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. 2017 Jun 12;6(6):e345. doi: 10.1038/oncsis.2017.44

Figure 1.

Figure 1

Ectopic expression of MLK3 drives FRA-1 expression in ER+ BC cells. Cellular lysates and/or mRNAs were collected from (a and b) MCF7iMLK3 cells treated with vehicle or 50 nm AP21967 to induce MLK3 expression for 24 h, (c) MCF7 cells were transiently transfected with a wild-type MLK3 (pRK-MLK3) or a kinase dead MLK3 variant (pRK-MLK3-K144M) for 24 h, and (d) ZR-75-1 cells transiently transfected with pRK-MLK3 expression vector for 24 h. Cellular lysates were subjected to immunoblotting with indicated antibodies. Western blot quantification of the indicated protein normalized to actin is expressed as mean±s.d. from at least three independent experiments. The mRNAs were subjected to qRT–PCR with primers for the indicated genes. Relative mRNA expression is displayed as mean±s.d. from at least three independent experiments performed in triplicate; NS, not statistically significant; **P<0.01.