sci U S A. 1951 Nov;37(11):741–744. doi: 10.1073/pnas.37.11.741. [DOI] [PMC free article] [PubMed] [Google Scholar]